Program: 15. Cancer Associated Fibroblast.

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QQ-plot of gene loadings, averaged over both independent splits of the data
This plot highlights the relative contribution of each gene to the GEP

Interactive QQ Plot

Top genes driving this program. Note: Decartes website is buggy, try refreshing. Also, Decartes fetal adrenal data have been collected at specific time points (89-122 days), all possible cell types of interest may not be represented, do not overinterpret. The Mean Count column shows the mean read count in cells scoring highly (H > 50) on this gene expression program (currently only calculated for the cells in the first 50/50 split of the data). Note: Decartes website is buggy, try refreshing. Also, Decartes fetal adrenal data have been collected at specific time points (89-122 days), all possible cell types of interest may not be represented, do not overinterpret.
The Mean Count column shows the mean read count in cells scoring highly (H > 50) on this gene expression program.

	Gene	Loading	Gene.Name	GTEx	DepMap	Descartes	Mean.Counts	Mean.Tpm
1	CYP1B1	0.0105560	cytochrome P450 family 1 subfamily B member 1	GTEx	DepMap	Descartes	31.88	1734.29
2	EMP1	0.0094562	epithelial membrane protein 1	GTEx	DepMap	Descartes	39.52	1947.89
3	SPRY2	0.0094386	sprouty RTK signaling antagonist 2	GTEx	DepMap	Descartes	8.42	925.09
4	LIF	0.0086612	LIF interleukin 6 family cytokine	GTEx	DepMap	Descartes	15.10	1147.66
5	HMOX1	0.0085386	heme oxygenase 1	GTEx	DepMap	Descartes	17.55	3322.44
6	DIO2	0.0083648	iodothyronine deiodinase 2	GTEx	DepMap	Descartes	5.40	260.46
7	CCL2	0.0080405	C-C motif chemokine ligand 2	GTEx	DepMap	Descartes	69.43	21560.64
8	LUM	0.0079736	lumican	GTEx	DepMap	Descartes	48.81	4979.99
9	F3	0.0070846	coagulation factor III, tissue factor	GTEx	DepMap	Descartes	6.59	888.37
10	TXNRD1	0.0070366	thioredoxin reductase 1	GTEx	DepMap	Descartes	6.87	475.12
11	BHLHE41	0.0068776	basic helix-loop-helix family member e41	GTEx	DepMap	Descartes	2.41	186.25
12	FRMD6	0.0068186	FERM domain containing 6	GTEx	DepMap	Descartes	4.72	234.68
13	SLC4A7	0.0067690	solute carrier family 4 member 7	GTEx	DepMap	Descartes	5.51	203.18
14	CDC42EP2	0.0067468	CDC42 effector protein 2	GTEx	DepMap	Descartes	1.70	232.69
15	TENM2	0.0067411	teneurin transmembrane protein 2	GTEx	DepMap	Descartes	4.03	NA
16	THBS1	0.0067087	thrombospondin 1	GTEx	DepMap	Descartes	38.07	1452.17
17	S1PR3	0.0066703	sphingosine-1-phosphate receptor 3	GTEx	DepMap	Descartes	6.04	202.18
18	DCN	0.0066557	decorin	GTEx	DepMap	Descartes	65.98	2707.49
19	EGFR	0.0065333	epidermal growth factor receptor	GTEx	DepMap	Descartes	6.70	211.65
20	CLDN1	0.0065323	claudin 1	GTEx	DepMap	Descartes	5.07	462.84
21	LAMA2	0.0065236	laminin subunit alpha 2	GTEx	DepMap	Descartes	6.35	NA
22	FN1	0.0064211	fibronectin 1	GTEx	DepMap	Descartes	26.36	784.78
23	TNFRSF12A	0.0063445	TNF receptor superfamily member 12A	GTEx	DepMap	Descartes	4.94	855.81
24	TIPARP	0.0063319	TCDD inducible poly(ADP-ribose) polymerase	GTEx	DepMap	Descartes	8.83	589.92
25	FRMD4A	0.0062592	FERM domain containing 4A	GTEx	DepMap	Descartes	3.68	132.51
26	CFH	0.0061403	complement factor H	GTEx	DepMap	Descartes	10.59	453.59
27	SERPINE1	0.0061179	serpin family E member 1	GTEx	DepMap	Descartes	32.71	2593.16
28	PHLDA1	0.0060217	pleckstrin homology like domain family A member 1	GTEx	DepMap	Descartes	15.09	474.22
29	PRNP	0.0059731	prion protein	GTEx	DepMap	Descartes	8.87	1037.29
30	RTN4	0.0058423	reticulon 4	GTEx	DepMap	Descartes	14.01	864.57
31	MEST	0.0057630	mesoderm specific transcript	GTEx	DepMap	Descartes	3.47	319.00
32	GCLM	0.0057439	glutamate-cysteine ligase modifier subunit	GTEx	DepMap	Descartes	4.15	254.81
33	LIMA1	0.0055970	LIM domain and actin binding 1	GTEx	DepMap	Descartes	4.05	279.62
34	UGDH	0.0055700	UDP-glucose 6-dehydrogenase	GTEx	DepMap	Descartes	6.68	645.30
35	GSN	0.0055647	gelsolin	GTEx	DepMap	Descartes	22.16	1025.64
36	CHSY1	0.0055561	chondroitin sulfate synthase 1	GTEx	DepMap	Descartes	4.79	292.61
37	CTSL	0.0055010	cathepsin L	GTEx	DepMap	Descartes	10.97	NA
38	BMP8B	0.0054665	bone morphogenetic protein 8b	GTEx	DepMap	Descartes	1.54	87.59
39	CLMP	0.0054332	CXADR like membrane protein	GTEx	DepMap	Descartes	2.20	130.18
40	PAMR1	0.0053871	peptidase domain containing associated with muscle regeneration 1	GTEx	DepMap	Descartes	2.29	159.92
41	RND3	0.0053463	Rho family GTPase 3	GTEx	DepMap	Descartes	8.73	884.18
42	OLFML2B	0.0053294	olfactomedin like 2B	GTEx	DepMap	Descartes	6.70	566.55
43	FGFR1	0.0052568	fibroblast growth factor receptor 1	GTEx	DepMap	Descartes	11.87	555.91
44	OGN	0.0052020	osteoglycin	GTEx	DepMap	Descartes	7.42	678.65
45	TFPI	0.0051289	tissue factor pathway inhibitor	GTEx	DepMap	Descartes	5.16	196.80
46	TM4SF1	0.0050563	transmembrane 4 L six family member 1	GTEx	DepMap	Descartes	19.49	1721.22
47	GPC3	0.0050450	glypican 3	GTEx	DepMap	Descartes	4.20	536.32
48	KLHL21	0.0049656	kelch like family member 21	GTEx	DepMap	Descartes	5.97	260.05
49	ANXA1	0.0049648	annexin A1	GTEx	DepMap	Descartes	19.94	2433.59
50	ZBTB21	0.0049062	zinc finger and BTB domain containing 21	GTEx	DepMap	Descartes	3.51	NA

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UMAP plots showing activity of gene expression program identified in community:15. Cancer Associated Fibroblast

Interactive UMAP Plot

Boxlot showing activity of gene expression program identified in GEP 15. Cancer Associated Fibroblast:

Interactive Boxplot

CNV Data procured from inferCNV.
Outer tracks are putative CNV regions (gains = red, losses = blue) for each patient
Inner track is expression data representing:
The top cells expressing this GEP (purple)
Random cells (n =50) from the reference set used in inferCNV (orange)

Interactive BioCircos Plot

Gene set Enrichments for this program, caculated from top 50 genes

mSigDB Cell Types Gene Set:

	P-value	OR	Lower 95% CI	FDR	FWER	Genes Found	Gene Set Size
TRAVAGLINI_LUNG_ADVENTITIAL_FIBROBLAST_CELL	6.95e-19	27.66	14.57	4.66e-16	4.66e-16	19CYP1B1, EMP1, CCL2, LUM, F3, DCN, TNFRSF12A, TIPARP, CFH, RTN4, LIMA1, UGDH, GSN, CTSL, CLMP, PAMR1, FGFR1, OGN, ANXA1	296
CUI_DEVELOPING_HEART_LEFT_VENTRICULAR_CARDIOMYOCYTE	4.77e-07	39.59	11.58	3.18e-05	3.20e-04	5CCL2, PHLDA1, TFPI, TM4SF1, ANXA1	40
RUBENSTEIN_SKELETAL_MUSCLE_FAP_CELLS	3.15e-12	23.61	11.04	4.23e-10	2.12e-09	12EMP1, LUM, DCN, LAMA2, FN1, CFH, RTN4, LIMA1, GSN, RND3, TFPI, ANXA1	179
AIZARANI_LIVER_C21_STELLATE_CELLS_1	1.71e-10	19.26	8.76	1.91e-08	1.15e-07	11EMP1, CCL2, LUM, THBS1, DCN, TIPARP, CFH, SERPINE1, PHLDA1, GSN, RND3	194
GAO_STOMACH_24W_C4_PARIETAL_PROGENITOR	4.28e-04	86.37	8.33	8.21e-03	2.88e-01	2CFH, ANXA1	8
FAN_OVARY_CL1_GPRC5A_TNFRS12A_HIGH_SELECTABLE_FOLLICLE_STROMAL_CELL	4.49e-13	16.16	8.25	7.53e-11	3.01e-10	16EMP1, LUM, TXNRD1, DCN, TNFRSF12A, CFH, PHLDA1, PRNP, RTN4, GCLM, UGDH, GSN, CTSL, FGFR1, OGN, GPC3	380
MURARO_PANCREAS_MESENCHYMAL_STROMAL_CELL	1.39e-13	12.63	6.76	3.10e-11	9.30e-11	20CYP1B1, EMP1, LIF, LUM, TXNRD1, FRMD6, SLC4A7, THBS1, S1PR3, DCN, LAMA2, FN1, TNFRSF12A, SERPINE1, PHLDA1, CHSY1, CTSL, RND3, TFPI, ANXA1	680
HAY_BONE_MARROW_STROMAL	9.34e-14	12.18	6.57	3.10e-11	6.26e-11	21CYP1B1, EMP1, CCL2, LUM, FRMD6, TENM2, S1PR3, DCN, EGFR, FN1, TNFRSF12A, FRMD4A, CFH, LIMA1, GSN, CLMP, RND3, OLFML2B, FGFR1, TFPI, TM4SF1	765
GAO_LARGE_INTESTINE_24W_C1_DCLK1POS_PROGENITOR	2.37e-06	18.31	6.23	1.14e-04	1.59e-03	6CCL2, LUM, DCN, LAMA2, PAMR1, OGN	99
HU_FETAL_RETINA_FIBROBLAST	1.54e-09	11.81	5.71	1.48e-07	1.03e-06	13LUM, FRMD6, S1PR3, DCN, EGFR, FN1, FRMD4A, MEST, LIMA1, CLMP, FGFR1, OGN, TFPI	385
DURANTE_ADULT_OLFACTORY_NEUROEPITHELIUM_FIBROBLASTS_STROMAL_CELLS	1.72e-05	18.02	5.44	5.87e-04	1.15e-02	5CYP1B1, CCL2, LUM, DCN, GSN	82
AIZARANI_LIVER_C39_EPCAM_POS_BILE_DUCT_CELLS_4	5.22e-07	13.53	5.40	3.18e-05	3.50e-04	8LIF, CCL2, THBS1, EGFR, CLDN1, TNFRSF12A, RTN4, TM4SF1	184
DESCARTES_FETAL_EYE_STROMAL_CELLS	2.69e-05	16.33	4.94	7.86e-04	1.81e-02	5LUM, THBS1, DCN, FN1, OGN	90
DESCARTES_FETAL_HEART_STROMAL_CELLS	3.24e-04	25.71	4.86	6.82e-03	2.17e-01	3LUM, DCN, GPC3	34
CUI_DEVELOPING_HEART_COMPACT_VENTRICULAR_CARDIOMYOCYTE	1.24e-04	17.81	4.52	3.19e-03	8.31e-02	4LUM, DCN, FN1, OGN	65
DESCARTES_FETAL_THYMUS_STROMAL_CELLS	1.53e-05	13.01	4.45	5.70e-04	1.03e-02	6LUM, S1PR3, DCN, MEST, OGN, GPC3	137
TRAVAGLINI_LUNG_BASAL_CELL	7.97e-06	11.24	4.21	3.57e-04	5.35e-03	7F3, DCN, EGFR, CLDN1, CFH, PRNP, GPC3	188
TRAVAGLINI_LUNG_LIPOFIBROBLAST_CELL	2.19e-05	12.17	4.17	6.99e-04	1.47e-02	6CYP1B1, SPRY2, TIPARP, PHLDA1, UGDH, RND3	146
AIZARANI_LIVER_C20_LSECS_3	1.73e-06	9.59	4.06	9.65e-05	1.16e-03	9EMP1, SLC4A7, SERPINE1, LIMA1, CHSY1, CTSL, FGFR1, TFPI, TM4SF1	295
FAN_EMBRYONIC_CTX_BRAIN_ENDOTHELIAL_2	2.14e-06	9.33	3.95	1.11e-04	1.44e-03	9LUM, FRMD6, S1PR3, DCN, LAMA2, FN1, CFH, LIMA1, TFPI	303

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mSigDB Hallmark Gene Sets:

	P-value	OR	Lower 95% CI	FDR	FWER	Genes Found	Gene Set Size
HALLMARK_COAGULATION	1.05e-06	15.53	5.78	2.61e-05	5.23e-05	7F3, THBS1, FN1, CFH, SERPINE1, GSN, ANXA1	138
HALLMARK_EPITHELIAL_MESENCHYMAL_TRANSITION	9.73e-07	12.40	4.96	2.61e-05	4.87e-05	8LUM, THBS1, DCN, LAMA2, FN1, TNFRSF12A, SERPINE1, MEST	200
HALLMARK_APOPTOSIS	2.90e-06	13.21	4.94	4.84e-05	1.45e-04	7EMP1, HMOX1, LUM, DCN, TNFRSF12A, GSN, ANXA1	161
HALLMARK_HYPOXIA	1.19e-05	10.54	3.95	1.48e-04	5.94e-04	7HMOX1, F3, DCN, EGFR, TIPARP, SERPINE1, GPC3	200
HALLMARK_REACTIVE_OXYGEN_SPECIES_PATHWAY	9.53e-04	17.32	3.33	6.00e-03	4.77e-02	3TXNRD1, PRNP, GCLM	49
HALLMARK_TNFA_SIGNALING_VIA_NFKB	1.24e-04	8.78	3.02	1.24e-03	6.18e-03	6LIF, CCL2, F3, TIPARP, SERPINE1, PHLDA1	200
HALLMARK_COMPLEMENT	1.08e-03	7.12	2.18	6.00e-03	5.40e-02	5F3, FN1, CFH, SERPINE1, CTSL	200
HALLMARK_INFLAMMATORY_RESPONSE	1.08e-03	7.12	2.18	6.00e-03	5.40e-02	5LIF, CCL2, F3, SERPINE1, GPC3	200
HALLMARK_KRAS_SIGNALING_UP	1.08e-03	7.12	2.18	6.00e-03	5.40e-02	5EMP1, SPRY2, LIF, CFH, TFPI	200
HALLMARK_UV_RESPONSE_DN	2.44e-03	7.76	2.00	1.22e-02	1.22e-01	4F3, SERPINE1, RND3, TFPI	144
HALLMARK_ANGIOGENESIS	8.94e-03	15.30	1.73	3.73e-02	4.47e-01	2LUM, FGFR1	36
HALLMARK_IL2_STAT5_SIGNALING	7.58e-03	5.57	1.44	3.44e-02	3.79e-01	4EMP1, LIF, PHLDA1, PRNP	199
HALLMARK_TGF_BETA_SIGNALING	1.94e-02	10.01	1.15	7.45e-02	9.68e-01	2THBS1, SERPINE1	54
HALLMARK_MYOGENESIS	4.35e-02	4.05	0.80	1.21e-01	1.00e+00	3LAMA2, PRNP, GSN	200
HALLMARK_XENOBIOTIC_METABOLISM	4.35e-02	4.05	0.80	1.21e-01	1.00e+00	3HMOX1, SERPINE1, UGDH	200
HALLMARK_GLYCOLYSIS	4.35e-02	4.05	0.80	1.21e-01	1.00e+00	3DCN, EGFR, GPC3	200
HALLMARK_P53_PATHWAY	4.35e-02	4.05	0.80	1.21e-01	1.00e+00	3LIF, HMOX1, TM4SF1	200
HALLMARK_ALLOGRAFT_REJECTION	4.35e-02	4.05	0.80	1.21e-01	1.00e+00	3LIF, CCL2, EGFR	200
HALLMARK_IL6_JAK_STAT3_SIGNALING	4.64e-02	6.13	0.71	1.22e-01	1.00e+00	2HMOX1, TNFRSF12A	87
HALLMARK_BILE_ACID_METABOLISM	7.22e-02	4.73	0.55	1.81e-01	1.00e+00	2DIO2, GCLM	112

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KEGG Pathways:

	P-value	OR	Lower 95% CI	FDR	FWER	Genes Found	Gene Set Size
KEGG_COMPLEMENT_AND_COAGULATION_CASCADES	1.56e-04	16.71	4.25	2.90e-02	2.90e-02	4F3, CFH, SERPINE1, TFPI	69
KEGG_ECM_RECEPTOR_INTERACTION	4.44e-03	9.85	1.92	2.94e-01	8.25e-01	3THBS1, LAMA2, FN1	84
KEGG_TGF_BETA_SIGNALING_PATHWAY	4.74e-03	9.61	1.88	2.94e-01	8.81e-01	3THBS1, DCN, BMP8B	86
KEGG_BLADDER_CANCER	1.20e-02	13.01	1.48	3.73e-01	1.00e+00	2THBS1, EGFR	42
KEGG_FOCAL_ADHESION	7.58e-03	5.57	1.44	3.52e-01	1.00e+00	4THBS1, EGFR, LAMA2, FN1	199
KEGG_REGULATION_OF_ACTIN_CYTOSKELETON	9.54e-03	5.20	1.35	3.55e-01	1.00e+00	4EGFR, FN1, GSN, FGFR1	213
KEGG_CYTOKINE_CYTOKINE_RECEPTOR_INTERACTION	1.96e-02	4.16	1.08	5.20e-01	1.00e+00	4LIF, CCL2, EGFR, TNFRSF12A	265
KEGG_P53_SIGNALING_PATHWAY	2.97e-02	7.89	0.91	6.26e-01	1.00e+00	2THBS1, SERPINE1	68
KEGG_PATHWAYS_IN_CANCER	3.70e-02	3.39	0.88	6.26e-01	1.00e+00	4EGFR, LAMA2, FN1, FGFR1	325
KEGG_MELANOMA	3.21e-02	7.55	0.87	6.26e-01	1.00e+00	2EGFR, FGFR1	71
KEGG_ADHERENS_JUNCTION	3.38e-02	7.33	0.85	6.26e-01	1.00e+00	2EGFR, FGFR1	73
KEGG_SMALL_CELL_LUNG_CANCER	4.36e-02	6.35	0.74	6.72e-01	1.00e+00	2LAMA2, FN1	84
KEGG_PROSTATE_CANCER	4.83e-02	5.98	0.69	6.72e-01	1.00e+00	2EGFR, FGFR1	89
KEGG_CIRCADIAN_RHYTHM_MAMMAL	5.05e-02	21.22	0.49	6.72e-01	1.00e+00	1BHLHE41	13
KEGG_JAK_STAT_SIGNALING_PATHWAY	1.24e-01	3.40	0.40	1.00e+00	1.00e+00	2SPRY2, LIF	155
KEGG_GLYCOSAMINOGLYCAN_BIOSYNTHESIS_CHONDROITIN_SULFATE	8.40e-02	12.14	0.29	1.00e+00	1.00e+00	1CHSY1	22
KEGG_DORSO_VENTRAL_AXIS_FORMATION	9.13e-02	11.08	0.26	1.00e+00	1.00e+00	1EGFR	24
KEGG_ASCORBATE_AND_ALDARATE_METABOLISM	9.49e-02	10.62	0.25	1.00e+00	1.00e+00	1UGDH	25
KEGG_MAPK_SIGNALING_PATHWAY	2.80e-01	1.97	0.23	1.00e+00	1.00e+00	2EGFR, FGFR1	267
KEGG_PENTOSE_AND_GLUCURONATE_INTERCONVERSIONS	1.06e-01	9.44	0.23	1.00e+00	1.00e+00	1UGDH	28

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CHR Positional Gene Sets:

	P-value	OR	Lower 95% CI	FDR	FWER	Genes Found	Gene Set Size
chr12q21	1.40e-02	6.38	1.25	1.00e+00	1.00e+00	3LUM, DCN, PHLDA1	128
chr3q25	1.20e-01	3.47	0.41	1.00e+00	1.00e+00	2TIPARP, TM4SF1	152
chr9q22	1.64e-01	2.85	0.33	1.00e+00	1.00e+00	2S1PR3, OGN	185
chr9q21	1.68e-01	2.80	0.33	1.00e+00	1.00e+00	2CTSL, ANXA1	188
chr22q12	2.03e-01	2.47	0.29	1.00e+00	1.00e+00	2LIF, HMOX1	213
chr3q28	1.13e-01	8.79	0.21	1.00e+00	1.00e+00	1CLDN1	30
chr5q34	1.41e-01	6.89	0.17	1.00e+00	1.00e+00	1TENM2	38
chr2q23	1.84e-01	5.10	0.12	1.00e+00	1.00e+00	1RND3	51
chr14q31	2.00e-01	4.64	0.11	1.00e+00	1.00e+00	1DIO2	56
chr15q14	2.00e-01	4.64	0.11	1.00e+00	1.00e+00	1THBS1	56
chr10p13	2.25e-01	4.05	0.10	1.00e+00	1.00e+00	1FRMD4A	64
chr4p14	2.25e-01	4.05	0.10	1.00e+00	1.00e+00	1UGDH	64
chr1q31	2.46e-01	3.64	0.09	1.00e+00	1.00e+00	1CFH	71
chr7p11	2.78e-01	3.15	0.08	1.00e+00	1.00e+00	1EGFR	82
chr14q22	3.01e-01	2.87	0.07	1.00e+00	1.00e+00	1FRMD6	90
chr7q32	3.01e-01	2.87	0.07	1.00e+00	1.00e+00	1MEST	90
chr8p11	3.15e-01	2.71	0.07	1.00e+00	1.00e+00	1FGFR1	95
chr13q31	3.20e-01	2.66	0.07	1.00e+00	1.00e+00	1SPRY2	97
chr2p22	3.23e-01	2.63	0.06	1.00e+00	1.00e+00	1CYP1B1	98
chr1p21	3.25e-01	2.60	0.06	1.00e+00	1.00e+00	1F3	99

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Transcription Factor Targets:

	P-value	OR	Lower 95% CI	FDR	FWER	Genes Found	Gene Set Size
P300_01	4.58e-04	6.82	2.35	3.29e-01	5.19e-01	6EMP1, BHLHE41, TNFRSF12A, GSN, FGFR1, GPC3	256
RACCACAR_AML_Q6	5.80e-04	6.51	2.25	3.29e-01	6.58e-01	6EMP1, LIF, CCL2, TNFRSF12A, CFH, FGFR1	268
AHR_Q5	1.08e-02	5.01	1.30	1.00e+00	1.00e+00	4CYP1B1, LUM, BHLHE41, RTN4	221
TTCYNRGAA_STAT5B_01	1.01e-02	4.13	1.27	1.00e+00	1.00e+00	5LIF, CCL2, FRMD6, LAMA2, FN1	341
CDPCR1_01	1.42e-02	6.33	1.24	1.00e+00	1.00e+00	3EMP1, DIO2, BHLHE41	129
POU1F1_Q6	1.42e-02	4.61	1.19	1.00e+00	1.00e+00	4DIO2, TNFRSF12A, RTN4, GPC3	240
IRF_Q6	1.50e-02	4.53	1.17	1.00e+00	1.00e+00	4DIO2, F3, THBS1, RTN4	244
STAT5B_01	1.73e-02	4.33	1.12	1.00e+00	1.00e+00	4LIF, CCL2, FRMD6, LAMA2	255
STAT5A_01	1.82e-02	4.26	1.11	1.00e+00	1.00e+00	4LIF, CCL2, FRMD6, LAMA2	259
DR4_Q2	1.98e-02	4.15	1.08	1.00e+00	1.00e+00	4TNFRSF12A, FRMD4A, OGN, ZBTB21	266
LHX9_TARGET_GENES	2.17e-02	2.27	1.05	1.00e+00	1.00e+00	11TXNRD1, FRMD6, SLC4A7, EGFR, TIPARP, FRMD4A, PHLDA1, PRNP, RTN4, LIMA1, GSN	1563
ATXN7L3_TARGET_GENES	2.36e-02	3.92	1.02	1.00e+00	1.00e+00	4TXNRD1, PRNP, RTN4, UGDH	281
CTGCAGY_UNKNOWN	2.53e-02	2.64	1.00	1.00e+00	1.00e+00	7DIO2, TNFRSF12A, FRMD4A, SERPINE1, RTN4, FGFR1, GPC3	779
PSMB5_TARGET_GENES	3.11e-02	3.59	0.93	1.00e+00	1.00e+00	4EMP1, THBS1, SERPINE1, RND3	307
UBN1_TARGET_GENES	5.07e-02	2.00	0.92	1.00e+00	1.00e+00	11CYP1B1, EMP1, DIO2, F3, THBS1, LAMA2, RTN4, UGDH, GSN, KLHL21, ANXA1	1779
TP73_TARGET_GENES	4.22e-02	2.35	0.89	1.00e+00	1.00e+00	7EMP1, TXNRD1, FRMD6, TIPARP, SERPINE1, LIMA1, PAMR1	875
HES2_TARGET_GENES	9.37e-02	1.95	0.83	1.00e+00	1.00e+00	9HMOX1, DIO2, TXNRD1, SERPINE1, MEST, GSN, FGFR1, TM4SF1, ANXA1	1420
GREB1_TARGET_GENES	8.38e-02	2.18	0.82	1.00e+00	1.00e+00	7CYP1B1, FRMD6, TIPARP, RTN4, GCLM, LIMA1, GSN	941
ZNF776_TARGET_GENES	3.14e-02	36.29	0.79	1.00e+00	1.00e+00	1BHLHE41	8
ZNF514_TARGET_GENES	3.14e-02	36.29	0.79	1.00e+00	1.00e+00	1GSN	8

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GO Biological Processes:

	P-value	OR	Lower 95% CI	FDR	FWER	Genes Found	Gene Set Size
GOBP_NEGATIVE_REGULATION_OF_PLASMINOGEN_ACTIVATION	2.31e-04	129.66	11.45	3.26e-02	1.00e+00	2THBS1, SERPINE1	6
GOBP_POSITIVE_REGULATION_OF_TRANSFORMING_GROWTH_FACTOR_BETA1_PRODUCTION	2.31e-04	129.66	11.45	3.26e-02	1.00e+00	2LUM, THBS1	6
GOBP_PROLACTIN_SECRETION	2.31e-04	129.66	11.45	3.26e-02	1.00e+00	2EGFR, ANXA1	6
GOBP_PEPTIDE_CROSS_LINKING	1.05e-05	34.98	8.63	4.93e-03	7.89e-02	4THBS1, DCN, FN1, ANXA1	35
GOBP_POSITIVE_REGULATION_OF_WOUND_HEALING	3.40e-06	25.67	7.66	3.18e-03	2.55e-02	5F3, THBS1, CLDN1, SERPINE1, ANXA1	59
GOBP_REGULATION_OF_NITRIC_OXIDE_MEDIATED_SIGNAL_TRANSDUCTION	5.49e-04	74.10	7.33	5.96e-02	1.00e+00	2THBS1, EGFR	9
GOBP_POSITIVE_REGULATION_OF_COAGULATION	1.13e-04	37.87	7.01	2.35e-02	8.45e-01	3F3, THBS1, SERPINE1	24
GOBP_CHONDROITIN_SULFATE_BIOSYNTHETIC_PROCESS	1.28e-04	36.22	6.71	2.51e-02	9.57e-01	3DCN, UGDH, CHSY1	25
GOBP_NEGATIVE_REGULATION_OF_FIBRINOLYSIS	6.85e-04	64.86	6.55	6.83e-02	1.00e+00	2THBS1, SERPINE1	10
GOBP_POSITIVE_REGULATION_OF_RESPONSE_TO_WOUNDING	9.11e-06	20.72	6.22	4.76e-03	6.81e-02	5F3, THBS1, CLDN1, SERPINE1, ANXA1	72
GOBP_NEGATIVE_REGULATION_OF_PROTEIN_MATURATION	1.62e-04	33.18	6.19	2.81e-02	1.00e+00	3THBS1, SERPINE1, PRNP	27
GOBP_REGULATION_OF_WOUND_HEALING	7.38e-07	16.40	6.10	1.60e-03	5.52e-03	7F3, THBS1, CLDN1, TNFRSF12A, SERPINE1, TFPI, ANXA1	131
GOBP_PROTEIN_ACTIVATION_CASCADE	1.81e-04	31.85	5.95	2.94e-02	1.00e+00	3F3, FN1, TFPI	28
GOBP_CHONDROITIN_SULFATE_PROTEOGLYCAN_BIOSYNTHETIC_PROCESS	2.22e-04	29.50	5.54	3.26e-02	1.00e+00	3DCN, UGDH, CHSY1	30
GOBP_REGULATION_OF_PHOSPHOLIPASE_A2_ACTIVITY	9.99e-04	51.89	5.39	8.40e-02	1.00e+00	2EGFR, ANXA1	12
GOBP_NEGATIVE_REGULATION_OF_CELL_ADHESION_MEDIATED_BY_INTEGRIN	9.99e-04	51.89	5.39	8.40e-02	1.00e+00	2CYP1B1, SERPINE1	12
GOBP_KERATAN_SULFATE_CATABOLIC_PROCESS	9.99e-04	51.89	5.39	8.40e-02	1.00e+00	2LUM, OGN	12
GOBP_AMINOGLYCAN_BIOSYNTHETIC_PROCESS	5.37e-06	15.77	5.38	3.39e-03	4.02e-02	6LUM, DCN, UGDH, CHSY1, OGN, GPC3	114
GOBP_LIVER_REGENERATION	2.46e-04	28.45	5.35	3.40e-02	1.00e+00	3HMOX1, EGFR, CLDN1	31
GOBP_REGULATION_OF_PLASMINOGEN_ACTIVATION	1.18e-03	47.19	4.96	9.08e-02	1.00e+00	2THBS1, SERPINE1	13

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Immunological Gene Sets:

	P-value	OR	Lower 95% CI	FDR	FWER	Genes Found	Gene Set Size
GSE9988_ANTI_TREM1_VS_CTRL_TREATED_MONOCYTES_UP	3.90e-09	16.62	7.30	1.90e-05	1.90e-05	10SPRY2, F3, TXNRD1, S1PR3, PHLDA1, GCLM, CHSY1, CTSL, KLHL21, ZBTB21	198
GSE14769_UNSTIM_VS_60MIN_LPS_BMDM_DN	9.73e-07	12.40	4.96	1.58e-03	4.74e-03	8SPRY2, F3, FRMD6, CDC42EP2, TIPARP, FRMD4A, PHLDA1, LIMA1	200
GSE24634_TEFF_VS_TCONV_DAY7_IN_CULTURE_DN	9.73e-07	12.40	4.96	1.58e-03	4.74e-03	8CYP1B1, SPRY2, HMOX1, F3, BHLHE41, SERPINE1, GSN, CTSL	200
GSE37605_FOXP3_FUSION_GFP_VS_IRES_GFP_TREG_C57BL6_UP	7.19e-06	11.43	4.28	4.45e-03	3.50e-02	7SPRY2, CCL2, THBS1, EGFR, SERPINE1, RND3, TM4SF1	185
GSE9988_ANTI_TREM1_VS_VEHICLE_TREATED_MONOCYTES_UP	9.76e-06	10.88	4.08	4.45e-03	4.76e-02	7F3, TXNRD1, S1PR3, CHSY1, CTSL, KLHL21, ZBTB21	194
GSE9988_LPS_VS_LPS_AND_ANTI_TREM1_MONOCYTE_DN	9.76e-06	10.88	4.08	4.45e-03	4.76e-02	7SPRY2, S1PR3, TNFRSF12A, SERPINE1, CTSL, KLHL21, ZBTB21	194
GSE9988_ANTI_TREM1_AND_LPS_VS_VEHICLE_TREATED_MONOCYTES_UP	1.01e-05	10.82	4.05	4.45e-03	4.92e-02	7SPRY2, F3, TXNRD1, S1PR3, GCLM, CTSL, KLHL21	195
GSE9988_ANTI_TREM1_VS_LPS_MONOCYTE_UP	1.11e-05	10.65	3.99	4.45e-03	5.42e-02	7EMP1, SPRY2, S1PR3, TNFRSF12A, CHSY1, CTSL, ZBTB21	198
GSE14769_UNSTIM_VS_80MIN_LPS_BMDM_DN	1.15e-05	10.60	3.97	4.45e-03	5.60e-02	7DIO2, FRMD6, CDC42EP2, TIPARP, SERPINE1, PHLDA1, LIMA1	199
GSE45365_NK_CELL_VS_CD11B_DC_DN	1.15e-05	10.60	3.97	4.45e-03	5.60e-02	7LIF, S1PR3, TIPARP, SERPINE1, PHLDA1, CHSY1, TM4SF1	199
GSE22601_IMMATURE_CD4_SINGLE_POSITIVE_VS_CD8_SINGLE_POSITIVE_THYMOCYTE_DN	1.19e-05	10.54	3.95	4.45e-03	5.79e-02	7EMP1, LIF, TXNRD1, FRMD6, TIPARP, PRNP, CHSY1	200
GSE5679_CTRL_VS_PPARG_LIGAND_ROSIGLITAZONE_AND_RARA_AGONIST_AM580_TREATED_DC_DN	1.19e-05	10.54	3.95	4.45e-03	5.79e-02	7EMP1, DIO2, FRMD6, DCN, PRNP, FGFR1, ANXA1	200
GSE22140_HEALTHY_VS_ARTHRITIC_GERMFREE_MOUSE_CD4_TCELL_DN	1.19e-05	10.54	3.95	4.45e-03	5.79e-02	7EMP1, HMOX1, TXNRD1, THBS1, SERPINE1, GCLM, CTSL	200
GSE37605_TREG_VS_TCONV_NOD_FOXP3_FUSION_GFP_DN	5.10e-05	10.39	3.57	1.77e-02	2.48e-01	6CCL2, TXNRD1, THBS1, PHLDA1, RTN4, ANXA1	170
GSE9988_LOW_LPS_VS_ANTI_TREM1_AND_LPS_MONOCYTE_DN	1.11e-04	8.97	3.09	2.08e-02	5.40e-01	6SPRY2, S1PR3, SERPINE1, CHSY1, CTSL, KLHL21	196
GSE17974_0H_VS_1H_IN_VITRO_ACT_CD4_TCELL_DN	1.14e-04	8.92	3.07	2.08e-02	5.55e-01	6LIF, TXNRD1, CLDN1, PHLDA1, CHSY1, CTSL	197
GSE17974_0H_VS_2H_IN_VITRO_ACT_CD4_TCELL_DN	1.17e-04	8.88	3.05	2.08e-02	5.71e-01	6EMP1, LIF, CLDN1, PHLDA1, CHSY1, CTSL	198
GSE24634_TREG_VS_TCONV_POST_DAY7_IL4_CONVERSION_DN	1.17e-04	8.88	3.05	2.08e-02	5.71e-01	6CYP1B1, CCL2, FN1, SERPINE1, GSN, CTSL	198
GSE360_L_DONOVANI_VS_B_MALAYI_HIGH_DOSE_MAC_UP	1.20e-04	8.83	3.04	2.08e-02	5.86e-01	6CYP1B1, SPRY2, HMOX1, TIPARP, SERPINE1, CTSL	199
GSE19401_NAIVE_VS_IMMUNIZED_MOUSE_PLN_FOLLICULAR_DC_UP	1.20e-04	8.83	3.04	2.08e-02	5.86e-01	6EMP1, HMOX1, TXNRD1, THBS1, TIPARP, SERPINE1	199

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Top Ranked Transcription Factors for this Gene Expression Program:

Gene Symbol	TF Rank	DNA Binding Domain	Motif Status	IUPAC PWM	GTEx	DepMap	Decartes
CYP1B1	1	No	Unlikely to be sequence specific TF	Not a DNA binding protein	No motif	None	None
HMOX1	5	No	Unlikely to be sequence specific TF	Not a DNA binding protein	No motif	None	Well characterized enzyme with no additional domains. Included only because GO lists it as a regulator of TFs. No support for DNA binding activity.
BHLHE41	11	Yes	Known motif	Monomer or homomultimer	High-throughput in vitro	None	None
TIPARP	24	No	Unlikely to be sequence specific TF	Not a DNA binding protein	No motif	None	None
PRNP	29	No	Unlikely to be sequence specific TF	Not a DNA binding protein	No motif	None	None
ZBTB21	50	Yes	Likely to be sequence specific TF	Monomer or homomultimer	No motif	None	Virtually nothing is known for this protein except that it has a decent cassette of znfC2H2 domains and a BTB homodimerization domain
TRIB1	53	No	Unlikely to be sequence specific TF	Not a DNA binding protein	No motif	None	Associates with CEBPB and CEBPA and inhibits their activities (PMID: 17724128). This is probably achieved through control of their proteosomal degradation (PMID: 17576771)
EBF2	61	Yes	Known motif	Monomer or homomultimer	In vivo/Misc source	Only known motifs are from Transfac or HocoMoco - origin is uncertain	EBF1 has a nice HT-SELEX model and based on this paper (PMID:23499423). EBF2 binds similar motif to control brown/beige fat cell identity.
FOSL1	63	Yes	Known motif	Monomer or homomultimer	High-throughput in vitro	None	None
NRIP1	65	No	Unlikely to be sequence specific TF	Not a DNA binding protein	No motif	None	Transcriptional cofactor
EGR3	71	Yes	Known motif	Monomer or homomultimer	High-throughput in vitro	None	None
ATF4	74	Yes	Known motif	Monomer or homomultimer	High-throughput in vitro	None	None
ETV1	84	Yes	Known motif	Monomer or homomultimer	High-throughput in vitro	None	None
LARP6	87	No	ssDNA/RNA binding	Not a DNA binding protein	No motif	None	Classic RNA binding protein
FZD1	98	No	Unlikely to be sequence specific TF	Not a DNA binding protein	No motif	None	None
WWTR1	101	No	Unlikely to be sequence specific TF	Not a DNA binding protein	No motif	None	Transcriptional co-activator that binds to TEADs (PMID: 19324877) and TBX5 (PMID: 16332960)
KLF5	111	Yes	Known motif	Monomer or homomultimer	High-throughput in vitro	None	Binds as a monomer and as a dimer (PMID: 25575120).
CEBPB	112	Yes	Known motif	Monomer or homomultimer	High-throughput in vitro	None	None
NR3C1	119	Yes	Known motif	Monomer or homomultimer	High-throughput in vitro	None	None
VGLL3	124	No	Unlikely to be sequence specific TF	Not a DNA binding protein	No motif	None	(PMID: 23380592) describes this protein as a co-activator, and it doesnt seem to have a DBD (fig. 1)

QQ Plot showing correlations with other GEPs in this dataset, calculated by Spearman correlation:

Interactive QQ-plot of gene loadings:

Interactive QQ Plot

A similar QQ-plot as above, but only for instances where the H value is e.g. > 25, i.e. we are confident that the expression program is active above noise. Agreemenet between these binary vectors is tested using the Jaccard Index, with the P-values calculated by an exact test:

Interactive QQ-plot:

Interactive QQ Plot

Singler cell type annotations for the top 50 cells on this program.

Cell ID	Singler label	Singler Delta	Activity Score	Top Singler Raw Scores
WK050-I16	Tissue_stem_cells:BM_MSC:BMP2	0.19	659.69	Raw ScoresFibroblasts:breast: 0.48, iPS_cells:adipose_stem_cells: 0.47, Smooth_muscle_cells:vascular:IL-17: 0.47, Smooth_muscle_cells:vascular: 0.47, iPS_cells:CRL2097_foreskin: 0.47, Tissue_stem_cells:BM_MSC:TGFb3: 0.46, Smooth_muscle_cells:bronchial: 0.46, Chondrocytes:MSC-derived: 0.46, Tissue_stem_cells:BM_MSC:BMP2: 0.46, Osteoblasts: 0.46
WK050-L9	Chondrocytes:MSC-derived	0.19	516.03	Raw ScoresFibroblasts:breast: 0.47, iPS_cells:adipose_stem_cells: 0.47, Tissue_stem_cells:BM_MSC:TGFb3: 0.46, iPS_cells:CRL2097_foreskin: 0.46, Tissue_stem_cells:BM_MSC:BMP2: 0.46, Smooth_muscle_cells:vascular:IL-17: 0.46, Chondrocytes:MSC-derived: 0.46, Smooth_muscle_cells:vascular: 0.46, Osteoblasts: 0.45, Smooth_muscle_cells:bronchial: 0.45
WK050-O22	Tissue_stem_cells:BM_MSC:TGFb3	0.19	502.78	Raw ScoresFibroblasts:breast: 0.46, iPS_cells:CRL2097_foreskin: 0.45, iPS_cells:adipose_stem_cells: 0.45, Smooth_muscle_cells:bronchial: 0.44, Osteoblasts: 0.44, Tissue_stem_cells:BM_MSC:TGFb3: 0.44, Chondrocytes:MSC-derived: 0.44, Smooth_muscle_cells:bronchial:vit_D: 0.44, Tissue_stem_cells:BM_MSC:BMP2: 0.44, Osteoblasts:BMP2: 0.44
WK098-J15	Smooth_muscle_cells:bronchial	0.21	489.86	Raw ScoresSmooth_muscle_cells:bronchial: 0.5, Tissue_stem_cells:BM_MSC:TGFb3: 0.49, Fibroblasts:breast: 0.49, Smooth_muscle_cells:bronchial:vit_D: 0.49, Chondrocytes:MSC-derived: 0.49, iPS_cells:CRL2097_foreskin: 0.49, Osteoblasts: 0.49, Smooth_muscle_cells:vascular: 0.48, Smooth_muscle_cells:vascular:IL-17: 0.48, Tissue_stem_cells:BM_MSC:BMP2: 0.48
WK097-K14	Tissue_stem_cells:BM_MSC:BMP2	0.19	476.13	Raw ScoresFibroblasts:breast: 0.48, Smooth_muscle_cells:bronchial: 0.48, Smooth_muscle_cells:bronchial:vit_D: 0.48, Smooth_muscle_cells:vascular: 0.47, Smooth_muscle_cells:vascular:IL-17: 0.47, iPS_cells:adipose_stem_cells: 0.47, Chondrocytes:MSC-derived: 0.47, Osteoblasts: 0.47, Tissue_stem_cells:BM_MSC:TGFb3: 0.47, Tissue_stem_cells:BM_MSC:BMP2: 0.46
WK098-N8	Tissue_stem_cells:BM_MSC	0.18	473.12	Raw ScoresFibroblasts:breast: 0.51, Smooth_muscle_cells:vascular:IL-17: 0.51, Smooth_muscle_cells:vascular: 0.51, iPS_cells:adipose_stem_cells: 0.5, Osteoblasts: 0.5, iPS_cells:CRL2097_foreskin: 0.5, Smooth_muscle_cells:bronchial: 0.5, Chondrocytes:MSC-derived: 0.5, Tissue_stem_cells:BM_MSC:TGFb3: 0.5, Tissue_stem_cells:BM_MSC:BMP2: 0.5
WK051-F14	Smooth_muscle_cells:bronchial	0.20	453.75	Raw ScoresSmooth_muscle_cells:bronchial: 0.43, Smooth_muscle_cells:bronchial:vit_D: 0.42, Fibroblasts:breast: 0.42, iPS_cells:adipose_stem_cells: 0.41, Tissue_stem_cells:BM_MSC:TGFb3: 0.41, Tissue_stem_cells:BM_MSC:BMP2: 0.41, iPS_cells:CRL2097_foreskin: 0.41, Chondrocytes:MSC-derived: 0.41, Osteoblasts: 0.41, Smooth_muscle_cells:vascular: 0.41
WK097-C15	Chondrocytes:MSC-derived	0.19	453.61	Raw ScoresTissue_stem_cells:BM_MSC:TGFb3: 0.49, Chondrocytes:MSC-derived: 0.49, Fibroblasts:breast: 0.49, Smooth_muscle_cells:bronchial: 0.49, iPS_cells:adipose_stem_cells: 0.49, Smooth_muscle_cells:bronchial:vit_D: 0.49, Osteoblasts: 0.48, Smooth_muscle_cells:vascular:IL-17: 0.48, Tissue_stem_cells:BM_MSC:BMP2: 0.48, Smooth_muscle_cells:vascular: 0.48
WK056-A7	Chondrocytes:MSC-derived	0.19	452.26	Raw ScoresFibroblasts:breast: 0.45, iPS_cells:adipose_stem_cells: 0.44, Smooth_muscle_cells:vascular: 0.44, Smooth_muscle_cells:vascular:IL-17: 0.43, iPS_cells:CRL2097_foreskin: 0.43, Osteoblasts: 0.43, Chondrocytes:MSC-derived: 0.43, Smooth_muscle_cells:bronchial: 0.43, Tissue_stem_cells:BM_MSC:TGFb3: 0.43, Smooth_muscle_cells:bronchial:vit_D: 0.43
WK057-K7	Fibroblasts:breast	0.20	450.64	Raw ScoresFibroblasts:breast: 0.45, iPS_cells:CRL2097_foreskin: 0.44, iPS_cells:adipose_stem_cells: 0.44, Smooth_muscle_cells:vascular:IL-17: 0.43, Smooth_muscle_cells:vascular: 0.43, Osteoblasts: 0.43, Chondrocytes:MSC-derived: 0.43, Smooth_muscle_cells:bronchial: 0.43, Tissue_stem_cells:BM_MSC:TGFb3: 0.43, Smooth_muscle_cells:bronchial:vit_D: 0.43
WK057-I23	Chondrocytes:MSC-derived	0.18	437.44	Raw ScoresFibroblasts:breast: 0.41, iPS_cells:adipose_stem_cells: 0.4, iPS_cells:CRL2097_foreskin: 0.4, Smooth_muscle_cells:vascular:IL-17: 0.39, Osteoblasts: 0.39, Chondrocytes:MSC-derived: 0.39, Smooth_muscle_cells:bronchial: 0.39, Osteoblasts:BMP2: 0.39, Smooth_muscle_cells:vascular: 0.39, Smooth_muscle_cells:bronchial:vit_D: 0.39
WK051-L17	Fibroblasts:breast	0.20	425.04	Raw ScoresFibroblasts:breast: 0.42, iPS_cells:adipose_stem_cells: 0.41, iPS_cells:CRL2097_foreskin: 0.41, Smooth_muscle_cells:bronchial: 0.41, Osteoblasts: 0.4, Smooth_muscle_cells:bronchial:vit_D: 0.4, Chondrocytes:MSC-derived: 0.4, Smooth_muscle_cells:vascular:IL-17: 0.4, Tissue_stem_cells:BM_MSC:TGFb3: 0.4, Smooth_muscle_cells:vascular: 0.4
WK055-I13	Chondrocytes:MSC-derived	0.18	415.91	Raw ScoresFibroblasts:breast: 0.41, iPS_cells:adipose_stem_cells: 0.41, iPS_cells:CRL2097_foreskin: 0.4, Chondrocytes:MSC-derived: 0.4, Tissue_stem_cells:BM_MSC:TGFb3: 0.4, Osteoblasts: 0.4, Smooth_muscle_cells:vascular:IL-17: 0.4, Smooth_muscle_cells:bronchial: 0.4, Tissue_stem_cells:BM_MSC:BMP2: 0.4, Smooth_muscle_cells:bronchial:vit_D: 0.39
WK055-G1	Smooth_muscle_cells:vascular	0.18	415.24	Raw ScoresFibroblasts:breast: 0.45, iPS_cells:adipose_stem_cells: 0.44, Smooth_muscle_cells:vascular:IL-17: 0.43, Smooth_muscle_cells:vascular: 0.43, Tissue_stem_cells:BM_MSC:TGFb3: 0.43, Chondrocytes:MSC-derived: 0.43, iPS_cells:CRL2097_foreskin: 0.43, Smooth_muscle_cells:bronchial: 0.43, Osteoblasts: 0.43, Tissue_stem_cells:BM_MSC:BMP2: 0.43
WK055-I1	Tissue_stem_cells:BM_MSC:BMP2	0.18	412.27	Raw ScoresFibroblasts:breast: 0.43, iPS_cells:adipose_stem_cells: 0.42, Smooth_muscle_cells:bronchial: 0.42, Tissue_stem_cells:BM_MSC:BMP2: 0.41, Chondrocytes:MSC-derived: 0.41, Osteoblasts: 0.41, Smooth_muscle_cells:vascular:IL-17: 0.41, Smooth_muscle_cells:bronchial:vit_D: 0.41, Smooth_muscle_cells:vascular: 0.41, Tissue_stem_cells:BM_MSC:TGFb3: 0.41
WK098-F10	Tissue_stem_cells:BM_MSC:TGFb3	0.17	410.68	Raw ScoresChondrocytes:MSC-derived: 0.46, Fibroblasts:breast: 0.46, iPS_cells:adipose_stem_cells: 0.45, Tissue_stem_cells:BM_MSC:TGFb3: 0.45, Tissue_stem_cells:BM_MSC:BMP2: 0.45, iPS_cells:CRL2097_foreskin: 0.45, Smooth_muscle_cells:vascular:IL-17: 0.45, Osteoblasts: 0.45, Smooth_muscle_cells:bronchial: 0.45, Neurons:Schwann_cell: 0.45
WK055-I14	Smooth_muscle_cells:bronchial	0.20	400.19	Raw ScoresFibroblasts:breast: 0.45, iPS_cells:CRL2097_foreskin: 0.44, Smooth_muscle_cells:bronchial: 0.44, iPS_cells:adipose_stem_cells: 0.44, Smooth_muscle_cells:bronchial:vit_D: 0.43, Osteoblasts: 0.43, Tissue_stem_cells:BM_MSC:TGFb3: 0.43, Smooth_muscle_cells:vascular: 0.43, Smooth_muscle_cells:vascular:IL-17: 0.42, Chondrocytes:MSC-derived: 0.42
WK056-J7	Tissue_stem_cells:BM_MSC:BMP2	0.16	396.09	Raw ScoresFibroblasts:breast: 0.42, iPS_cells:adipose_stem_cells: 0.42, Chondrocytes:MSC-derived: 0.41, iPS_cells:CRL2097_foreskin: 0.41, Smooth_muscle_cells:vascular:IL-17: 0.41, Smooth_muscle_cells:vascular: 0.41, Osteoblasts: 0.41, Tissue_stem_cells:BM_MSC:TGFb3: 0.41, Tissue_stem_cells:BM_MSC:BMP2: 0.4, Osteoblasts:BMP2: 0.4
WK050-F7	Tissue_stem_cells:BM_MSC:TGFb3	0.17	386.49	Raw ScoresFibroblasts:breast: 0.41, Smooth_muscle_cells:vascular:IL-17: 0.4, Tissue_stem_cells:BM_MSC:TGFb3: 0.4, Tissue_stem_cells:BM_MSC:BMP2: 0.4, Chondrocytes:MSC-derived: 0.4, Osteoblasts: 0.4, iPS_cells:adipose_stem_cells: 0.4, Smooth_muscle_cells:vascular: 0.4, Osteoblasts:BMP2: 0.4, iPS_cells:CRL2097_foreskin: 0.39
WK051-A17	iPS_cells:adipose_stem_cells	0.19	385.54	Raw ScoresFibroblasts:breast: 0.43, iPS_cells:adipose_stem_cells: 0.42, iPS_cells:CRL2097_foreskin: 0.42, Smooth_muscle_cells:bronchial: 0.42, Chondrocytes:MSC-derived: 0.42, Smooth_muscle_cells:bronchial:vit_D: 0.42, Osteoblasts: 0.41, Tissue_stem_cells:BM_MSC:TGFb3: 0.41, Smooth_muscle_cells:vascular: 0.41, Smooth_muscle_cells:vascular:IL-17: 0.41
WK055-J15	Tissue_stem_cells:BM_MSC:TGFb3	0.19	376.11	Raw ScoresFibroblasts:breast: 0.43, Smooth_muscle_cells:bronchial: 0.43, iPS_cells:adipose_stem_cells: 0.42, Smooth_muscle_cells:bronchial:vit_D: 0.42, iPS_cells:CRL2097_foreskin: 0.42, Chondrocytes:MSC-derived: 0.42, Osteoblasts: 0.42, Smooth_muscle_cells:vascular: 0.42, Tissue_stem_cells:BM_MSC:TGFb3: 0.42, Tissue_stem_cells:BM_MSC:BMP2: 0.42
WK049-G11	Tissue_stem_cells:BM_MSC:TGFb3	0.17	374.08	Raw ScoresFibroblasts:breast: 0.4, Smooth_muscle_cells:bronchial: 0.4, Smooth_muscle_cells:bronchial:vit_D: 0.4, Smooth_muscle_cells:vascular:IL-17: 0.39, Tissue_stem_cells:BM_MSC:TGFb3: 0.39, iPS_cells:adipose_stem_cells: 0.39, Smooth_muscle_cells:vascular: 0.39, iPS_cells:CRL2097_foreskin: 0.39, Tissue_stem_cells:BM_MSC:BMP2: 0.39, Chondrocytes:MSC-derived: 0.39
WK057-I1	Osteoblasts	0.19	372.30	Raw ScoresFibroblasts:breast: 0.45, Osteoblasts: 0.44, Tissue_stem_cells:BM_MSC:TGFb3: 0.44, iPS_cells:CRL2097_foreskin: 0.44, Smooth_muscle_cells:bronchial: 0.44, iPS_cells:adipose_stem_cells: 0.44, Chondrocytes:MSC-derived: 0.44, Tissue_stem_cells:BM_MSC:BMP2: 0.44, Smooth_muscle_cells:bronchial:vit_D: 0.43, Osteoblasts:BMP2: 0.43
WK057-M15	Tissue_stem_cells:BM_MSC:TGFb3	0.18	365.36	Raw ScoresFibroblasts:breast: 0.4, iPS_cells:adipose_stem_cells: 0.4, Smooth_muscle_cells:bronchial: 0.39, Tissue_stem_cells:BM_MSC:TGFb3: 0.39, Chondrocytes:MSC-derived: 0.39, iPS_cells:CRL2097_foreskin: 0.39, Osteoblasts: 0.39, Smooth_muscle_cells:bronchial:vit_D: 0.39, Smooth_muscle_cells:vascular:IL-17: 0.39, Tissue_stem_cells:BM_MSC:BMP2: 0.39
WK059-I19	Tissue_stem_cells:BM_MSC:BMP2	0.20	364.68	Raw ScoresFibroblasts:breast: 0.48, Smooth_muscle_cells:vascular: 0.47, Osteoblasts: 0.47, Smooth_muscle_cells:vascular:IL-17: 0.47, iPS_cells:CRL2097_foreskin: 0.47, Tissue_stem_cells:BM_MSC:TGFb3: 0.47, iPS_cells:adipose_stem_cells: 0.47, Chondrocytes:MSC-derived: 0.47, Smooth_muscle_cells:bronchial: 0.46, Tissue_stem_cells:BM_MSC:BMP2: 0.46
WK050-I15	Tissue_stem_cells:BM_MSC:TGFb3	0.18	361.39	Raw ScoresFibroblasts:breast: 0.44, Smooth_muscle_cells:vascular:IL-17: 0.43, iPS_cells:adipose_stem_cells: 0.43, Tissue_stem_cells:BM_MSC:TGFb3: 0.43, Smooth_muscle_cells:bronchial: 0.43, Smooth_muscle_cells:vascular: 0.43, Smooth_muscle_cells:bronchial:vit_D: 0.43, Chondrocytes:MSC-derived: 0.43, Tissue_stem_cells:BM_MSC:BMP2: 0.43, iPS_cells:CRL2097_foreskin: 0.43
WK098-C4	Tissue_stem_cells:BM_MSC:TGFb3	0.19	357.06	Raw ScoresFibroblasts:breast: 0.47, Tissue_stem_cells:BM_MSC:TGFb3: 0.46, iPS_cells:CRL2097_foreskin: 0.46, Smooth_muscle_cells:bronchial: 0.46, iPS_cells:adipose_stem_cells: 0.46, Chondrocytes:MSC-derived: 0.46, Smooth_muscle_cells:bronchial:vit_D: 0.46, Tissue_stem_cells:BM_MSC:BMP2: 0.46, Smooth_muscle_cells:vascular:IL-17: 0.46, Osteoblasts: 0.45
WK057-F3	Smooth_muscle_cells:bronchial	0.17	354.65	Raw ScoresFibroblasts:breast: 0.4, iPS_cells:adipose_stem_cells: 0.39, iPS_cells:CRL2097_foreskin: 0.39, Smooth_muscle_cells:bronchial: 0.39, Tissue_stem_cells:BM_MSC:BMP2: 0.39, Chondrocytes:MSC-derived: 0.39, Tissue_stem_cells:BM_MSC:TGFb3: 0.39, Smooth_muscle_cells:vascular:IL-17: 0.39, Smooth_muscle_cells:bronchial:vit_D: 0.39, Smooth_muscle_cells:vascular: 0.38
WK051-H4	Smooth_muscle_cells:bronchial	0.15	352.17	Raw ScoresFibroblasts:breast: 0.37, Smooth_muscle_cells:vascular:IL-17: 0.37, iPS_cells:adipose_stem_cells: 0.37, Smooth_muscle_cells:vascular: 0.37, Smooth_muscle_cells:bronchial: 0.37, Smooth_muscle_cells:bronchial:vit_D: 0.37, Chondrocytes:MSC-derived: 0.37, Tissue_stem_cells:BM_MSC:BMP2: 0.37, iPS_cells:CRL2097_foreskin: 0.36, Tissue_stem_cells:BM_MSC:TGFb3: 0.36
WK059-C5	iPS_cells:adipose_stem_cells	0.20	351.85	Raw ScoresFibroblasts:breast: 0.47, iPS_cells:adipose_stem_cells: 0.46, iPS_cells:CRL2097_foreskin: 0.46, Smooth_muscle_cells:bronchial: 0.46, Smooth_muscle_cells:bronchial:vit_D: 0.45, Smooth_muscle_cells:vascular:IL-17: 0.45, Smooth_muscle_cells:vascular: 0.45, Osteoblasts: 0.45, Chondrocytes:MSC-derived: 0.45, Tissue_stem_cells:BM_MSC:TGFb3: 0.45
WK051-M22	Fibroblasts:breast	0.20	351.64	Raw ScoresFibroblasts:breast: 0.43, Smooth_muscle_cells:vascular:IL-17: 0.42, iPS_cells:adipose_stem_cells: 0.42, Osteoblasts: 0.41, Smooth_muscle_cells:vascular: 0.41, Chondrocytes:MSC-derived: 0.41, Tissue_stem_cells:BM_MSC:TGFb3: 0.41, iPS_cells:CRL2097_foreskin: 0.41, Smooth_muscle_cells:bronchial: 0.41, Smooth_muscle_cells:bronchial:vit_D: 0.41
WK050-A16	Smooth_muscle_cells:bronchial	0.20	349.98	Raw ScoresFibroblasts:breast: 0.46, iPS_cells:CRL2097_foreskin: 0.44, iPS_cells:adipose_stem_cells: 0.44, Smooth_muscle_cells:bronchial: 0.44, Smooth_muscle_cells:bronchial:vit_D: 0.44, Osteoblasts: 0.44, Tissue_stem_cells:BM_MSC:TGFb3: 0.43, Osteoblasts:BMP2: 0.43, Tissue_stem_cells:BM_MSC:BMP2: 0.43, Smooth_muscle_cells:vascular:IL-17: 0.43
WK051-O22	Tissue_stem_cells:BM_MSC:BMP2	0.15	349.09	Raw ScoresiPS_cells:adipose_stem_cells: 0.41, Fibroblasts:breast: 0.41, Tissue_stem_cells:BM_MSC:BMP2: 0.4, Smooth_muscle_cells:bronchial: 0.4, Tissue_stem_cells:BM_MSC:TGFb3: 0.4, iPS_cells:CRL2097_foreskin: 0.4, Smooth_muscle_cells:bronchial:vit_D: 0.4, Chondrocytes:MSC-derived: 0.4, Osteoblasts: 0.4, Smooth_muscle_cells:vascular:IL-17: 0.4
WK049-O21	iPS_cells:CRL2097_foreskin	0.18	347.89	Raw ScoresFibroblasts:breast: 0.41, iPS_cells:CRL2097_foreskin: 0.41, iPS_cells:adipose_stem_cells: 0.4, Smooth_muscle_cells:bronchial: 0.4, Chondrocytes:MSC-derived: 0.4, Tissue_stem_cells:BM_MSC:TGFb3: 0.4, Smooth_muscle_cells:bronchial:vit_D: 0.4, Smooth_muscle_cells:vascular:IL-17: 0.4, Osteoblasts: 0.39, Tissue_stem_cells:BM_MSC:BMP2: 0.39
WK051-F16	Tissue_stem_cells:BM_MSC:BMP2	0.19	338.29	Raw ScoresFibroblasts:breast: 0.44, iPS_cells:adipose_stem_cells: 0.44, iPS_cells:CRL2097_foreskin: 0.43, Smooth_muscle_cells:bronchial: 0.43, Chondrocytes:MSC-derived: 0.43, Tissue_stem_cells:BM_MSC:TGFb3: 0.43, Tissue_stem_cells:BM_MSC:BMP2: 0.43, Osteoblasts: 0.43, Smooth_muscle_cells:bronchial:vit_D: 0.43, Smooth_muscle_cells:vascular:IL-17: 0.43
WK051-H5	Tissue_stem_cells:BM_MSC:TGFb3	0.17	338.01	Raw ScoresFibroblasts:breast: 0.43, iPS_cells:CRL2097_foreskin: 0.43, Smooth_muscle_cells:bronchial: 0.42, Chondrocytes:MSC-derived: 0.42, Smooth_muscle_cells:bronchial:vit_D: 0.42, Osteoblasts: 0.42, Tissue_stem_cells:BM_MSC:TGFb3: 0.42, Smooth_muscle_cells:vascular:IL-17: 0.42, iPS_cells:adipose_stem_cells: 0.42, Smooth_muscle_cells:vascular: 0.42
WK051-M21	Tissue_stem_cells:BM_MSC:BMP2	0.17	330.12	Raw ScoresFibroblasts:breast: 0.44, iPS_cells:CRL2097_foreskin: 0.43, iPS_cells:adipose_stem_cells: 0.42, Chondrocytes:MSC-derived: 0.42, Tissue_stem_cells:BM_MSC:TGFb3: 0.42, Smooth_muscle_cells:vascular:IL-17: 0.42, Tissue_stem_cells:BM_MSC:BMP2: 0.42, Osteoblasts: 0.42, Smooth_muscle_cells:vascular: 0.42, Smooth_muscle_cells:bronchial: 0.42
WK098-H12	Tissue_stem_cells:BM_MSC:BMP2	0.16	329.75	Raw ScoresNeurons:Schwann_cell: 0.43, Fibroblasts:breast: 0.43, Smooth_muscle_cells:vascular:IL-17: 0.43, Chondrocytes:MSC-derived: 0.43, Smooth_muscle_cells:vascular: 0.43, Tissue_stem_cells:BM_MSC:BMP2: 0.42, iPS_cells:adipose_stem_cells: 0.42, Tissue_stem_cells:BM_MSC:TGFb3: 0.42, Smooth_muscle_cells:bronchial: 0.42, iPS_cells:CRL2097_foreskin: 0.42
WK050-F9	Tissue_stem_cells:BM_MSC:TGFb3	0.17	329.17	Raw ScoresFibroblasts:breast: 0.41, iPS_cells:adipose_stem_cells: 0.4, Tissue_stem_cells:BM_MSC:TGFb3: 0.4, iPS_cells:CRL2097_foreskin: 0.4, Smooth_muscle_cells:vascular:IL-17: 0.4, Chondrocytes:MSC-derived: 0.4, Smooth_muscle_cells:bronchial: 0.4, Smooth_muscle_cells:vascular: 0.4, Tissue_stem_cells:BM_MSC:BMP2: 0.4, Osteoblasts: 0.39
WK098-C18	Tissue_stem_cells:BM_MSC:BMP2	0.18	329.11	Raw ScoresFibroblasts:breast: 0.46, iPS_cells:adipose_stem_cells: 0.45, Tissue_stem_cells:BM_MSC:TGFb3: 0.45, Tissue_stem_cells:BM_MSC:BMP2: 0.45, Smooth_muscle_cells:vascular:IL-17: 0.45, Smooth_muscle_cells:bronchial: 0.45, Smooth_muscle_cells:bronchial:vit_D: 0.45, iPS_cells:CRL2097_foreskin: 0.45, Osteoblasts: 0.45, Smooth_muscle_cells:vascular: 0.44
WK051-M17	Tissue_stem_cells:BM_MSC:BMP2	0.17	328.17	Raw ScoresFibroblasts:breast: 0.39, Chondrocytes:MSC-derived: 0.38, Tissue_stem_cells:BM_MSC:TGFb3: 0.38, iPS_cells:CRL2097_foreskin: 0.38, Tissue_stem_cells:BM_MSC:BMP2: 0.38, Smooth_muscle_cells:vascular:IL-17: 0.38, Smooth_muscle_cells:bronchial: 0.38, Osteoblasts: 0.38, iPS_cells:adipose_stem_cells: 0.38, Smooth_muscle_cells:bronchial:vit_D: 0.38
WK057-L11	Tissue_stem_cells:BM_MSC:BMP2	0.16	322.10	Raw ScoresFibroblasts:breast: 0.39, Smooth_muscle_cells:vascular:IL-17: 0.38, iPS_cells:adipose_stem_cells: 0.38, Smooth_muscle_cells:vascular: 0.38, Osteoblasts: 0.38, Chondrocytes:MSC-derived: 0.38, iPS_cells:CRL2097_foreskin: 0.38, Tissue_stem_cells:BM_MSC:BMP2: 0.37, Tissue_stem_cells:BM_MSC:TGFb3: 0.37, Osteoblasts:BMP2: 0.37
WK056-G1	Tissue_stem_cells:BM_MSC:TGFb3	0.16	319.55	Raw ScoresFibroblasts:breast: 0.42, Smooth_muscle_cells:vascular:IL-17: 0.42, iPS_cells:CRL2097_foreskin: 0.42, Chondrocytes:MSC-derived: 0.42, Tissue_stem_cells:BM_MSC:TGFb3: 0.42, iPS_cells:adipose_stem_cells: 0.42, Smooth_muscle_cells:vascular: 0.41, Tissue_stem_cells:BM_MSC:BMP2: 0.41, Smooth_muscle_cells:bronchial: 0.41, Smooth_muscle_cells:bronchial:vit_D: 0.41
WK055-F18	Tissue_stem_cells:BM_MSC:BMP2	0.17	315.81	Raw ScoresTissue_stem_cells:BM_MSC:TGFb3: 0.39, Fibroblasts:breast: 0.39, Smooth_muscle_cells:vascular:IL-17: 0.39, Chondrocytes:MSC-derived: 0.39, Tissue_stem_cells:BM_MSC:BMP2: 0.39, iPS_cells:CRL2097_foreskin: 0.38, Osteoblasts: 0.38, Smooth_muscle_cells:vascular: 0.38, iPS_cells:adipose_stem_cells: 0.38, Smooth_muscle_cells:bronchial: 0.38
WK055-O4	Tissue_stem_cells:BM_MSC:TGFb3	0.17	312.71	Raw ScoresFibroblasts:breast: 0.45, iPS_cells:adipose_stem_cells: 0.44, Smooth_muscle_cells:bronchial: 0.43, Tissue_stem_cells:BM_MSC:TGFb3: 0.43, Chondrocytes:MSC-derived: 0.43, Smooth_muscle_cells:bronchial:vit_D: 0.43, Smooth_muscle_cells:vascular: 0.43, Smooth_muscle_cells:vascular:IL-17: 0.43, Osteoblasts: 0.43, iPS_cells:CRL2097_foreskin: 0.43
WK101-J15	Tissue_stem_cells:BM_MSC:BMP2	0.18	310.62	Raw ScoresFibroblasts:breast: 0.45, Smooth_muscle_cells:bronchial: 0.45, iPS_cells:adipose_stem_cells: 0.44, Chondrocytes:MSC-derived: 0.44, iPS_cells:CRL2097_foreskin: 0.44, Smooth_muscle_cells:bronchial:vit_D: 0.44, Osteoblasts: 0.44, Smooth_muscle_cells:vascular:IL-17: 0.44, Tissue_stem_cells:BM_MSC:TGFb3: 0.44, Smooth_muscle_cells:vascular: 0.43
WK051-N9	Fibroblasts:breast	0.19	309.23	Raw ScoresFibroblasts:breast: 0.41, iPS_cells:CRL2097_foreskin: 0.41, Smooth_muscle_cells:vascular:IL-17: 0.41, iPS_cells:adipose_stem_cells: 0.4, Chondrocytes:MSC-derived: 0.4, Smooth_muscle_cells:vascular: 0.4, Osteoblasts: 0.39, Tissue_stem_cells:BM_MSC:TGFb3: 0.39, Smooth_muscle_cells:bronchial: 0.39, Osteoblasts:BMP2: 0.39
WK097-B15	Neurons:Schwann_cell	0.14	305.85	Raw ScoresFibroblasts:breast: 0.46, Chondrocytes:MSC-derived: 0.45, iPS_cells:adipose_stem_cells: 0.45, Smooth_muscle_cells:bronchial: 0.45, Tissue_stem_cells:BM_MSC:BMP2: 0.45, iPS_cells:CRL2097_foreskin: 0.45, Osteoblasts: 0.45, Smooth_muscle_cells:vascular: 0.45, Smooth_muscle_cells:vascular:IL-17: 0.45, Smooth_muscle_cells:bronchial:vit_D: 0.45
WK098-O19	Neurons:Schwann_cell	0.18	298.54	Raw ScoresFibroblasts:breast: 0.45, iPS_cells:adipose_stem_cells: 0.44, Osteoblasts: 0.44, Chondrocytes:MSC-derived: 0.44, iPS_cells:CRL2097_foreskin: 0.44, Smooth_muscle_cells:bronchial: 0.43, Tissue_stem_cells:BM_MSC:TGFb3: 0.43, Osteoblasts:BMP2: 0.43, Smooth_muscle_cells:vascular:IL-17: 0.43, Smooth_muscle_cells:vascular: 0.43
WK055-D14	Osteoblasts	0.20	296.23	Raw ScoresFibroblasts:breast: 0.44, Smooth_muscle_cells:bronchial: 0.43, iPS_cells:adipose_stem_cells: 0.43, iPS_cells:CRL2097_foreskin: 0.43, Smooth_muscle_cells:bronchial:vit_D: 0.42, Chondrocytes:MSC-derived: 0.42, Osteoblasts: 0.42, Tissue_stem_cells:BM_MSC:TGFb3: 0.42, Osteoblasts:BMP2: 0.42, Tissue_stem_cells:BM_MSC:BMP2: 0.41

Below shows the significant enrichments of this GEP for literature curated gene lists
This data was procured from existing single cell RNA-seq maps of neuroblastoma or related relevant data.
High ranks indicate this gene is a driver of this GEP.
These curated gene list are ranked by the P-value (on this GEP) of their constituent genes.
The Mean Count column shows the mean read count in cells scoring highly (H > 50) on this gene expression program.

Mesenchymal (Olsen)
Markers listed in on page 3 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are stated as being expressed in a group of mesenchymal-like cells (some at least claimed to be neuroblastoma cells) shown in their UMAP representation on Fig. 1D. They reference the two Nature Genetics paper.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.46e-03
Mean rank of genes in gene set: 1539.25
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
DCN	0.0066557	18	GTEx	DepMap	Descartes	65.98	2707.49
PRRX1	0.0027156	236	GTEx	DepMap	Descartes	2.75	204.10
PDGFRA	0.0007088	1311	GTEx	DepMap	Descartes	1.19	45.86
LEPR	-0.0000623	4592	GTEx	DepMap	Descartes	0.29	10.39

Inflammatory CAF
These marker genes were curated across cancer subtypes in multiple organ systems as reviewed in Lavie et. al. (PMID 35883004) and contain inflammatory specific CAF genes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.76e-03
Mean rank of genes in gene set: 4487.2
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LIF	0.0086612	4	GTEx	DepMap	Descartes	15.10	1147.66
CCL2	0.0080405	7	GTEx	DepMap	Descartes	69.43	21560.64
SCARA5	0.0029524	197	GTEx	DepMap	Descartes	1.54	111.27
HGF	0.0026565	242	GTEx	DepMap	Descartes	1.02	47.26
SCARA3	0.0023619	292	GTEx	DepMap	Descartes	1.45	118.87
IGFBP6	0.0019957	398	GTEx	DepMap	Descartes	1.43	319.58
PDGFRB	0.0018848	429	GTEx	DepMap	Descartes	2.88	133.61
SERPING1	0.0017595	482	GTEx	DepMap	Descartes	7.54	871.53
IGF1	0.0017428	491	GTEx	DepMap	Descartes	3.75	109.48
PDPN	0.0013694	667	GTEx	DepMap	Descartes	0.91	65.50
CFB	0.0008895	1082	GTEx	DepMap	Descartes	0.12	8.99
PDGFRA	0.0007088	1311	GTEx	DepMap	Descartes	1.19	45.86
CXCL12	0.0006585	1408	GTEx	DepMap	Descartes	3.73	288.17
PDGFD	0.0004320	1913	GTEx	DepMap	Descartes	0.62	38.64
SOD2	0.0003152	2256	GTEx	DepMap	Descartes	12.67	203.88
RGMA	0.0000388	3634	GTEx	DepMap	Descartes	0.13	3.14
IL1R1	-0.0001073	5262	GTEx	DepMap	Descartes	1.71	72.27
CXCL2	-0.0002452	7538	GTEx	DepMap	Descartes	10.76	2079.77
IL10	-0.0005270	10749	GTEx	DepMap	Descartes	0.08	6.62
IL33	-0.0006483	11425	GTEx	DepMap	Descartes	0.36	31.43
CFD	-0.0011825	12397	GTEx	DepMap	Descartes	0.69	132.73
GPX3	-0.0012700	12432	GTEx	DepMap	Descartes	0.36	34.50
C3	-0.0014669	12486	GTEx	DepMap	Descartes	4.57	181.77
CXCL14	-0.0019523	12535	GTEx	DepMap	Descartes	0.56	69.75
C7	-0.0020766	12543	GTEx	DepMap	Descartes	3.67	123.93

Bridge region mesencchymal (Olsen)
Marker genes shown in Fig. 3A of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - Cells were ordered by pseudotime in the bridge region, this first group of cells were from the mesenchymal part of the trajectory.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.26e-03
Mean rank of genes in gene set: 3909.92
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LUM	0.0079736	8	GTEx	DepMap	Descartes	48.81	4979.99
MAFF	0.0031030	173	GTEx	DepMap	Descartes	6.77	607.17
PRRX1	0.0027156	236	GTEx	DepMap	Descartes	2.75	204.10
MYC	0.0018451	453	GTEx	DepMap	Descartes	2.85	240.71
CALD1	0.0013753	660	GTEx	DepMap	Descartes	6.78	352.44
FGF1	0.0008655	1112	GTEx	DepMap	Descartes	0.15	10.34
PDGFRA	0.0007088	1311	GTEx	DepMap	Descartes	1.19	45.86
KLF4	0.0006225	1478	GTEx	DepMap	Descartes	4.80	494.47
COL1A2	0.0000118	3867	GTEx	DepMap	Descartes	31.50	1208.43
TNNT2	-0.0002055	6889	GTEx	DepMap	Descartes	0.01	0.39
NPNT	-0.0004989	10536	GTEx	DepMap	Descartes	0.05	1.60
PODXL	-0.0006895	11573	GTEx	DepMap	Descartes	0.11	2.57
BGN	-0.0018968	12533	GTEx	DepMap	Descartes	3.72	283.29

Stromal 2 (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Stroma subcluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.16e-02
Mean rank of genes in gene set: 3901.92
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GSN	0.0055647	35	GTEx	DepMap	Descartes	22.16	1025.64
FBN2	0.0048333	54	GTEx	DepMap	Descartes	0.83	20.71
FSTL1	0.0033401	145	GTEx	DepMap	Descartes	8.29	366.68
FBN1	0.0033118	150	GTEx	DepMap	Descartes	4.51	97.71
SFRP1	0.0007126	1308	GTEx	DepMap	Descartes	1.78	109.51
IGFBP5	0.0005632	1583	GTEx	DepMap	Descartes	12.09	460.36
ITM2A	0.0005227	1671	GTEx	DepMap	Descartes	2.45	436.91
COL5A2	0.0001650	2880	GTEx	DepMap	Descartes	2.22	75.32
PENK	0.0000682	3409	GTEx	DepMap	Descartes	0.27	59.96
POSTN	-0.0005573	10943	GTEx	DepMap	Descartes	1.42	72.90
GAS1	-0.0009070	12124	GTEx	DepMap	Descartes	0.10	9.77
COL14A1	-0.0017143	12521	GTEx	DepMap	Descartes	2.08	48.33

EMT II (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this EMT program was enriched in HNSCC cell lines:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.63e-02
Mean rank of genes in gene set: 2405.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FN1	0.0064211	22	GTEx	DepMap	Descartes	26.36	784.78
VIM	0.0019695	405	GTEx	DepMap	Descartes	47.43	5041.69
AXL	0.0018825	431	GTEx	DepMap	Descartes	1.87	111.22
COL5A1	-0.0003333	8764	GTEx	DepMap	Descartes	2.94	76.84

Stress response (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.05e-02
Mean rank of genes in gene set: 2966
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GADD45A	0.0033973	139	GTEx	DepMap	Descartes	6.01	1331.78
SQSTM1	0.0030514	180	GTEx	DepMap	Descartes	12.19	1089.48
DDIT3	0.0015366	577	GTEx	DepMap	Descartes	1.11	298.55
ATF3	0.0006564	1412	GTEx	DepMap	Descartes	7.34	981.35
GADD45B	-0.0017347	12522	GTEx	DepMap	Descartes	2.86	390.97

p53 Dependent Senescence (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.90e-02
Mean rank of genes in gene set: 1419.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CDKN1A	0.0019265	418	GTEx	DepMap	Descartes	13.78	1608.28
NEAT1	0.0002688	2421	GTEx	DepMap	Descartes	34.42	423.38

Protein maturation (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.00e-02
Mean rank of genes in gene set: 2342.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PDIA3	0.0013978	648	GTEx	DepMap	Descartes	2.50	176.70
RPN2	0.0002028	2687	GTEx	DepMap	Descartes	1.29	140.03
OS9	0.0000305	3693	GTEx	DepMap	Descartes	1.42	91.46

Subepicardial mesenchyme (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.19e-02
Mean rank of genes in gene set: 3907.86
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PRRX1	0.0027156	236	GTEx	DepMap	Descartes	2.75	204.10
TWIST2	0.0024746	274	GTEx	DepMap	Descartes	1.06	211.07
TWIST1	0.0023214	306	GTEx	DepMap	Descartes	1.30	201.07
TBX18	0.0018608	441	GTEx	DepMap	Descartes	0.66	29.45
COL1A2	0.0000118	3867	GTEx	DepMap	Descartes	31.50	1208.43
COL3A1	-0.0005424	10850	GTEx	DepMap	Descartes	30.42	1123.54
COL1A1	-0.0006378	11381	GTEx	DepMap	Descartes	56.71	2008.04

Inflammatory Macrophages (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.75e-02
Mean rank of genes in gene set: 228
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
VCAN	0.0027457	228	GTEx	DepMap	Descartes	8.44	194.54

Mixed phenotype identified at the fate split point mouse (olsen)
Stated on page 6 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - seems to be referencing previous mouse lineage tracing studies in mouse which sucggested SCPs give rise to autonomic neurons and mesenchymal cells. markers are of the split point where SCPs differentiate.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.78e-02
Mean rank of genes in gene set: 236
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PRRX1	0.0027156	236	GTEx	DepMap	Descartes	2.75	204.1

Endothelium (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the broad Endothelium cluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.87e-02
Mean rank of genes in gene set: 269
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SPARCL1	0.0025169	269	GTEx	DepMap	Descartes	6.99	610.8

Mesenchymal (Kildisiute)
Mesenchymal markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.33e-02
Mean rank of genes in gene set: 429
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PDGFRB	0.0018848	429	GTEx	DepMap	Descartes	2.88	133.61

Mesemchymal validated (Olsen)
Stated on pages 7 and 8 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - it is stated that a mesenchymal-like neuroblastoma cell state is validate by combined immunofluorescence staining for PDGFRA and FISH for PPMID gain, a gene on 17q.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.53e-02
Mean rank of genes in gene set: 1311
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PDGFRA	0.0007088	1311	GTEx	DepMap	Descartes	1.19	45.86

Melanocytes (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.02e-01
Mean rank of genes in gene set: 3624
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MITF	0.0008705	1107	GTEx	DepMap	Descartes	0.41	23.79
DCT	0.0000172	3809	GTEx	DepMap	Descartes	0.01	0.47
PMEL	-0.0001499	5956	GTEx	DepMap	Descartes	0.01	0.48

Stroma (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the broad Stroma cluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.12e-01
Mean rank of genes in gene set: 4306.4
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FN1	0.0064211	22	GTEx	DepMap	Descartes	26.36	784.78
GPC3	0.0050450	47	GTEx	DepMap	Descartes	4.20	536.32
COL6A1	0.0004530	1849	GTEx	DepMap	Descartes	8.94	527.38
COL5A1	-0.0003333	8764	GTEx	DepMap	Descartes	2.94	76.84
COL3A1	-0.0005424	10850	GTEx	DepMap	Descartes	30.42	1123.54

Mesenchymal cells (Yuan)
Obtained from Fig. 1 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.31e-01
Mean rank of genes in gene set: 4242.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
VIM	0.0019695	405	GTEx	DepMap	Descartes	47.43	5041.69
COL6A1	0.0004530	1849	GTEx	DepMap	Descartes	8.94	527.38
COL1A2	0.0000118	3867	GTEx	DepMap	Descartes	31.50	1208.43
COL3A1	-0.0005424	10850	GTEx	DepMap	Descartes	30.42	1123.54

Pigmentation (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, Skin pigmentation genes, identified in melanoma.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.36e-01
Mean rank of genes in gene set: 4288
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MITF	0.0008705	1107	GTEx	DepMap	Descartes	0.41	23.79
DCT	0.0000172	3809	GTEx	DepMap	Descartes	0.01	0.47
PMEL	-0.0001499	5956	GTEx	DepMap	Descartes	0.01	0.48
MLANA	-0.0001689	6280	GTEx	DepMap	Descartes	0.00	0.00

SCPs neuroblastoma unique (Olsen)
Similar to above, but for genes unique to neuroblastoma SCP-like cells. Note FN1 is often used as a mesenchymal marker gene.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.50e-01
Mean rank of genes in gene set: 4743.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FN1	0.0064211	22	GTEx	DepMap	Descartes	26.36	784.78
ANXA1	0.0049648	49	GTEx	DepMap	Descartes	19.94	2433.59
TIMP3	0.0011308	842	GTEx	DepMap	Descartes	10.08	587.31
GFRA3	-0.0001294	5630	GTEx	DepMap	Descartes	0.23	33.89
ITGB8	-0.0004240	9859	GTEx	DepMap	Descartes	0.72	20.76
IFITM3	-0.0008710	12060	GTEx	DepMap	Descartes	14.06	4764.09

Erythrocytes (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that HBA2+ Erythrocytes were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.55e-01
Mean rank of genes in gene set: 2602
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ANK1	0.0002225	2602	GTEx	DepMap	Descartes	0.16	4.02

Adrenal Premordium (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Adrenal Premordium subcluster, which is part of the Cortex cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.97e-01
Mean rank of genes in gene set: 4732.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MIF	0.0002216	2605	GTEx	DepMap	Descartes	0.30	81.35
TPI1	0.0002124	2644	GTEx	DepMap	Descartes	3.04	460.26
NPM1	0.0002007	2697	GTEx	DepMap	Descartes	6.39	926.10
TK1	-0.0005628	10985	GTEx	DepMap	Descartes	0.02	1.44

Mesenchymal Fig 1D (Olsen)
Selected mesenchymal marker genes shown in Fig. 1D of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these are highly expressed in their mesenchymal cluster on their UMAP.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.04e-01
Mean rank of genes in gene set: 5442.54
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LUM	0.0079736	8	GTEx	DepMap	Descartes	48.81	4979.99
DCN	0.0066557	18	GTEx	DepMap	Descartes	65.98	2707.49
PRRX1	0.0027156	236	GTEx	DepMap	Descartes	2.75	204.10
COL6A2	0.0023295	305	GTEx	DepMap	Descartes	10.72	778.75
CALD1	0.0013753	660	GTEx	DepMap	Descartes	6.78	352.44
PDGFRA	0.0007088	1311	GTEx	DepMap	Descartes	1.19	45.86
COL1A2	0.0000118	3867	GTEx	DepMap	Descartes	31.50	1208.43
LEPR	-0.0000623	4592	GTEx	DepMap	Descartes	0.29	10.39
COL3A1	-0.0005424	10850	GTEx	DepMap	Descartes	30.42	1123.54
COL1A1	-0.0006378	11381	GTEx	DepMap	Descartes	56.71	2008.04
SPARC	-0.0013402	12452	GTEx	DepMap	Descartes	18.88	1082.86
BGN	-0.0018968	12533	GTEx	DepMap	Descartes	3.72	283.29
MGP	-0.0020041	12540	GTEx	DepMap	Descartes	10.52	1172.57

Endothelial subclusters 2-6 (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Various endothelial subclusters of Endothelium clusters:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.63e-01
Mean rank of genes in gene set: 5760.7
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
TM4SF1	0.0050563	46	GTEx	DepMap	Descartes	19.49	1721.22
FBLN2	0.0028102	217	GTEx	DepMap	Descartes	4.07	265.25
CLIC4	0.0022368	325	GTEx	DepMap	Descartes	3.24	196.89
DUSP6	0.0020967	375	GTEx	DepMap	Descartes	3.84	238.93
THBD	0.0011360	835	GTEx	DepMap	Descartes	2.29	131.91
SHROOM2	0.0007913	1191	GTEx	DepMap	Descartes	0.12	4.20
ADAMTS1	0.0007476	1251	GTEx	DepMap	Descartes	4.44	256.99
JAG1	0.0004347	1903	GTEx	DepMap	Descartes	0.81	32.18
MARCKSL1	0.0001337	3021	GTEx	DepMap	Descartes	1.77	307.35
IGFBP3	0.0000810	3320	GTEx	DepMap	Descartes	1.25	106.10
GJA1	0.0000318	3682	GTEx	DepMap	Descartes	0.97	62.95
RHOB	-0.0002767	7986	GTEx	DepMap	Descartes	2.44	273.28
MECOM	-0.0005094	10610	GTEx	DepMap	Descartes	0.03	0.62
IVNS1ABP	-0.0005640	10996	GTEx	DepMap	Descartes	1.50	101.63
CDK1	-0.0005752	11058	GTEx	DepMap	Descartes	0.02	1.42
LFNG	-0.0006067	11222	GTEx	DepMap	Descartes	0.04	3.70
SPRY1	-0.0007229	11679	GTEx	DepMap	Descartes	0.72	63.44
MCF2L	-0.0007257	11686	GTEx	DepMap	Descartes	0.03	0.84
HES1	-0.0007954	11880	GTEx	DepMap	Descartes	2.88	519.69
FAM102A	-0.0008179	11931	GTEx	DepMap	Descartes	0.44	26.87

Liver Primordium (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.75e-01
Mean rank of genes in gene set: 4745
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
VTN	-0.0000263	4204	GTEx	DepMap	Descartes	0	0.6
AHSG	-0.0001088	5286	GTEx	DepMap	Descartes	0	0.0

Mesenchymal cells (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that COL1A1+ Mesenchymal were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.08e-01
Mean rank of genes in gene set: 5225.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PDGFRB	0.0018848	429	GTEx	DepMap	Descartes	2.88	133.61
COL1A2	0.0000118	3867	GTEx	DepMap	Descartes	31.50	1208.43
COL1A1	-0.0006378	11381	GTEx	DepMap	Descartes	56.71	2008.04

Bridge (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.79e-01
Mean rank of genes in gene set: 5629
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CTTNBP2	0.0003555	2134	GTEx	DepMap	Descartes	0.04	1.90
CDH9	-0.0001551	6043	GTEx	DepMap	Descartes	0.00	0.00
ERBB4	-0.0003287	8710	GTEx	DepMap	Descartes	0.00	0.04

Pre-E Chromafin (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Pre Epinepherine Chromaffin subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.01e-01
Mean rank of genes in gene set: 6011.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ARHGEF28	0.0024610	278	GTEx	DepMap	Descartes	0.75	NA
SLC31A1	0.0008867	1085	GTEx	DepMap	Descartes	0.40	23.90
LRP11	0.0001274	3059	GTEx	DepMap	Descartes	0.20	14.52
PCP4	0.0000025	3947	GTEx	DepMap	Descartes	0.02	NA
SLC38A4	-0.0000056	4009	GTEx	DepMap	Descartes	0.01	0.56
TLE1	-0.0001611	6153	GTEx	DepMap	Descartes	0.54	33.83
ARNT2	-0.0002147	7049	GTEx	DepMap	Descartes	0.04	1.99
FAM163A	-0.0002920	8205	GTEx	DepMap	Descartes	0.01	0.42
RPS6KA6	-0.0003124	8495	GTEx	DepMap	Descartes	0.04	1.37
TMX4	-0.0003699	9223	GTEx	DepMap	Descartes	0.82	37.81
NELFCD	-0.0004002	9607	GTEx	DepMap	Descartes	0.40	NA
JUN	-0.0005701	11028	GTEx	DepMap	Descartes	9.03	790.91

Proteasomal degradation (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this is for proteasomal degradataion:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.04e-01
Mean rank of genes in gene set: 5883.2
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PSMA3	0.0005148	1688	GTEx	DepMap	Descartes	0.94	199.93
PSMC2	0.0003344	2194	GTEx	DepMap	Descartes	0.67	59.16
PSME2	-0.0002267	7252	GTEx	DepMap	Descartes	0.81	76.62
PSMA4	-0.0003024	8371	GTEx	DepMap	Descartes	0.44	23.68
PSMB3	-0.0004290	9911	GTEx	DepMap	Descartes	0.49	162.87

Erythroid cells (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.38e-01
Mean rank of genes in gene set: 5705
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ALAS2	-0.0001333	5705	GTEx	DepMap	Descartes	0	0

Mesencchymal populations mouse (Olsen)
Stated on page 6 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - seems to be referencing previous mouse lineage tracing studies in mouse which sucggested SCPs give rise to autonomic neurons and mesenchymal cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.66e-01
Mean rank of genes in gene set: 6056
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PRRX1	0.0027156	236	GTEx	DepMap	Descartes	2.75	204.10
FLI1	-0.0007937	11876	GTEx	DepMap	Descartes	0.08	2.55

Adrenal cortex (Kildisiute)
Adrenal cortex markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.74e-01
Mean rank of genes in gene set: 6042
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
STAR	-0.0001551	6042	GTEx	DepMap	Descartes	0.01	0.64

Adrenal gland cortex (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. :
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.74e-01
Mean rank of genes in gene set: 6042
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
STAR	-0.0001551	6042	GTEx	DepMap	Descartes	0.01	0.64

Angioblast (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Angioblast subcluster of Endothelium cluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.95e-01
Mean rank of genes in gene set: 6249.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
NES	0.0007545	1242	GTEx	DepMap	Descartes	1.29	63.86
MAP4K4	-0.0001183	5451	GTEx	DepMap	Descartes	1.60	55.91
PREX1	-0.0008695	12055	GTEx	DepMap	Descartes	0.15	5.63

SCPs mouse unique (Olsen)
Selected list of SCP marker genes shown in Fig. 2B of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these genes were unique to mouse SCPs as determined by the linearge tracing study Furlan et al. (Science 2017, PMID 28684471).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.99e-01
Mean rank of genes in gene set: 6270.25
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SCRG1	0.0015786	558	GTEx	DepMap	Descartes	0.33	20.73
H1FX	-0.0000977	5106	GTEx	DepMap	Descartes	1.75	NA
CRABP1	-0.0003108	8474	GTEx	DepMap	Descartes	0.01	1.65
POSTN	-0.0005573	10943	GTEx	DepMap	Descartes	1.42	72.90

Muscle progenitor (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that PAX7+ Muscle progenitor were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.01e-01
Mean rank of genes in gene set: 6281.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CDON	-0.0001346	5723	GTEx	DepMap	Descartes	0.11	3.3
PAX7	-0.0002027	6840	GTEx	DepMap	Descartes	0.00	0.0

IFN Response (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this program contained interferon response genes.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.03e-01
Mean rank of genes in gene set: 6288.4
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IFIT1	0.0011894	796	GTEx	DepMap	Descartes	0.50	28.59
IFIT3	0.0002602	2448	GTEx	DepMap	Descartes	0.51	56.28
IFIT2	-0.0000640	4621	GTEx	DepMap	Descartes	0.11	7.12
ISG15	-0.0007139	11650	GTEx	DepMap	Descartes	0.45	124.33
ISG20	-0.0008138	11927	GTEx	DepMap	Descartes	0.19	8.30

Hepatocytes (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that ALB+ Hepatocytes were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D. The main text also speculated that these hepatocytes were probably derived from neighboring tissue, and hence artifactual, but there is no evidence presented for this.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.34e-01
Mean rank of genes in gene set: 6491.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ALB	-0.0000905	4991	GTEx	DepMap	Descartes	0.01	1.18
ASGR1	-0.0002771	7992	GTEx	DepMap	Descartes	0.10	13.21

Schwann cell Precursor (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the SCP subcluster, which is part of the Adrenal Medulla cluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.54e-01
Mean rank of genes in gene set: 6706
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LMO4	0.0015843	552	GTEx	DepMap	Descartes	3.22	186.04
CST3	0.0014059	642	GTEx	DepMap	Descartes	12.58	935.19
NGFR	0.0007967	1182	GTEx	DepMap	Descartes	2.77	225.90
DAGLA	0.0000154	3833	GTEx	DepMap	Descartes	0.05	1.86
CNP	-0.0000658	4648	GTEx	DepMap	Descartes	0.91	45.38
PLP1	-0.0003478	8954	GTEx	DepMap	Descartes	0.52	44.00
SERPINE2	-0.0003903	9493	GTEx	DepMap	Descartes	1.24	46.02
TTYH1	-0.0004474	10085	GTEx	DepMap	Descartes	0.01	0.61
PTPRZ1	-0.0005980	11175	GTEx	DepMap	Descartes	0.04	0.95
ERBB3	-0.0006584	11457	GTEx	DepMap	Descartes	0.04	1.80
CHL1	-0.0007457	11745	GTEx	DepMap	Descartes	0.21	5.52

EpiSen (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, an epithelial senescence associated program.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.78e-01
Mean rank of genes in gene set: 7113
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LCN2	-0.0000362	4292	GTEx	DepMap	Descartes	0.00	0.53
CLDN4	-0.0000907	4995	GTEx	DepMap	Descartes	0.00	0.33
SLPI	-0.0003117	8489	GTEx	DepMap	Descartes	0.00	0.00
AQP3	-0.0005170	10676	GTEx	DepMap	Descartes	0.08	5.55

Neuroblastoma SCPs but not normal SCPs (Olsen)
The same as above:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.17e-01
Mean rank of genes in gene set: 7744.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GFRA3	-0.0001294	5630	GTEx	DepMap	Descartes	0.23	33.89
ITGB8	-0.0004240	9859	GTEx	DepMap	Descartes	0.72	20.76

Endothelial Cells (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.52e-01
Mean rank of genes in gene set: 8017.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ENG	-5.16e-05	4458	GTEx	DepMap	Descartes	1.99	165.69
VWF	-6.91e-04	11577	GTEx	DepMap	Descartes	0.64	9.07

Kidney Primordium (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. :
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.55e-01
Mean rank of genes in gene set: 8776
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LYPD1	-0.0003344	8776	GTEx	DepMap	Descartes	0.05	2.9

EMT I melanoma (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this EMT program was melanoma specific.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.65e-01
Mean rank of genes in gene set: 7784.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CAV1	0.0026129	252	GTEx	DepMap	Descartes	4.36	363.03
RRAS	-0.0005385	10826	GTEx	DepMap	Descartes	0.31	74.79
TPM1	-0.0010301	12276	GTEx	DepMap	Descartes	1.30	71.95

Schwann cell precursors (Dong)
Obtained from Fig. 3c of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.70e-01
Mean rank of genes in gene set: 8954
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PLP1	-0.0003478	8954	GTEx	DepMap	Descartes	0.52	44

Schwann cell precursors (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. The authors data suggest these SCPs can give rise to symphathoblasts, which can then give rise to chromaffin cells. However, these SCPs can also directly give rise to chromaffin cells (i.e there are two bridges/paths).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.70e-01
Mean rank of genes in gene set: 8954
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PLP1	-0.0003478	8954	GTEx	DepMap	Descartes	0.52	44

M2 Macrophage
These genes were collated from multiple sources:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.00e-01
Mean rank of genes in gene set: 7425.57
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
VEGFA	0.0024755	273	GTEx	DepMap	Descartes	5.77	132.57
IL13	-0.0000208	4152	GTEx	DepMap	Descartes	0.00	0.38
ARG1	-0.0001004	5152	GTEx	DepMap	Descartes	0.00	0.00
CD14	-0.0003068	8430	GTEx	DepMap	Descartes	0.34	53.62
IL10	-0.0005270	10749	GTEx	DepMap	Descartes	0.08	6.62
CD163	-0.0005307	10779	GTEx	DepMap	Descartes	0.05	0.97
TGFB1	-0.0013051	12444	GTEx	DepMap	Descartes	0.13	11.05

Stromal 3 (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Stroma subcluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.16e-01
Mean rank of genes in gene set: 8157.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
OLFML3	0.0034178	137	GTEx	DepMap	Descartes	2.93	350.57
PIK3R1	-0.0009183	12138	GTEx	DepMap	Descartes	0.91	36.08
NOTCH3	-0.0009601	12198	GTEx	DepMap	Descartes	0.87	21.01

Bridge (Kildisiute)
Bridge cell (cites Furlan 2017) markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.28e-01
Mean rank of genes in gene set: 9707
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
DLL3	-0.0004103	9707	GTEx	DepMap	Descartes	0	0.35

Axon guidance (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are for axon guidance instead of myelination:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.39e-01
Mean rank of genes in gene set: 9864
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SEMA3B	-0.0004244	9864	GTEx	DepMap	Descartes	0.3	22.54

Myofibroblastic CAF
These marker genes were curated across cancer subtypes in multiple organ systems as reviewed in Lavie et. al. (PMID 35883004) and contain myofibroblastic specific CAF genes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.40e-01
Mean rank of genes in gene set: 6789.02
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LUM	0.0079736	8	GTEx	DepMap	Descartes	48.81	4979.99
THBS1	0.0067087	16	GTEx	DepMap	Descartes	38.07	1452.17
DCN	0.0066557	18	GTEx	DepMap	Descartes	65.98	2707.49
FN1	0.0064211	22	GTEx	DepMap	Descartes	26.36	784.78
THBS2	0.0044823	68	GTEx	DepMap	Descartes	9.63	402.76
MMP2	0.0027736	224	GTEx	DepMap	Descartes	6.32	451.38
VCAN	0.0027457	228	GTEx	DepMap	Descartes	8.44	194.54
CNN3	0.0026154	250	GTEx	DepMap	Descartes	3.69	516.70
VEGFA	0.0024755	273	GTEx	DepMap	Descartes	5.77	132.57
IGFBP7	0.0024366	285	GTEx	DepMap	Descartes	60.95	10781.25
COL15A1	0.0021463	354	GTEx	DepMap	Descartes	2.84	126.11
COL8A1	0.0013698	666	GTEx	DepMap	Descartes	3.12	114.34
TGFBR2	0.0013672	672	GTEx	DepMap	Descartes	2.85	124.58
PGF	0.0006336	1458	GTEx	DepMap	Descartes	0.48	38.57
COL12A1	0.0003769	2075	GTEx	DepMap	Descartes	2.10	48.96
COL4A1	0.0003358	2188	GTEx	DepMap	Descartes	4.61	161.44
COL5A2	0.0001650	2880	GTEx	DepMap	Descartes	2.22	75.32
IGFBP3	0.0000810	3320	GTEx	DepMap	Descartes	1.25	106.10
TGFBR1	0.0000341	3663	GTEx	DepMap	Descartes	0.63	25.82
COL1A2	0.0000118	3867	GTEx	DepMap	Descartes	31.50	1208.43
COL11A1	-0.0001150	5394	GTEx	DepMap	Descartes	0.24	6.05
TNC	-0.0002288	7290	GTEx	DepMap	Descartes	0.97	29.11
COL13A1	-0.0002896	8176	GTEx	DepMap	Descartes	0.02	0.48
MMP11	-0.0003237	8626	GTEx	DepMap	Descartes	0.11	7.07
COL5A1	-0.0003333	8764	GTEx	DepMap	Descartes	2.94	76.84
TMEM119	-0.0003473	8947	GTEx	DepMap	Descartes	0.59	57.98
COL10A1	-0.0003475	8950	GTEx	DepMap	Descartes	0.24	9.30
ACTG2	-0.0003691	9213	GTEx	DepMap	Descartes	0.03	2.78
WNT5A	-0.0004191	9808	GTEx	DepMap	Descartes	0.04	0.87
TGFB2	-0.0004392	10008	GTEx	DepMap	Descartes	0.20	6.96
TPM2	-0.0004673	10249	GTEx	DepMap	Descartes	1.24	194.86
ITGA7	-0.0004819	10387	GTEx	DepMap	Descartes	0.24	14.51
COL3A1	-0.0005424	10850	GTEx	DepMap	Descartes	30.42	1123.54
POSTN	-0.0005573	10943	GTEx	DepMap	Descartes	1.42	72.90
MYH11	-0.0005703	11031	GTEx	DepMap	Descartes	0.33	8.87
COL1A1	-0.0006378	11381	GTEx	DepMap	Descartes	56.71	2008.04
HOPX	-0.0007689	11801	GTEx	DepMap	Descartes	0.06	3.25
MYL9	-0.0007798	11839	GTEx	DepMap	Descartes	1.77	147.97
MEF2C	-0.0009865	12234	GTEx	DepMap	Descartes	0.12	2.65
TPM1	-0.0010301	12276	GTEx	DepMap	Descartes	1.30	71.95
RGS5	-0.0010478	12303	GTEx	DepMap	Descartes	1.81	37.29
ACTA2	-0.0010597	12313	GTEx	DepMap	Descartes	1.29	188.77
MYLK	-0.0012214	12414	GTEx	DepMap	Descartes	0.57	12.25
TAGLN	-0.0012449	12422	GTEx	DepMap	Descartes	2.70	174.02
TGFB1	-0.0013051	12444	GTEx	DepMap	Descartes	0.13	11.05
CNN2	-0.0014564	12484	GTEx	DepMap	Descartes	0.77	85.31
COL14A1	-0.0017143	12521	GTEx	DepMap	Descartes	2.08	48.33
THY1	-0.0017699	12526	GTEx	DepMap	Descartes	0.54	20.55
BGN	-0.0018968	12533	GTEx	DepMap	Descartes	3.72	283.29

Meta_7 undefined sympathetic (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.55e-01
Mean rank of genes in gene set: 8489
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
TUBB2A	0.0006734	1381	GTEx	DepMap	Descartes	2.14	328.30
DBH	-0.0007819	11843	GTEx	DepMap	Descartes	0.01	0.90
CHGA	-0.0010019	12243	GTEx	DepMap	Descartes	0.20	16.61

Definitive Zone (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Definitive Zone subcluster, which is part of the Cortex cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.61e-01
Mean rank of genes in gene set: 8237.25
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GREB1	0.0002632	2440	GTEx	DepMap	Descartes	0.13	4.33
CALN1	-0.0001699	6302	GTEx	DepMap	Descartes	0.00	0.03
CHCHD10	-0.0007382	11724	GTEx	DepMap	Descartes	0.24	48.12
CPE	-0.0014512	12483	GTEx	DepMap	Descartes	1.10	96.09

Myocytes (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that MYH3+ Myocytes were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.73e-01
Mean rank of genes in gene set: 9191
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MYH3	-0.0001815	6484	GTEx	DepMap	Descartes	0.01	0.58
TTN	-0.0008014	11898	GTEx	DepMap	Descartes	0.03	0.06

B cells (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.82e-01
Mean rank of genes in gene set: 10571
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD79A	-0.0005041	10571	GTEx	DepMap	Descartes	0.01	2.2

Nerves and neurofiliment (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - NF200 is expressed in neurofiliments and is found close to SCPs in normal tissue, NRG1 is secreted by neurons, to which ERBB3 (on glia) binds.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.00e-01
Mean rank of genes in gene set: 10920
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
NRG1	-0.0005533	10920	GTEx	DepMap	Descartes	0.04	0.65

Normal Schwann cell precurors, but not Neuroblastoma Scps (Olsen)
Based on their neuroblastoma data and stated on page 6 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.01e-01
Mean rank of genes in gene set: 10943
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
POSTN	-0.0005573	10943	GTEx	DepMap	Descartes	1.42	72.9

Myelination (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are state as being involved in Myelination and are expressed in the Schwann cell precursor cluster of their UMAP (Fig. 1D), these are suggested to represent more mature myelinating Schwann cells. MPZ is Myelin Protien Zero.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.14e-01
Mean rank of genes in gene set: 9774.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MPZ	-0.0002955	8254	GTEx	DepMap	Descartes	0.07	7.02
LGI4	-0.0006196	11295	GTEx	DepMap	Descartes	0.18	14.33

Chromaffin cells Fig2 (Kamenva)
Marker genes were obtained from Fig. 2H of Kameneva et al (PMID 33833454) for chromaffin cells (differentiating from symphathoblasts):
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.16e-01
Mean rank of genes in gene set: 9162.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PENK	0.0000682	3409	GTEx	DepMap	Descartes	0.27	59.96
CHGB	-0.0007790	11836	GTEx	DepMap	Descartes	0.08	5.47
CHGA	-0.0010019	12243	GTEx	DepMap	Descartes	0.20	16.61

Schwann cell precursor Fig2 (Kamenva)
Marker genes were obtained from Fig. 2H of Kameneva et al (PMID 33833454). These genes were used to show cells transitioning from a SCP to a symphathoblast state in their human fetal single-cell RNA-seq data.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.21e-01
Mean rank of genes in gene set: 7893.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GAS7	0.0034531	133	GTEx	DepMap	Descartes	3.00	98.65
NGFR	0.0007967	1182	GTEx	DepMap	Descartes	2.77	225.90
COL2A1	-0.0001378	5774	GTEx	DepMap	Descartes	0.01	0.63
MPZ	-0.0002955	8254	GTEx	DepMap	Descartes	0.07	7.02
S100B	-0.0003021	8365	GTEx	DepMap	Descartes	1.03	208.30
PLP1	-0.0003478	8954	GTEx	DepMap	Descartes	0.52	44.00
POSTN	-0.0005573	10943	GTEx	DepMap	Descartes	1.42	72.90
ERBB3	-0.0006584	11457	GTEx	DepMap	Descartes	0.04	1.80
MOXD1	-0.0007277	11692	GTEx	DepMap	Descartes	0.13	6.42
MBP	-0.0009463	12181	GTEx	DepMap	Descartes	0.16	4.01

Meta_8 undefined undifferentiated neuronal (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.27e-01
Mean rank of genes in gene set: 9989.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CTNNB1	-0.0002474	7569	GTEx	DepMap	Descartes	1.81	92.92
SOX11	-0.0012139	12410	GTEx	DepMap	Descartes	0.02	0.19

Fetal Zone (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Fetal Zone subcluster, which is part of the Cortex cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.31e-01
Mean rank of genes in gene set: 11645
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GRAMD1B	-0.0007126	11645	GTEx	DepMap	Descartes	0.04	1.04

Stromal 1 (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Stroma subcluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.37e-01
Mean rank of genes in gene set: 8374.43
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
HMGA2	0.0006941	1343	GTEx	DepMap	Descartes	0.08	2.32
MMP14	0.0006808	1363	GTEx	DepMap	Descartes	1.55	104.41
TGFB2	-0.0004392	10008	GTEx	DepMap	Descartes	0.20	6.96
COL27A1	-0.0004775	10340	GTEx	DepMap	Descartes	0.26	8.02
IGSF3	-0.0006729	11516	GTEx	DepMap	Descartes	0.02	0.48
CHD3	-0.0006784	11543	GTEx	DepMap	Descartes	0.86	31.42
NFIB	-0.0015600	12508	GTEx	DepMap	Descartes	1.82	53.81

Chromaffin cell-like (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.41e-01
Mean rank of genes in gene set: 10283.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
NTRK1	-0.0003912	9506	GTEx	DepMap	Descartes	0.00	0.21
NPY	-0.0005754	11061	GTEx	DepMap	Descartes	0.02	4.26

Schwann cell precursor (Kildisiute)
SCP markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.42e-01
Mean rank of genes in gene set: 9555
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MPZ	-0.0002955	8254	GTEx	DepMap	Descartes	0.07	7.02
PLP1	-0.0003478	8954	GTEx	DepMap	Descartes	0.52	44.00
ERBB3	-0.0006584	11457	GTEx	DepMap	Descartes	0.04	1.80

Schwann cell precursors (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.42e-01
Mean rank of genes in gene set: 9555
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MPZ	-0.0002955	8254	GTEx	DepMap	Descartes	0.07	7.02
PLP1	-0.0003478	8954	GTEx	DepMap	Descartes	0.52	44.00
ERBB3	-0.0006584	11457	GTEx	DepMap	Descartes	0.04	1.80

Committed Progenitor (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Committed Progenitor subcluster (seems to mean progenitor of chromaffin cells), which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.43e-01
Mean rank of genes in gene set: 7748.93
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ATP6V1B2	0.0029639	196	GTEx	DepMap	Descartes	3.97	153.53
MEG3	0.0017185	501	GTEx	DepMap	Descartes	9.94	209.52
UNC5C	0.0004029	1997	GTEx	DepMap	Descartes	0.11	2.76
NRK	0.0003174	2250	GTEx	DepMap	Descartes	0.18	5.57
ZDBF2	0.0001729	2843	GTEx	DepMap	Descartes	0.41	10.68
SYTL4	-0.0002940	8234	GTEx	DepMap	Descartes	0.19	10.98
SYT1	-0.0004036	9649	GTEx	DepMap	Descartes	0.15	10.71
DGKK	-0.0004533	10137	GTEx	DepMap	Descartes	0.00	0.03
NTRK3	-0.0004701	10272	GTEx	DepMap	Descartes	0.27	4.16
SLC18A2	-0.0005668	11012	GTEx	DepMap	Descartes	0.00	0.30
NDUFA4L2	-0.0006750	11528	GTEx	DepMap	Descartes	0.06	6.22
FAM155A	-0.0007249	11685	GTEx	DepMap	Descartes	0.04	0.78
RGS4	-0.0007613	11781	GTEx	DepMap	Descartes	0.37	17.67
PARM1	-0.0007828	11846	GTEx	DepMap	Descartes	0.06	2.55
RGS5	-0.0010478	12303	GTEx	DepMap	Descartes	1.81	37.29

Schwann cell precursor (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are stated as being characteristic of Schwann cell lineage and Furlan et al. (Science 2017, PMID 28684471) is referenced to support this. These cell types seem to be claimed to form a bridge between the mesenchymal and adrenergic cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.44e-01
Mean rank of genes in gene set: 9592
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
S100B	-0.0003021	8365	GTEx	DepMap	Descartes	1.03	208.3
PLP1	-0.0003478	8954	GTEx	DepMap	Descartes	0.52	44.0
ERBB3	-0.0006584	11457	GTEx	DepMap	Descartes	0.04	1.8

Schwann cell precursor-like Fig 1D (Olsen)
Similar to above, but for selected SCP marker genes.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.44e-01
Mean rank of genes in gene set: 9592
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
S100B	-0.0003021	8365	GTEx	DepMap	Descartes	1.03	208.3
PLP1	-0.0003478	8954	GTEx	DepMap	Descartes	0.52	44.0
ERBB3	-0.0006584	11457	GTEx	DepMap	Descartes	0.04	1.8

PMN-MDSC
These marker genes were curated for MDSC subtypes as reviewed in Veglia et. al. (PMID 33526920):
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.46e-01
Mean rank of genes in gene set: 7726
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ANXA1	0.0049648	49	GTEx	DepMap	Descartes	19.94	2433.59
PTGS2	0.0019586	407	GTEx	DepMap	Descartes	5.17	290.01
TNFRSF10B	0.0011407	830	GTEx	DepMap	Descartes	1.49	94.95
STAT3	0.0009139	1046	GTEx	DepMap	Descartes	5.53	298.26
ARG1	-0.0001004	5152	GTEx	DepMap	Descartes	0.00	0.00
SLC27A2	-0.0002368	7425	GTEx	DepMap	Descartes	0.00	0.24
IRF1	-0.0002746	7956	GTEx	DepMap	Descartes	3.54	216.64
CSF1	-0.0002849	8110	GTEx	DepMap	Descartes	0.98	59.10
ARG2	-0.0004600	10192	GTEx	DepMap	Descartes	0.14	19.60
CD36	-0.0005064	10587	GTEx	DepMap	Descartes	0.15	2.42
STAT1	-0.0007269	11689	GTEx	DepMap	Descartes	0.84	50.91
IL1B	-0.0007689	11800	GTEx	DepMap	Descartes	1.82	165.57
HIF1A	-0.0008201	11940	GTEx	DepMap	Descartes	1.58	88.99
STAT6	-0.0008329	11969	GTEx	DepMap	Descartes	0.70	43.65
CD84	-0.0008571	12020	GTEx	DepMap	Descartes	0.02	0.61
TGFB1	-0.0013051	12444	GTEx	DepMap	Descartes	0.13	11.05

Adrenergic neuroblatoma (Olsen)
Stated on page 6 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - neuroblastoma adrenergic markers derived from their data, which are state as resembling sympathoblasts and lacking chromffin markers such as PNMT:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.49e-01
Mean rank of genes in gene set: 12194
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GAP43	-0.0009569	12194	GTEx	DepMap	Descartes	0.08	9.33

Cytotoxic T cells (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.49e-01
Mean rank of genes in gene set: 12202
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD8A	-0.0009653	12202	GTEx	DepMap	Descartes	0.01	1.15

Symphathoadrenal cells (Yuan)
Obtained from Fig. 1 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.50e-01
Mean rank of genes in gene set: 12243
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CHGA	-0.0010019	12243	GTEx	DepMap	Descartes	0.2	16.61

Bridge region SCP-like (Olsen)
As above but for the SCP-like region:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.53e-01
Mean rank of genes in gene set: 9312.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MPZ	-0.0002955	8254	GTEx	DepMap	Descartes	0.07	7.02
S100B	-0.0003021	8365	GTEx	DepMap	Descartes	1.03	208.30
FXYD1	-0.0003659	9175	GTEx	DepMap	Descartes	0.47	126.89
ERBB3	-0.0006584	11457	GTEx	DepMap	Descartes	0.04	1.80

Adrenergic immature (Olsen)
Stated on pages 6 and 7 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - it is argued that upon reanalysis of the adrenergic neuroblastoma cells, there is further heterogeneity, grouped into proliferating, mature and immature cell populations. These is a marker listed for the immature adrenergic cells. SOX11 is referenced as important in early phases of pro-adrenergic differentiation (cites PMID 20147379) whereas SOX4 appears later. SOX11 is also associated with poor outcome in neuroblatoma bulk RNA-seq data (Olsen analysis).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.55e-01
Mean rank of genes in gene set: 12410
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SOX11	-0.0012139	12410	GTEx	DepMap	Descartes	0.02	0.19

Leukocyte (Kildisiute)
Leukocyes markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.58e-01
Mean rank of genes in gene set: 12523
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PTPRC	-0.0017417	12523	GTEx	DepMap	Descartes	0.09	4.82

Adrenergic mature (olsen)
Stated on pages 6 and 7 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - it is argued that upon reanalysis of the adrenergic neuroblastoma cells, there is further heterogeneity, grouped into proliferating, mature and immature cell populations. These markers are specific to the mature adrenergic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.63e-01
Mean rank of genes in gene set: 10850
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
NTRK1	-0.0003912	9506	GTEx	DepMap	Descartes	0.00	0.21
GAP43	-0.0009569	12194	GTEx	DepMap	Descartes	0.08	9.33

Meta_9 ATRX (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.65e-01
Mean rank of genes in gene set: 10925.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ATRX	-0.0004647	10233	GTEx	DepMap	Descartes	2.21	51.13
ZFHX3	-0.0007045	11618	GTEx	DepMap	Descartes	1.20	18.67

SCPs mouse and neuroblastoma (Olsen)
Similar to above, but for genes common to neuroblastoma SCP-like cells (as determined from their UMAP plot) and mouse SCP cells.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.71e-01
Mean rank of genes in gene set: 8338.91
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CNN3	0.0026154	250	GTEx	DepMap	Descartes	3.69	516.70
DST	0.0020120	393	GTEx	DepMap	Descartes	4.72	57.94
MPZ	-0.0002955	8254	GTEx	DepMap	Descartes	0.07	7.02
S100B	-0.0003021	8365	GTEx	DepMap	Descartes	1.03	208.30
PLP1	-0.0003478	8954	GTEx	DepMap	Descartes	0.52	44.00
FXYD1	-0.0003659	9175	GTEx	DepMap	Descartes	0.47	126.89
SEMA3B	-0.0004244	9864	GTEx	DepMap	Descartes	0.30	22.54
LGI4	-0.0006196	11295	GTEx	DepMap	Descartes	0.18	14.33
ERBB3	-0.0006584	11457	GTEx	DepMap	Descartes	0.04	1.80
GPM6B	-0.0007198	11669	GTEx	DepMap	Descartes	0.23	11.81
NRXN1	-0.0008686	12052	GTEx	DepMap	Descartes	0.10	2.29

M-MDSC
These marker genes were curated for MDSC subtypes as reviewed in Veglia et. al. (PMID 33526920):
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.72e-01
Mean rank of genes in gene set: 8067.47
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
VEGFA	0.0024755	273	GTEx	DepMap	Descartes	5.77	132.57
TNFRSF10B	0.0011407	830	GTEx	DepMap	Descartes	1.49	94.95
STAT3	0.0009139	1046	GTEx	DepMap	Descartes	5.53	298.26
ARG1	-0.0001004	5152	GTEx	DepMap	Descartes	0.00	0.00
NOS2	-0.0001257	5555	GTEx	DepMap	Descartes	0.00	0.05
CD14	-0.0003068	8430	GTEx	DepMap	Descartes	0.34	53.62
CD274	-0.0003450	8906	GTEx	DepMap	Descartes	0.06	3.01
ARG2	-0.0004600	10192	GTEx	DepMap	Descartes	0.14	19.60
CD36	-0.0005064	10587	GTEx	DepMap	Descartes	0.15	2.42
IL10	-0.0005270	10749	GTEx	DepMap	Descartes	0.08	6.62
TNF	-0.0005801	11088	GTEx	DepMap	Descartes	0.04	3.99
IL1B	-0.0007689	11800	GTEx	DepMap	Descartes	1.82	165.57
HIF1A	-0.0008201	11940	GTEx	DepMap	Descartes	1.58	88.99
CD84	-0.0008571	12020	GTEx	DepMap	Descartes	0.02	0.61
TGFB1	-0.0013051	12444	GTEx	DepMap	Descartes	0.13	11.05

Adrenergic proliferating cluster (Olsen)
Stated on pages 6 and 7 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - it is argued that upon reanalysis of the adrenergic neuroblastoma cells, there is further heterogeneity, grouped into proliferating, mature and immature cell populations. These genes were found as markers for proliferating adrenergic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.77e-01
Mean rank of genes in gene set: 11390.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13
EZH2	-0.0007932	11872	GTEx	DepMap	Descartes	0.33	23.17

Noninflammatory Macrophages (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.79e-01
Mean rank of genes in gene set: 11509
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD163	-0.0005307	10779	GTEx	DepMap	Descartes	0.05	0.97
MAF	-0.0009928	12239	GTEx	DepMap	Descartes	0.17	6.30

Neuroblastoma (Kildisiute)
Neuroblastoma markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.82e-01
Mean rank of genes in gene set: 11641
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MYCN	-0.0005853	11112	GTEx	DepMap	Descartes	0.06	4.44
PHOX2A	-0.0009391	12170	GTEx	DepMap	Descartes	0.00	0.00

EMT III (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this EMT program was enriched in non-cycling cells.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.82e-01
Mean rank of genes in gene set: 10080
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LAMB3	-0.0002841	8102	GTEx	DepMap	Descartes	0.01	0.37
ITGA2	-0.0004195	9820	GTEx	DepMap	Descartes	0.16	3.80
LAMA3	-0.0004752	10316	GTEx	DepMap	Descartes	0.02	0.62
JUP	-0.0008836	12082	GTEx	DepMap	Descartes	0.13	6.72

Adrenergic (Olsen)
Markers listed in on page 3 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are for adrenergic genes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.82e-01
Mean rank of genes in gene set: 11667
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
TH	-0.0006678	11491	GTEx	DepMap	Descartes	0.02	2.2
DBH	-0.0007819	11843	GTEx	DepMap	Descartes	0.01	0.9

Chromaffin (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. These markers are expressed at a lower level in symphathoblasts.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.85e-01
Mean rank of genes in gene set: 11867
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
TH	-0.0006678	11491	GTEx	DepMap	Descartes	0.02	2.20
CHGA	-0.0010019	12243	GTEx	DepMap	Descartes	0.20	16.61

HSCs and immune cells (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.87e-01
Mean rank of genes in gene set: 10956.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
RGS10	-0.0004942	10496	GTEx	DepMap	Descartes	0.35	106.46
CD163	-0.0005307	10779	GTEx	DepMap	Descartes	0.05	0.97
AIF1	-0.0006986	11595	GTEx	DepMap	Descartes	0.04	5.22

Vascular (Kildisiute)
Vascular markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.88e-01
Mean rank of genes in gene set: 11009.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
KDR	-0.0003831	9404	GTEx	DepMap	Descartes	0.12	3.02
PTPRB	-0.0007476	11750	GTEx	DepMap	Descartes	0.13	1.26
PLVAP	-0.0007936	11875	GTEx	DepMap	Descartes	0.25	14.38

Translation (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.88e-01
Mean rank of genes in gene set: 11018
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
EIF3E	-0.0003987	9588	GTEx	DepMap	Descartes	1.74	225.72
EIF3F	-0.0006322	11361	GTEx	DepMap	Descartes	1.06	37.99
EIF3L	-0.0008941	12105	GTEx	DepMap	Descartes	1.68	132.45

Chromaffin Cells (Dong)
Obtained from Fig. 3c of Dong et al. (PMID 32946775). Jansky et al (PMID) criticize this choice of markers, stating: the neuronal markers NPY, PRPH, NTRK1 and ISL1 are used to annotate chromaffin cells; these were clearly expressed in neuroblasts in our analyses.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.91e-01
Mean rank of genes in gene set: 11228
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
NTRK1	-0.0003912	9506	GTEx	DepMap	Descartes	0.00	0.21
ISL1	-0.0008517	12008	GTEx	DepMap	Descartes	0.02	1.05
PHOX2A	-0.0009391	12170	GTEx	DepMap	Descartes	0.00	0.00

Immune cells (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that PTPRC+ Immune cells were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.92e-01
Mean rank of genes in gene set: 12392.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD247	-0.0010202	12262	GTEx	DepMap	Descartes	0.00	0.00
PTPRC	-0.0017417	12523	GTEx	DepMap	Descartes	0.09	4.82

Meta5 unclear (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.92e-01
Mean rank of genes in gene set: 12415.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
STMN2	-0.0010938	12342	GTEx	DepMap	Descartes	0.04	4.35
STMN1	-0.0014764	12489	GTEx	DepMap	Descartes	0.61	52.19

Chromaffin cell development (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.92e-01
Mean rank of genes in gene set: 12423.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
HAND2	-0.0010967	12347	GTEx	DepMap	Descartes	0.03	1.42
GATA3	-0.0015147	12500	GTEx	DepMap	Descartes	0.02	0.79

Cell cycle (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.92e-01
Mean rank of genes in gene set: 11311.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13
TOP2A	-0.0006705	11507	GTEx	DepMap	Descartes	0.03	1.27
PCNA	-0.0006731	11519	GTEx	DepMap	Descartes	0.30	62.13

Immune (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the broad Immune cluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.92e-01
Mean rank of genes in gene set: 12485
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LCP2	-0.0012543	12425	GTEx	DepMap	Descartes	0.11	4.27
TMSB4X	-0.0021726	12545	GTEx	DepMap	Descartes	11.15	1540.93

Immune cells (Yuan)
Obtained from Fig. 1 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.93e-01
Mean rank of genes in gene set: 12525.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PTPRC	-0.0017417	12523	GTEx	DepMap	Descartes	0.09	4.82
CXCR4	-0.0017844	12528	GTEx	DepMap	Descartes	0.19	24.14

Endothelium (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.93e-01
Mean rank of genes in gene set: 10701.25
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
KDR	-0.0003831	9404	GTEx	DepMap	Descartes	0.12	3.02
PRCP	-0.0005441	10864	GTEx	DepMap	Descartes	0.54	17.00
EGFL7	-0.0005538	10927	GTEx	DepMap	Descartes	0.05	4.21
FLT1	-0.0007029	11610	GTEx	DepMap	Descartes	0.22	4.56

Endothelial cells (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that PTPRB+ Endothelial cells were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.93e-01
Mean rank of genes in gene set: 11429
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
EGFL7	-0.0005538	10927	GTEx	DepMap	Descartes	0.05	4.21
FLT1	-0.0007029	11610	GTEx	DepMap	Descartes	0.22	4.56
PTPRB	-0.0007476	11750	GTEx	DepMap	Descartes	0.13	1.26

M1 Macrophage
These genes were collated from multiple sources:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.93e-01
Mean rank of genes in gene set: 8980.27
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CCL2	0.0080405	7	GTEx	DepMap	Descartes	69.43	21560.64
TLR4	-0.0000513	4450	GTEx	DepMap	Descartes	0.15	2.49
CD80	-0.0002846	8109	GTEx	DepMap	Descartes	0.01	0.40
CD14	-0.0003068	8430	GTEx	DepMap	Descartes	0.34	53.62
CXCL16	-0.0004100	9705	GTEx	DepMap	Descartes	0.33	35.83
IL18	-0.0004104	9710	GTEx	DepMap	Descartes	0.06	8.09
TNF	-0.0005801	11088	GTEx	DepMap	Descartes	0.04	3.99
IL33	-0.0006483	11425	GTEx	DepMap	Descartes	0.36	31.43
ITGAX	-0.0007120	11640	GTEx	DepMap	Descartes	0.04	1.44
IL1B	-0.0007689	11800	GTEx	DepMap	Descartes	1.82	165.57
CCL5	-0.0012415	12419	GTEx	DepMap	Descartes	0.07	8.86

Sympathobasts or chromaffin (Kildisiute)
Sympathoblast and chromaffin markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.95e-01
Mean rank of genes in gene set: 11723.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
TH	-0.0006678	11491	GTEx	DepMap	Descartes	0.02	2.20
CHGB	-0.0007790	11836	GTEx	DepMap	Descartes	0.08	5.47
DBH	-0.0007819	11843	GTEx	DepMap	Descartes	0.01	0.90

Proliferating Cells
These are canonical marker genes of cycling cells, obtained from Fig. 3 of Hsiao et al. (PMID 32312741):
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.96e-01
Mean rank of genes in gene set: 11048.25
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
UBE2C	-0.0005236	10719	GTEx	DepMap	Descartes	0.00	0.63
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13
CDK1	-0.0005752	11058	GTEx	DepMap	Descartes	0.02	1.42
TOP2A	-0.0006705	11507	GTEx	DepMap	Descartes	0.03	1.27

Sympathoblasts (Kildisiute)
Sympathoblasts markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.96e-01
Mean rank of genes in gene set: 11833.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
NPY	-0.0005754	11061	GTEx	DepMap	Descartes	0.02	4.26
GAP43	-0.0009569	12194	GTEx	DepMap	Descartes	0.08	9.33
BCL2	-0.0010041	12245	GTEx	DepMap	Descartes	0.26	9.24

Myofibroblasts (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that ACTA2+ Myofibroblasts were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.97e-01
Mean rank of genes in gene set: 11922
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MYH11	-0.0005703	11031	GTEx	DepMap	Descartes	0.33	8.87
ACTA2	-0.0010597	12313	GTEx	DepMap	Descartes	1.29	188.77
TAGLN	-0.0012449	12422	GTEx	DepMap	Descartes	2.70	174.02

Stress response (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.97e-01
Mean rank of genes in gene set: 12039.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
JUN	-0.0005701	11028	GTEx	DepMap	Descartes	9.03	790.91
FOS	-0.0020392	12541	GTEx	DepMap	Descartes	14.24	2035.05
JUNB	-0.0026445	12549	GTEx	DepMap	Descartes	10.12	1496.34

Bridge region adrenergic transition (Olsen)
As above but for cells in the mesenchymal transitioning to adrenergic region:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-01
Mean rank of genes in gene set: 12259.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MLLT11	-0.0009101	12133	GTEx	DepMap	Descartes	0.32	33.45
RGS5	-0.0010478	12303	GTEx	DepMap	Descartes	1.81	37.29
STMN2	-0.0010938	12342	GTEx	DepMap	Descartes	0.04	4.35

Bridge region mesenchymal-SCP transition (Olsen)
As above but for cells in the mesenchymal transitioning to SCP region:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-01
Mean rank of genes in gene set: 12372.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IFITM3	-0.0008710	12060	GTEx	DepMap	Descartes	14.06	4764.09
S100A10	-0.0016193	12510	GTEx	DepMap	Descartes	1.08	354.34
B2M	-0.0024801	12548	GTEx	DepMap	Descartes	22.34	2254.76

Bridge region SCP-adrenergic transition (Olsen)
As above but for cells in the mesenchymal transitioning to SCP-adrenergic region:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-01
Mean rank of genes in gene set: 11595
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PCBP2	-0.0005797	11083	GTEx	DepMap	Descartes	3.15	259.29
MDK	-0.0007123	11641	GTEx	DepMap	Descartes	1.75	306.04
SRP14	-0.0007392	11726	GTEx	DepMap	Descartes	2.18	493.39
HMGB1	-0.0008170	11930	GTEx	DepMap	Descartes	2.17	105.13

Late Neuroblasts (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types. Note: the Jansky Late Neuroblast signature is the same as the earlier Neuroblast signature, but NEFM and ALK are now absent (meaning lower expressed at later stages of differentiation):
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.99e-01
Mean rank of genes in gene set: 11098.8
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SYN3	-0.0002806	8047	GTEx	DepMap	Descartes	0.00	0.00
IL7	-0.0005502	10903	GTEx	DepMap	Descartes	0.01	0.62
ISL1	-0.0008517	12008	GTEx	DepMap	Descartes	0.02	1.05
GAP43	-0.0009569	12194	GTEx	DepMap	Descartes	0.08	9.33
STMN2	-0.0010938	12342	GTEx	DepMap	Descartes	0.04	4.35

Chromaffin and connecting progenitor cells (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.99e-01
Mean rank of genes in gene set: 11711.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
DDC	-0.0006161	11270	GTEx	DepMap	Descartes	0.00	0.00
TH	-0.0006678	11491	GTEx	DepMap	Descartes	0.02	2.20
DBH	-0.0007819	11843	GTEx	DepMap	Descartes	0.01	0.90
CHGA	-0.0010019	12243	GTEx	DepMap	Descartes	0.20	16.61

Late chromaffin cells (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types. Note: this late chromaffin cell signature also includes PNMT, which is an enzyme catalyzing methylation of norepinephrine to form epinephrine; this marker is absent from the ealier less differentiated chromaffin cells (which may have been referred to as sympathoblasts in other datasets, I think):
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.99e-01
Mean rank of genes in gene set: 11711.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
DDC	-0.0006161	11270	GTEx	DepMap	Descartes	0.00	0.00
TH	-0.0006678	11491	GTEx	DepMap	Descartes	0.02	2.20
DBH	-0.0007819	11843	GTEx	DepMap	Descartes	0.01	0.90
CHGA	-0.0010019	12243	GTEx	DepMap	Descartes	0.20	16.61

Bridge (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Bridge subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.99e-01
Mean rank of genes in gene set: 9428.58
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
RCC2	0.0001943	2738	GTEx	DepMap	Descartes	0.82	50.84
WDR6	-0.0000871	4936	GTEx	DepMap	Descartes	0.70	45.32
CDKN1C	-0.0001085	5281	GTEx	DepMap	Descartes	1.56	214.35
TBX20	-0.0001922	6665	GTEx	DepMap	Descartes	0.00	0.00
DLL3	-0.0004103	9707	GTEx	DepMap	Descartes	0.00	0.35
NFASC	-0.0005006	10549	GTEx	DepMap	Descartes	0.17	3.49
GSE1	-0.0006736	11521	GTEx	DepMap	Descartes	0.34	NA
MIAT	-0.0010295	12275	GTEx	DepMap	Descartes	0.02	0.46
LDHB	-0.0010440	12298	GTEx	DepMap	Descartes	0.97	157.06
DPYSL3	-0.0010526	12309	GTEx	DepMap	Descartes	0.74	26.66
SOX11	-0.0012139	12410	GTEx	DepMap	Descartes	0.02	0.19
CKB	-0.0013440	12454	GTEx	DepMap	Descartes	0.31	63.66

Antigen Presenting CAF
These marker genes were curated across cancer subtypes in multiple organ systems as reviewed in Lavie et. al. (PMID 35883004) and contain antigen presenting specific CAF genes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.99e-01
Mean rank of genes in gene set: 9446.25
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
S100A13	0.0005739	1565	GTEx	DepMap	Descartes	0.64	77.33
UPK3B	-0.0000366	4299	GTEx	DepMap	Descartes	0.00	0.08
S100A3	-0.0000913	5006	GTEx	DepMap	Descartes	0.00	1.48
SLPI	-0.0003117	8489	GTEx	DepMap	Descartes	0.00	0.00
KRT8	-0.0003772	9324	GTEx	DepMap	Descartes	0.03	2.34
KRT18	-0.0005129	10643	GTEx	DepMap	Descartes	0.12	6.77
KRT19	-0.0008538	12011	GTEx	DepMap	Descartes	0.01	0.25
S100A4	-0.0009428	12175	GTEx	DepMap	Descartes	0.59	231.32
CD83	-0.0011833	12399	GTEx	DepMap	Descartes	0.22	19.72
CCL5	-0.0012415	12419	GTEx	DepMap	Descartes	0.07	8.86
S100A10	-0.0016193	12510	GTEx	DepMap	Descartes	1.08	354.34
CD74	-0.0016569	12515	GTEx	DepMap	Descartes	3.79	211.59

Symphathoblasts (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. Several of these markers are also (more weakly) expressed in chromaffin cells. The authors data suggest Symphthoblasts give rise to chromaffin cells.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 12274
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ISL1	-0.0008517	12008	GTEx	DepMap	Descartes	0.02	1.05
STMN2	-0.0010938	12342	GTEx	DepMap	Descartes	0.04	4.35
ELAVL4	-0.0011031	12355	GTEx	DepMap	Descartes	0.01	0.88
ELAVL3	-0.0011724	12391	GTEx	DepMap	Descartes	0.09	4.86

Neuroblasts (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 10901.86
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SYN3	-0.0002806	8047	GTEx	DepMap	Descartes	0.00	0.00
ALK	-0.0003765	9317	GTEx	DepMap	Descartes	0.00	0.13
IL7	-0.0005502	10903	GTEx	DepMap	Descartes	0.01	0.62
NEFM	-0.0006701	11502	GTEx	DepMap	Descartes	0.04	5.26
ISL1	-0.0008517	12008	GTEx	DepMap	Descartes	0.02	1.05
GAP43	-0.0009569	12194	GTEx	DepMap	Descartes	0.08	9.33
STMN2	-0.0010938	12342	GTEx	DepMap	Descartes	0.04	4.35

E Chromaffin (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Epinepherine Chromaffin subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 11314.17
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
DLK1	-0.0004046	9660	GTEx	DepMap	Descartes	0.03	1.85
NPY	-0.0005754	11061	GTEx	DepMap	Descartes	0.02	4.26
RAB3C	-0.0006423	11403	GTEx	DepMap	Descartes	0.01	0.12
RAB3B	-0.0006781	11542	GTEx	DepMap	Descartes	0.01	0.25
ANK3	-0.0008688	12053	GTEx	DepMap	Descartes	0.14	1.66
GATA2	-0.0009356	12166	GTEx	DepMap	Descartes	0.08	4.17

Other T cells (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 11398
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FOXP3	-0.0003128	8501	GTEx	DepMap	Descartes	0.01	1.74
LEF1	-0.0005289	10761	GTEx	DepMap	Descartes	0.37	25.29
CCR7	-0.0009077	12126	GTEx	DepMap	Descartes	0.04	5.27
SELL	-0.0010171	12258	GTEx	DepMap	Descartes	0.14	18.18
CD4	-0.0010948	12344	GTEx	DepMap	Descartes	0.07	6.46
TCF7	-0.0011833	12398	GTEx	DepMap	Descartes	0.09	4.55

Symphathoblasts Fig2 (Kamenva)
Marker genes were obtained from Fig. 2H of Kameneva et al (PMID 33833454) for symphathoblasts (differentiating from SCPs):
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 12128.8
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ELAVL2	-0.0006977	11592	GTEx	DepMap	Descartes	0.01	0.35
ISL1	-0.0008517	12008	GTEx	DepMap	Descartes	0.02	1.05
STMN2	-0.0010938	12342	GTEx	DepMap	Descartes	0.04	4.35
HAND2	-0.0010967	12347	GTEx	DepMap	Descartes	0.03	1.42
ELAVL4	-0.0011031	12355	GTEx	DepMap	Descartes	0.01	0.88

Broad sympathetic marker (Dong)
Obtained from Fig. 3c of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 12030.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
TH	-0.0006678	11491	GTEx	DepMap	Descartes	0.02	2.20
CHGB	-0.0007790	11836	GTEx	DepMap	Descartes	0.08	5.47
DBH	-0.0007819	11843	GTEx	DepMap	Descartes	0.01	0.90
GATA2	-0.0009356	12166	GTEx	DepMap	Descartes	0.08	4.17
HAND2	-0.0010967	12347	GTEx	DepMap	Descartes	0.03	1.42
GATA3	-0.0015147	12500	GTEx	DepMap	Descartes	0.02	0.79

Adrenergic Fig 1D (Olsen)
Similar to above, but for selected adrenergic marker genes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 10899.09
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MAP1B	0.0045458	64	GTEx	DepMap	Descartes	14.86	337.30
NRG1	-0.0005533	10920	GTEx	DepMap	Descartes	0.04	0.65
TH	-0.0006678	11491	GTEx	DepMap	Descartes	0.02	2.20
DBH	-0.0007819	11843	GTEx	DepMap	Descartes	0.01	0.90
ISL1	-0.0008517	12008	GTEx	DepMap	Descartes	0.02	1.05
MLLT11	-0.0009101	12133	GTEx	DepMap	Descartes	0.32	33.45
UCHL1	-0.0009234	12147	GTEx	DepMap	Descartes	0.55	89.07
RTN1	-0.0010348	12284	GTEx	DepMap	Descartes	0.11	10.42
RGS5	-0.0010478	12303	GTEx	DepMap	Descartes	1.81	37.29
STMN2	-0.0010938	12342	GTEx	DepMap	Descartes	0.04	4.35
ELAVL4	-0.0011031	12355	GTEx	DepMap	Descartes	0.01	0.88

N Chromafin (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Norepinepherine Chromaffin subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 10224.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SCG5	-0.0001163	5415	GTEx	DepMap	Descartes	0.26	40.18
ADCYAP1R1	-0.0001324	5682	GTEx	DepMap	Descartes	0.04	1.93
PPFIA2	-0.0002376	7432	GTEx	DepMap	Descartes	0.05	2.49
LGR5	-0.0002854	8117	GTEx	DepMap	Descartes	0.00	0.00
SYN2	-0.0003255	8656	GTEx	DepMap	Descartes	0.01	0.73
CACNA2D1	-0.0004023	9630	GTEx	DepMap	Descartes	0.17	5.67
NAP1L5	-0.0004430	10039	GTEx	DepMap	Descartes	0.11	14.53
PTPRN	-0.0007057	11623	GTEx	DepMap	Descartes	0.01	0.24
PCLO	-0.0007660	11792	GTEx	DepMap	Descartes	0.01	0.10
SLCO3A1	-0.0008063	11910	GTEx	DepMap	Descartes	0.31	15.24
SCG3	-0.0008515	12007	GTEx	DepMap	Descartes	0.01	0.27
SNAP25	-0.0008665	12042	GTEx	DepMap	Descartes	0.01	0.32
CELF4	-0.0008682	12050	GTEx	DepMap	Descartes	0.04	3.32
C1QL1	-0.0009055	12120	GTEx	DepMap	Descartes	0.01	1.60
CXCL14	-0.0019523	12535	GTEx	DepMap	Descartes	0.56	69.75
GNAS	-0.0022169	12546	GTEx	DepMap	Descartes	11.82	713.14

Medulla (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for genes defining the broad group labelled adrenal medulla, which included 7 subclusters (SCP, Bridge, Committed Progenitor, N Chromaffin, E Chromaffin, Neuroblast) - see UMAP on their Fig1B for cluster assignments.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 10903.62
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MAP1B	0.0045458	64	GTEx	DepMap	Descartes	14.86	337.30
CYB561	-0.0004226	9846	GTEx	DepMap	Descartes	0.22	17.86
DISP2	-0.0004477	10087	GTEx	DepMap	Descartes	0.02	0.46
SLC18A1	-0.0004488	10100	GTEx	DepMap	Descartes	0.00	0.00
DDC	-0.0006161	11270	GTEx	DepMap	Descartes	0.00	0.00
TH	-0.0006678	11491	GTEx	DepMap	Descartes	0.02	2.20
CHGB	-0.0007790	11836	GTEx	DepMap	Descartes	0.08	5.47
DBH	-0.0007819	11843	GTEx	DepMap	Descartes	0.01	0.90
EML5	-0.0007909	11868	GTEx	DepMap	Descartes	0.03	0.78
UCHL1	-0.0009234	12147	GTEx	DepMap	Descartes	0.55	89.07
PHOX2A	-0.0009391	12170	GTEx	DepMap	Descartes	0.00	0.00
CHGA	-0.0010019	12243	GTEx	DepMap	Descartes	0.20	16.61
NNAT	-0.0010261	12270	GTEx	DepMap	Descartes	0.01	1.50
HAND2	-0.0010967	12347	GTEx	DepMap	Descartes	0.03	1.42
PCSK1N	-0.0011327	12376	GTEx	DepMap	Descartes	0.07	12.18
GATA3	-0.0015147	12500	GTEx	DepMap	Descartes	0.02	0.79

Neuroblast (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Neuroblast subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 12157.73
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
NEFL	-0.0006100	11243	GTEx	DepMap	Descartes	0.01	0.88
NEFM	-0.0006701	11502	GTEx	DepMap	Descartes	0.04	5.26
ISL1	-0.0008517	12008	GTEx	DepMap	Descartes	0.02	1.05
CCND1	-0.0009977	12240	GTEx	DepMap	Descartes	0.51	24.11
RTN1	-0.0010348	12284	GTEx	DepMap	Descartes	0.11	10.42
STMN2	-0.0010938	12342	GTEx	DepMap	Descartes	0.04	4.35
ELAVL4	-0.0011031	12355	GTEx	DepMap	Descartes	0.01	0.88
ELAVL3	-0.0011724	12391	GTEx	DepMap	Descartes	0.09	4.86
BASP1	-0.0012748	12435	GTEx	DepMap	Descartes	0.95	103.78
INA	-0.0013115	12446	GTEx	DepMap	Descartes	0.04	2.14
STMN1	-0.0014764	12489	GTEx	DepMap	Descartes	0.61	52.19

Plasmacytoid dendritic cells (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Adrenal Cortex (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Symphatoblasts (Dong)
Obtained from Fig. 3c of Dong et al. (PMID 32946775). Jansky et al (PMID) criticize this choice of markers, stating: Sympathoblasts were identified by Dong et al.28 based on the expression of CARTPT and INSM1, which we found expressed in chromaffin cells:
Autonomic neurons mouse (olsen)
Stated on page 6 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - seems to be referencing previous mouse lineage tracing studies in mouse which sucggested SCPs give rise to autonomic neurons and mesenchymal cells:
Erythroblast (Kildisiute)
Erythroblast markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Chromaffin (Kildisiute)
Chromaffin markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Cortex (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for their broad Cortex group, which is composed of Fetal Zone, Definitive Zone and Adr Primordium subclusters:
Adrenal cortical (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that CYP11A1+ Adrenal cortical cells were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:

Below shows ranks on this GEP for literature curated gene lists for large gene sets
These include those reported as mesenchymal/adrenergic by Van Groningen et al.
High ranks indicate this gene is a driver of this GEP (note these results are not ordered).
The Mean Count column shows the mean read count in cells scoring highly (H > 50) on this gene expression program.

VanGroningen Adrenergic Genes
Adrenergic marker genes from Supplementary Table 2 of Van Groningen et al. Nature Genetics 2017. These genes were identified by differential expression analysis of mesenchymal-like and adrenergic-like neuroblastoma cell lines.
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 9065.18
Median rank of genes in gene set: 10708
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
TIAM1	0.0048898	52	GTEx	DepMap	Descartes	2.33	92.82
MAP1B	0.0045458	64	GTEx	DepMap	Descartes	14.86	337.30
AKAP12	0.0041938	89	GTEx	DepMap	Descartes	5.64	179.79
ATP6V1B2	0.0029639	196	GTEx	DepMap	Descartes	3.97	153.53
HMGA1	0.0028138	216	GTEx	DepMap	Descartes	2.37	292.90
RBMS3	0.0025319	265	GTEx	DepMap	Descartes	2.98	102.11
BMP7	0.0023457	299	GTEx	DepMap	Descartes	0.54	36.11
SLIT3	0.0023417	301	GTEx	DepMap	Descartes	3.70	101.61
FOXO3	0.0022605	321	GTEx	DepMap	Descartes	4.27	166.32
NFIL3	0.0020770	378	GTEx	DepMap	Descartes	2.51	353.14
RGS17	0.0016233	537	GTEx	DepMap	Descartes	0.31	9.98
HK2	0.0014701	607	GTEx	DepMap	Descartes	0.48	20.86
TUBB4B	0.0012717	734	GTEx	DepMap	Descartes	3.67	484.43
HNRNPA0	0.0012237	766	GTEx	DepMap	Descartes	2.82	95.21
FAM171B	0.0012053	779	GTEx	DepMap	Descartes	0.67	37.90
TACC2	0.0011700	806	GTEx	DepMap	Descartes	0.32	8.60
CELF2	0.0011621	814	GTEx	DepMap	Descartes	2.49	90.68
AHSA1	0.0007443	1258	GTEx	DepMap	Descartes	1.64	304.13
TUBB2A	0.0006734	1381	GTEx	DepMap	Descartes	2.14	328.30
NCS1	0.0006168	1489	GTEx	DepMap	Descartes	0.48	24.68
RUFY3	0.0006141	1492	GTEx	DepMap	Descartes	1.15	74.47
TTC8	0.0004490	1867	GTEx	DepMap	Descartes	0.14	6.30
ANK2	0.0003985	2017	GTEx	DepMap	Descartes	0.90	17.58
SLC35G2	0.0003516	2146	GTEx	DepMap	Descartes	0.20	NA
TDG	0.0003141	2258	GTEx	DepMap	Descartes	0.50	43.64
STRA6	0.0002936	2324	GTEx	DepMap	Descartes	0.05	4.62
MAGI3	0.0002879	2351	GTEx	DepMap	Descartes	0.30	12.08
PTS	0.0002765	2392	GTEx	DepMap	Descartes	0.39	99.36
DNER	0.0002509	2490	GTEx	DepMap	Descartes	0.10	8.19
EXOC5	0.0002189	2617	GTEx	DepMap	Descartes	0.64	16.66

VanGroningen Mesenchymal Genes
Mesenchymal marker genes from Supplementary Table 2 of Van Groningen et al. Nature Genetics 2017. These genes were identified by differential expression analysis of mesenchymal-like and adrenergic-like neuroblastoma cell lines.
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.69e-46
Mean rank of genes in gene set: 3860.62
Median rank of genes in gene set: 1879
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
EMP1	0.0094562	2	GTEx	DepMap	Descartes	39.52	1947.89
THBS1	0.0067087	16	GTEx	DepMap	Descartes	38.07	1452.17
S1PR3	0.0066703	17	GTEx	DepMap	Descartes	6.04	202.18
EGFR	0.0065333	19	GTEx	DepMap	Descartes	6.70	211.65
FN1	0.0064211	22	GTEx	DepMap	Descartes	26.36	784.78
TNFRSF12A	0.0063445	23	GTEx	DepMap	Descartes	4.94	855.81
CFH	0.0061403	26	GTEx	DepMap	Descartes	10.59	453.59
MEST	0.0057630	31	GTEx	DepMap	Descartes	3.47	319.00
UGDH	0.0055700	34	GTEx	DepMap	Descartes	6.68	645.30
GSN	0.0055647	35	GTEx	DepMap	Descartes	22.16	1025.64
OLFML2B	0.0053294	42	GTEx	DepMap	Descartes	6.70	566.55
FGFR1	0.0052568	43	GTEx	DepMap	Descartes	11.87	555.91
TFPI	0.0051289	45	GTEx	DepMap	Descartes	5.16	196.80
TM4SF1	0.0050563	46	GTEx	DepMap	Descartes	19.49	1721.22
ANXA1	0.0049648	49	GTEx	DepMap	Descartes	19.94	2433.59
FBN2	0.0048333	54	GTEx	DepMap	Descartes	0.83	20.71
SDC2	0.0048237	55	GTEx	DepMap	Descartes	3.87	331.37
EPS8	0.0047791	56	GTEx	DepMap	Descartes	3.03	174.18
PXDC1	0.0046731	58	GTEx	DepMap	Descartes	3.65	555.38
NID1	0.0044697	70	GTEx	DepMap	Descartes	4.64	198.49
EGR3	0.0044632	71	GTEx	DepMap	Descartes	7.24	507.90
DLC1	0.0044557	72	GTEx	DepMap	Descartes	5.21	200.32
ANXA5	0.0043676	77	GTEx	DepMap	Descartes	14.01	2195.49
SASH1	0.0041263	90	GTEx	DepMap	Descartes	4.20	153.71
FZD1	0.0040583	98	GTEx	DepMap	Descartes	1.64	61.07
FAT1	0.0040044	100	GTEx	DepMap	Descartes	2.59	46.20
WWTR1	0.0039913	101	GTEx	DepMap	Descartes	3.82	190.89
SRPX	0.0039398	104	GTEx	DepMap	Descartes	5.59	797.19
CALU	0.0038769	108	GTEx	DepMap	Descartes	4.44	229.69
NR3C1	0.0036295	119	GTEx	DepMap	Descartes	6.43	240.27

Descartes adrenocortical markers
Top 50 marker genes of adrenocortical cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/adrenocortical/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.62e-02
Mean rank of genes in gene set: 4985
Median rank of genes in gene set: 4867.5
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
HMGCR	0.0037450	114	GTEx	DepMap	Descartes	1.84	114.42
LDLR	0.0034994	127	GTEx	DepMap	Descartes	3.59	182.62
HMGCS1	0.0032836	152	GTEx	DepMap	Descartes	2.09	118.81
SH3BP5	0.0026634	241	GTEx	DepMap	Descartes	2.21	182.06
MSMO1	0.0015933	546	GTEx	DepMap	Descartes	0.76	92.58
DHCR24	0.0014687	609	GTEx	DepMap	Descartes	0.41	18.77
PDE10A	0.0013690	668	GTEx	DepMap	Descartes	0.28	9.12
CLU	0.0012535	748	GTEx	DepMap	Descartes	4.97	522.94
POR	0.0007118	1310	GTEx	DepMap	Descartes	0.82	86.47
NPC1	0.0003845	2062	GTEx	DepMap	Descartes	0.56	31.01
FDXR	0.0002866	2356	GTEx	DepMap	Descartes	0.07	6.97
PAPSS2	0.0002548	2468	GTEx	DepMap	Descartes	0.52	32.52
DNER	0.0002509	2490	GTEx	DepMap	Descartes	0.10	8.19
IGF1R	0.0002015	2693	GTEx	DepMap	Descartes	0.57	12.27
FDPS	0.0001855	2774	GTEx	DepMap	Descartes	0.58	66.72
SLC1A2	0.0000877	3276	GTEx	DepMap	Descartes	0.11	2.12
FDX1	-0.0000713	4732	GTEx	DepMap	Descartes	0.24	20.63
SCAP	-0.0000724	4750	GTEx	DepMap	Descartes	0.24	14.90
SH3PXD2B	-0.0000903	4985	GTEx	DepMap	Descartes	0.25	7.03
SGCZ	-0.0000961	5079	GTEx	DepMap	Descartes	0.00	0.00
SLC16A9	-0.0001259	5562	GTEx	DepMap	Descartes	0.06	4.81
CYB5B	-0.0001300	5643	GTEx	DepMap	Descartes	0.44	30.96
STAR	-0.0001551	6042	GTEx	DepMap	Descartes	0.01	0.64
INHA	-0.0002049	6877	GTEx	DepMap	Descartes	0.00	0.00
SCARB1	-0.0002074	6924	GTEx	DepMap	Descartes	0.12	4.27
FREM2	-0.0002306	7321	GTEx	DepMap	Descartes	0.01	0.07
BAIAP2L1	-0.0002546	7662	GTEx	DepMap	Descartes	0.00	0.19
FRMD5	-0.0003021	8362	GTEx	DepMap	Descartes	0.03	1.32
APOC1	-0.0003295	8720	GTEx	DepMap	Descartes	0.22	93.75
TM7SF2	-0.0003348	8784	GTEx	DepMap	Descartes	0.02	1.85

Descartes chromaffin markers
Top 50 marker genes of chromaffin cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/chromaffin/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 8982.95
Median rank of genes in gene set: 10182
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
MAP1B	0.0045458	64	GTEx	DepMap	Descartes	14.86	337.30
RGMB	0.0011498	823	GTEx	DepMap	Descartes	0.72	36.70
TUBB2A	0.0006734	1381	GTEx	DepMap	Descartes	2.14	328.30
HS3ST5	0.0000920	3246	GTEx	DepMap	Descartes	0.02	1.09
SYNPO2	0.0000140	3849	GTEx	DepMap	Descartes	0.92	16.94
CNKSR2	-0.0000064	4018	GTEx	DepMap	Descartes	0.11	4.37
TMEM132C	-0.0001335	5710	GTEx	DepMap	Descartes	0.07	3.99
GAL	-0.0001860	6553	GTEx	DepMap	Descartes	0.04	12.62
EPHA6	-0.0002100	6964	GTEx	DepMap	Descartes	0.00	0.00
KCNB2	-0.0002211	7149	GTEx	DepMap	Descartes	0.00	0.00
PLXNA4	-0.0002401	7469	GTEx	DepMap	Descartes	0.03	0.72
ANKFN1	-0.0002605	7740	GTEx	DepMap	Descartes	0.00	0.00
EYA4	-0.0003025	8372	GTEx	DepMap	Descartes	0.01	0.17
TMEFF2	-0.0003101	8464	GTEx	DepMap	Descartes	0.01	0.70
MAB21L1	-0.0003357	8799	GTEx	DepMap	Descartes	0.13	14.89
RPH3A	-0.0003582	9080	GTEx	DepMap	Descartes	0.00	0.06
ALK	-0.0003765	9317	GTEx	DepMap	Descartes	0.00	0.13
NTRK1	-0.0003912	9506	GTEx	DepMap	Descartes	0.00	0.21
SLC44A5	-0.0004133	9742	GTEx	DepMap	Descartes	0.00	0.10
EYA1	-0.0004516	10124	GTEx	DepMap	Descartes	0.00	0.13
REEP1	-0.0004584	10182	GTEx	DepMap	Descartes	0.07	4.17
RBFOX1	-0.0004861	10422	GTEx	DepMap	Descartes	0.00	0.12
FAT3	-0.0005108	10624	GTEx	DepMap	Descartes	0.00	0.04
RYR2	-0.0005152	10657	GTEx	DepMap	Descartes	0.01	0.23
IL7	-0.0005502	10903	GTEx	DepMap	Descartes	0.01	0.62
GREM1	-0.0005557	10936	GTEx	DepMap	Descartes	0.47	4.93
NPY	-0.0005754	11061	GTEx	DepMap	Descartes	0.02	4.26
SLC6A2	-0.0005940	11151	GTEx	DepMap	Descartes	0.01	1.00
PTCHD1	-0.0006063	11218	GTEx	DepMap	Descartes	0.00	0.06
CNTFR	-0.0006128	11260	GTEx	DepMap	Descartes	0.02	1.47

Descartes Vascular_endothelial markers
Top 50 marker genes of Vascular_endothelial cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/vascular_endothelial/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.99e-01
Mean rank of genes in gene set: 8202.18
Median rank of genes in gene set: 9440
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
EFNB2	0.0037025	116	GTEx	DepMap	Descartes	2.08	111.75
CYP26B1	0.0032324	158	GTEx	DepMap	Descartes	1.21	86.23
ARHGAP29	0.0017707	479	GTEx	DepMap	Descartes	0.77	22.82
ID1	0.0009214	1034	GTEx	DepMap	Descartes	2.02	523.70
TMEM88	-0.0000090	4034	GTEx	DepMap	Descartes	0.30	83.66
CALCRL	-0.0000401	4337	GTEx	DepMap	Descartes	0.58	20.42
SHE	-0.0000507	4443	GTEx	DepMap	Descartes	0.15	5.78
EHD3	-0.0000806	4843	GTEx	DepMap	Descartes	0.09	4.57
F8	-0.0001079	5273	GTEx	DepMap	Descartes	0.06	1.53
CRHBP	-0.0001517	5988	GTEx	DepMap	Descartes	0.00	0.00
SHANK3	-0.0001785	6431	GTEx	DepMap	Descartes	0.28	7.55
NR5A2	-0.0001924	6668	GTEx	DepMap	Descartes	0.00	0.14
GALNT15	-0.0002089	6948	GTEx	DepMap	Descartes	0.05	NA
ESM1	-0.0002508	7615	GTEx	DepMap	Descartes	0.15	10.07
IRX3	-0.0003190	8576	GTEx	DepMap	Descartes	0.02	2.54
NPR1	-0.0003247	8637	GTEx	DepMap	Descartes	0.04	1.35
HYAL2	-0.0003562	9052	GTEx	DepMap	Descartes	0.14	7.99
CEACAM1	-0.0003674	9192	GTEx	DepMap	Descartes	0.02	0.79
KDR	-0.0003831	9404	GTEx	DepMap	Descartes	0.12	3.02
BTNL9	-0.0003889	9476	GTEx	DepMap	Descartes	0.01	0.19
CHRM3	-0.0003922	9515	GTEx	DepMap	Descartes	0.02	0.37
MYRIP	-0.0003960	9559	GTEx	DepMap	Descartes	0.01	0.37
CLDN5	-0.0004103	9709	GTEx	DepMap	Descartes	0.21	12.97
RASIP1	-0.0004288	9908	GTEx	DepMap	Descartes	0.08	4.02
RAMP2	-0.0005263	10743	GTEx	DepMap	Descartes	0.20	52.09
KANK3	-0.0005766	11067	GTEx	DepMap	Descartes	0.03	2.32
FLT4	-0.0006038	11207	GTEx	DepMap	Descartes	0.04	0.80
NOTCH4	-0.0006103	11247	GTEx	DepMap	Descartes	0.06	1.58
TEK	-0.0006194	11292	GTEx	DepMap	Descartes	0.01	0.25
ROBO4	-0.0006208	11303	GTEx	DepMap	Descartes	0.05	1.39

Descartes stromal markers
Top 50 marker genes of stromal cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/stromal/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.99e-02
Mean rank of genes in gene set: 5319.75
Median rank of genes in gene set: 2519
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
LUM	0.0079736	8	GTEx	DepMap	Descartes	48.81	4979.99
DCN	0.0066557	18	GTEx	DepMap	Descartes	65.98	2707.49
PAMR1	0.0053871	40	GTEx	DepMap	Descartes	2.29	159.92
OGN	0.0052020	44	GTEx	DepMap	Descartes	7.42	678.65
ISLR	0.0033808	141	GTEx	DepMap	Descartes	4.30	470.41
SCARA5	0.0029524	197	GTEx	DepMap	Descartes	1.54	111.27
BICC1	0.0028414	209	GTEx	DepMap	Descartes	1.38	65.41
PRRX1	0.0027156	236	GTEx	DepMap	Descartes	2.75	204.10
ABCA6	0.0026194	249	GTEx	DepMap	Descartes	1.27	44.05
PCOLCE	0.0024697	275	GTEx	DepMap	Descartes	5.34	912.55
CDH11	0.0021802	343	GTEx	DepMap	Descartes	1.66	59.88
COL6A3	0.0021504	353	GTEx	DepMap	Descartes	7.23	168.92
EDNRA	0.0018505	445	GTEx	DepMap	Descartes	0.98	58.96
FREM1	0.0017889	473	GTEx	DepMap	Descartes	0.36	9.17
CD248	0.0014656	612	GTEx	DepMap	Descartes	0.86	83.87
PCDH18	0.0010619	892	GTEx	DepMap	Descartes	0.52	28.29
GLI2	0.0008968	1072	GTEx	DepMap	Descartes	0.22	8.26
PDGFRA	0.0007088	1311	GTEx	DepMap	Descartes	1.19	45.86
LAMC3	0.0004293	1922	GTEx	DepMap	Descartes	0.06	2.85
COL12A1	0.0003769	2075	GTEx	DepMap	Descartes	2.10	48.96
ADAMTSL3	0.0003261	2220	GTEx	DepMap	Descartes	0.31	11.63
PRICKLE1	0.0003224	2232	GTEx	DepMap	Descartes	0.48	21.68
LOX	0.0001798	2806	GTEx	DepMap	Descartes	0.74	40.96
IGFBP3	0.0000810	3320	GTEx	DepMap	Descartes	1.25	106.10
COL1A2	0.0000118	3867	GTEx	DepMap	Descartes	31.50	1208.43
LRRC17	-0.0001086	5283	GTEx	DepMap	Descartes	0.18	18.31
GAS2	-0.0001417	5830	GTEx	DepMap	Descartes	0.01	1.08
COL27A1	-0.0004775	10340	GTEx	DepMap	Descartes	0.26	8.02
DKK2	-0.0004928	10488	GTEx	DepMap	Descartes	0.05	1.87
HHIP	-0.0005083	10599	GTEx	DepMap	Descartes	0.06	1.53

Descartes sympathoblasts markers
Top 50 marker genes of sympathoblasts cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/sympathoblasts/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.78e-01
Mean rank of genes in gene set: 7460.71
Median rank of genes in gene set: 7566
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
TIAM1	0.0048898	52	GTEx	DepMap	Descartes	2.33	92.82
CNTN3	0.0024852	271	GTEx	DepMap	Descartes	0.27	16.52
ARC	0.0008395	1136	GTEx	DepMap	Descartes	1.98	192.59
ROBO1	0.0004142	1964	GTEx	DepMap	Descartes	0.34	12.88
PENK	0.0000682	3409	GTEx	DepMap	Descartes	0.27	59.96
GRM7	0.0000236	3756	GTEx	DepMap	Descartes	0.02	1.48
SLC24A2	-0.0000274	4215	GTEx	DepMap	Descartes	0.01	0.23
GALNTL6	-0.0000338	4275	GTEx	DepMap	Descartes	0.00	0.11
CDH18	-0.0000767	4799	GTEx	DepMap	Descartes	0.01	0.32
PACRG	-0.0000783	4818	GTEx	DepMap	Descartes	0.01	2.03
SLC35F3	-0.0001140	5369	GTEx	DepMap	Descartes	0.00	0.11
SORCS3	-0.0001156	5404	GTEx	DepMap	Descartes	0.00	0.24
SPOCK3	-0.0001264	5572	GTEx	DepMap	Descartes	0.00	0.21
AGBL4	-0.0001391	5792	GTEx	DepMap	Descartes	0.00	0.24
CDH12	-0.0001665	6244	GTEx	DepMap	Descartes	0.00	0.00
EML6	-0.0001796	6448	GTEx	DepMap	Descartes	0.02	0.35
TBX20	-0.0001922	6665	GTEx	DepMap	Descartes	0.00	0.00
GRID2	-0.0002169	7081	GTEx	DepMap	Descartes	0.01	0.26
CCSER1	-0.0002420	7496	GTEx	DepMap	Descartes	0.01	NA
ST18	-0.0002528	7636	GTEx	DepMap	Descartes	0.00	0.07
KSR2	-0.0002863	8130	GTEx	DepMap	Descartes	0.00	0.05
HTATSF1	-0.0003199	8585	GTEx	DepMap	Descartes	0.42	39.28
PCSK2	-0.0003414	8858	GTEx	DepMap	Descartes	0.03	1.33
FGF14	-0.0004191	9809	GTEx	DepMap	Descartes	0.02	0.59
TENM1	-0.0004239	9857	GTEx	DepMap	Descartes	0.03	NA
MGAT4C	-0.0004360	9974	GTEx	DepMap	Descartes	0.01	0.09
SLC18A1	-0.0004488	10100	GTEx	DepMap	Descartes	0.00	0.00
DGKK	-0.0004533	10137	GTEx	DepMap	Descartes	0.00	0.03
LAMA3	-0.0004752	10316	GTEx	DepMap	Descartes	0.02	0.62
KCTD16	-0.0004958	10510	GTEx	DepMap	Descartes	0.01	0.12

Descartes erythroblasts markers
Top 50 marker genes of erythroblasts cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/erythroblasts/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.42e-01
Mean rank of genes in gene set: 6519.38
Median rank of genes in gene set: 6067
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
TSPAN5	0.0045684	62	GTEx	DepMap	Descartes	1.88	135.01
RGS6	0.0017426	492	GTEx	DepMap	Descartes	0.22	11.41
GCLC	0.0009265	1028	GTEx	DepMap	Descartes	0.55	44.55
SOX6	0.0008991	1065	GTEx	DepMap	Descartes	0.38	12.66
RAPGEF2	0.0006710	1384	GTEx	DepMap	Descartes	1.17	39.31
ANK1	0.0002225	2602	GTEx	DepMap	Descartes	0.16	4.02
SELENBP1	0.0000991	3201	GTEx	DepMap	Descartes	0.28	26.50
SLC25A21	0.0000260	3741	GTEx	DepMap	Descartes	0.00	0.22
SPECC1	-0.0000089	4033	GTEx	DepMap	Descartes	0.18	6.50
BLVRB	-0.0000626	4598	GTEx	DepMap	Descartes	0.23	44.79
FECH	-0.0000801	4838	GTEx	DepMap	Descartes	0.08	3.44
SLC4A1	-0.0001270	5588	GTEx	DepMap	Descartes	0.00	0.00
ALAS2	-0.0001333	5705	GTEx	DepMap	Descartes	0.00	0.00
RHD	-0.0001408	5819	GTEx	DepMap	Descartes	0.00	0.00
TMCC2	-0.0001566	6067	GTEx	DepMap	Descartes	0.02	1.76
ABCB10	-0.0001770	6402	GTEx	DepMap	Descartes	0.07	5.36
TRAK2	-0.0002156	7059	GTEx	DepMap	Descartes	0.23	9.71
TFR2	-0.0002649	7819	GTEx	DepMap	Descartes	0.00	0.48
SPTB	-0.0003453	8911	GTEx	DepMap	Descartes	0.01	0.08
XPO7	-0.0004391	10005	GTEx	DepMap	Descartes	0.21	11.13
DENND4A	-0.0004392	10009	GTEx	DepMap	Descartes	0.85	27.38
CAT	-0.0004519	10125	GTEx	DepMap	Descartes	0.33	38.49
CPOX	-0.0004626	10214	GTEx	DepMap	Descartes	0.07	6.41
SLC25A37	-0.0004902	10461	GTEx	DepMap	Descartes	0.61	31.66
MARCH3	-0.0005407	10838	GTEx	DepMap	Descartes	0.18	NA
MICAL2	-0.0006077	11230	GTEx	DepMap	Descartes	0.67	17.37
SNCA	-0.0006203	11300	GTEx	DepMap	Descartes	0.02	1.34
GYPC	-0.0009308	12161	GTEx	DepMap	Descartes	0.33	39.40
EPB41	-0.0010486	12305	GTEx	DepMap	Descartes	0.03	1.55
NA	NA	NA	NA	NA	NA	NA	NA

Descartes myeloid markers
Top 50 marker genes of myeloid cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/myeloid/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.69e-01
Mean rank of genes in gene set: 7369.29
Median rank of genes in gene set: 8739.5
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
TGFBI	0.0042758	83	GTEx	DepMap	Descartes	8.08	436.68
AXL	0.0018825	431	GTEx	DepMap	Descartes	1.87	111.22
CST3	0.0014059	642	GTEx	DepMap	Descartes	12.58	935.19
HRH1	0.0011427	828	GTEx	DepMap	Descartes	0.43	23.82
RGL1	0.0010648	888	GTEx	DepMap	Descartes	0.88	44.53
ABCA1	0.0005683	1576	GTEx	DepMap	Descartes	1.75	40.44
SLC1A3	0.0003329	2197	GTEx	DepMap	Descartes	0.27	15.45
IFNGR1	0.0002947	2318	GTEx	DepMap	Descartes	0.70	77.10
FGL2	0.0001624	2889	GTEx	DepMap	Descartes	0.91	65.89
SLCO2B1	0.0000965	3216	GTEx	DepMap	Descartes	0.32	14.29
LGMN	0.0000581	3480	GTEx	DepMap	Descartes	0.95	120.84
PTPRE	-0.0000198	4145	GTEx	DepMap	Descartes	0.66	29.24
MERTK	-0.0000737	4760	GTEx	DepMap	Descartes	0.07	5.31
SPP1	-0.0002365	7419	GTEx	DepMap	Descartes	0.02	1.67
CD163L1	-0.0002595	7724	GTEx	DepMap	Descartes	0.00	0.00
ITPR2	-0.0002948	8243	GTEx	DepMap	Descartes	0.37	7.54
RBPJ	-0.0003053	8406	GTEx	DepMap	Descartes	1.61	71.85
CD14	-0.0003068	8430	GTEx	DepMap	Descartes	0.34	53.62
ATP8B4	-0.0003251	8645	GTEx	DepMap	Descartes	0.02	0.95
FGD2	-0.0003393	8834	GTEx	DepMap	Descartes	0.01	0.57
SLC9A9	-0.0003519	8997	GTEx	DepMap	Descartes	0.10	8.15
ADAP2	-0.0003954	9551	GTEx	DepMap	Descartes	0.02	1.52
WWP1	-0.0004036	9647	GTEx	DepMap	Descartes	0.22	11.47
CTSB	-0.0004102	9706	GTEx	DepMap	Descartes	2.54	153.02
CPVL	-0.0004354	9964	GTEx	DepMap	Descartes	0.09	8.94
MS4A4A	-0.0004675	10254	GTEx	DepMap	Descartes	0.02	1.41
MARCH1	-0.0005106	10621	GTEx	DepMap	Descartes	0.02	NA
HCK	-0.0005211	10702	GTEx	DepMap	Descartes	0.13	10.00
CD163	-0.0005307	10779	GTEx	DepMap	Descartes	0.05	0.97
CSF1R	-0.0005416	10846	GTEx	DepMap	Descartes	0.05	2.48

Descartes Schwann markers
Top 50 marker genes of Schwann cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/schwann/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.23e-01
Mean rank of genes in gene set: 5641.86
Median rank of genes in gene set: 5641
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
LAMB1	0.0034619	131	GTEx	DepMap	Descartes	4.74	203.89
GAS7	0.0034531	133	GTEx	DepMap	Descartes	3.00	98.65
LAMC1	0.0030630	178	GTEx	DepMap	Descartes	5.54	169.45
VCAN	0.0027457	228	GTEx	DepMap	Descartes	8.44	194.54
DST	0.0020120	393	GTEx	DepMap	Descartes	4.72	57.94
VIM	0.0019695	405	GTEx	DepMap	Descartes	47.43	5041.69
SCN7A	0.0018810	433	GTEx	DepMap	Descartes	1.60	64.59
LAMA4	0.0016556	522	GTEx	DepMap	Descartes	2.61	93.16
KCTD12	0.0015100	593	GTEx	DepMap	Descartes	3.50	165.45
OLFML2A	0.0011946	792	GTEx	DepMap	Descartes	1.06	48.89
STARD13	0.0010691	886	GTEx	DepMap	Descartes	0.95	42.44
SFRP1	0.0007126	1308	GTEx	DepMap	Descartes	1.78	109.51
HMGA2	0.0006941	1343	GTEx	DepMap	Descartes	0.08	2.32
MARCKS	0.0006933	1344	GTEx	DepMap	Descartes	6.83	419.15
EDNRB	0.0006647	1394	GTEx	DepMap	Descartes	0.70	36.85
SOX5	0.0004371	1897	GTEx	DepMap	Descartes	0.17	6.41
EGFLAM	0.0003426	2166	GTEx	DepMap	Descartes	0.37	22.24
GRIK3	0.0002372	2547	GTEx	DepMap	Descartes	0.13	3.58
COL5A2	0.0001650	2880	GTEx	DepMap	Descartes	2.22	75.32
COL18A1	0.0000846	3293	GTEx	DepMap	Descartes	2.51	83.94
PLCE1	0.0000206	3781	GTEx	DepMap	Descartes	0.19	4.27
GFRA3	-0.0001294	5630	GTEx	DepMap	Descartes	0.23	33.89
IL1RAPL2	-0.0001305	5652	GTEx	DepMap	Descartes	0.00	0.11
COL25A1	-0.0001554	6053	GTEx	DepMap	Descartes	0.02	0.36
LRRTM4	-0.0002554	7672	GTEx	DepMap	Descartes	0.00	0.20
PTN	-0.0002728	7925	GTEx	DepMap	Descartes	0.27	54.82
IL1RAPL1	-0.0002780	8006	GTEx	DepMap	Descartes	0.02	1.02
TRPM3	-0.0002914	8198	GTEx	DepMap	Descartes	0.02	0.55
MPZ	-0.0002955	8254	GTEx	DepMap	Descartes	0.07	7.02
PMP22	-0.0003213	8599	GTEx	DepMap	Descartes	1.49	192.30

Descartes Megakaryocytes markers
Top 50 marker genes of Megakaryocytes cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/megakaryocytes/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.28e-01
Mean rank of genes in gene set: 7061.71
Median rank of genes in gene set: 8083
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
THBS1	0.0067087	16	GTEx	DepMap	Descartes	38.07	1452.17
GSN	0.0055647	35	GTEx	DepMap	Descartes	22.16	1025.64
LTBP1	0.0025238	268	GTEx	DepMap	Descartes	1.72	78.81
VCL	0.0021222	368	GTEx	DepMap	Descartes	2.86	89.45
MCTP1	0.0016860	510	GTEx	DepMap	Descartes	0.68	35.24
UBASH3B	0.0016804	514	GTEx	DepMap	Descartes	0.86	34.90
PDE3A	0.0015696	561	GTEx	DepMap	Descartes	0.74	25.38
ZYX	0.0012544	746	GTEx	DepMap	Descartes	3.78	441.93
STOM	0.0010760	880	GTEx	DepMap	Descartes	3.77	314.63
ITGB3	0.0009173	1041	GTEx	DepMap	Descartes	0.08	3.53
TPM4	0.0003139	2259	GTEx	DepMap	Descartes	4.16	197.28
MED12L	0.0003094	2271	GTEx	DepMap	Descartes	0.08	1.74
SLC2A3	0.0001530	2930	GTEx	DepMap	Descartes	4.96	310.26
ARHGAP6	0.0001249	3071	GTEx	DepMap	Descartes	0.11	4.78
LIMS1	0.0000614	3462	GTEx	DepMap	Descartes	1.70	93.21
TRPC6	0.0000523	3534	GTEx	DepMap	Descartes	0.05	2.92
SLC24A3	-0.0000830	4880	GTEx	DepMap	Descartes	0.04	2.04
ANGPT1	-0.0000978	5107	GTEx	DepMap	Descartes	0.11	7.10
TUBB1	-0.0001185	5457	GTEx	DepMap	Descartes	0.00	0.00
STON2	-0.0002005	6810	GTEx	DepMap	Descartes	0.05	3.23
ITGA2B	-0.0002626	7776	GTEx	DepMap	Descartes	0.01	0.65
RAP1B	-0.0002662	7837	GTEx	DepMap	Descartes	0.95	15.67
PSTPIP2	-0.0002832	8083	GTEx	DepMap	Descartes	0.05	4.28
RAB27B	-0.0002995	8319	GTEx	DepMap	Descartes	0.00	0.00
P2RX1	-0.0003564	9054	GTEx	DepMap	Descartes	0.00	0.00
HIPK2	-0.0003825	9398	GTEx	DepMap	Descartes	0.83	12.41
DOK6	-0.0004027	9637	GTEx	DepMap	Descartes	0.05	1.06
GP1BA	-0.0004858	10417	GTEx	DepMap	Descartes	0.00	0.31
ACTN1	-0.0005346	10803	GTEx	DepMap	Descartes	2.59	145.43
MMRN1	-0.0005568	10942	GTEx	DepMap	Descartes	0.06	1.45

Descartes Lyphoid markers
Top 50 marker genes of Lyphoid cells in the Decartes fetal adrenal single cell map (https://atlas.brotmanbaty.org/bbi/human-gene-expression-during-development/cell/lymphoid/in/adrenal)
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 10896.38
Median rank of genes in gene set: 12022.5
Rank on gene expression program of top 30 genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Descartes	Mean.Counts	Mean.TPM
ARID5B	0.0028071	218	GTEx	DepMap	Descartes	7.59	279.51
CELF2	0.0011621	814	GTEx	DepMap	Descartes	2.49	90.68
MBNL1	0.0000412	3618	GTEx	DepMap	Descartes	2.61	114.71
NCALD	-0.0000624	4594	GTEx	DepMap	Descartes	0.31	20.03
STK39	-0.0003657	9171	GTEx	DepMap	Descartes	0.21	18.37
ABLIM1	-0.0003682	9199	GTEx	DepMap	Descartes	1.07	35.71
BACH2	-0.0004860	10421	GTEx	DepMap	Descartes	0.24	7.08
MCTP2	-0.0005123	10636	GTEx	DepMap	Descartes	0.02	0.38
LEF1	-0.0005289	10761	GTEx	DepMap	Descartes	0.37	25.29
ITPKB	-0.0005362	10813	GTEx	DepMap	Descartes	0.61	23.66
SAMD3	-0.0005843	11108	GTEx	DepMap	Descartes	0.00	0.23
SKAP1	-0.0006168	11275	GTEx	DepMap	Descartes	0.00	0.43
DOCK10	-0.0006350	11372	GTEx	DepMap	Descartes	0.31	9.37
ETS1	-0.0006421	11401	GTEx	DepMap	Descartes	2.32	108.15
SCML4	-0.0006668	11490	GTEx	DepMap	Descartes	0.01	0.34
CCND3	-0.0006759	11530	GTEx	DepMap	Descartes	0.18	17.89
RCSD1	-0.0006960	11590	GTEx	DepMap	Descartes	0.02	1.34
TOX	-0.0007520	11760	GTEx	DepMap	Descartes	0.02	0.94
SP100	-0.0007543	11769	GTEx	DepMap	Descartes	0.85	38.89
SORL1	-0.0008039	11902	GTEx	DepMap	Descartes	0.45	10.13
RAP1GAP2	-0.0008217	11944	GTEx	DepMap	Descartes	0.01	0.44
ANKRD44	-0.0008904	12101	GTEx	DepMap	Descartes	0.08	2.88
PITPNC1	-0.0008916	12102	GTEx	DepMap	Descartes	0.41	14.33
GNG2	-0.0009220	12144	GTEx	DepMap	Descartes	0.58	39.74
BCL2	-0.0010041	12245	GTEx	DepMap	Descartes	0.26	9.24
PLEKHA2	-0.0010661	12317	GTEx	DepMap	Descartes	0.43	21.57
MSN	-0.0010887	12338	GTEx	DepMap	Descartes	1.36	90.14
PDE3B	-0.0011089	12360	GTEx	DepMap	Descartes	0.02	0.66
EVL	-0.0011242	12370	GTEx	DepMap	Descartes	0.56	37.42
ARHGAP15	-0.0011839	12400	GTEx	DepMap	Descartes	0.02	1.24

Below shows the ranks on this GEP for immune marker gene lists curated by CellTypist (https://www.celltypist.org/encyclopedia).
High ranks indicate this gene is a driver of this GEP.
These curated gene list are ranked by P-value (on this GEP) of their constituent genes.
The Mean Count column shows the mean read count in cells scoring highly (H > 50) on this gene expression program.

Fibroblasts: Fibroblasts (model markers)
the most common cells of connective tissues which synthesize the extracellular matrix and collagen to maintain tissue homeostasis:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.85e-04
Mean rank of genes in gene set: 1976.56
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
EBF2	0.0046187	61	GTEx	DepMap	Descartes	1.87	104.16
SMOC2	0.0027843	222	GTEx	DepMap	Descartes	2.98	202.82
PRRX1	0.0027156	236	GTEx	DepMap	Descartes	2.75	204.10
NTRK2	0.0013617	675	GTEx	DepMap	Descartes	1.36	46.38
SFRP1	0.0007126	1308	GTEx	DepMap	Descartes	1.78	109.51
PDGFRA	0.0007088	1311	GTEx	DepMap	Descartes	1.19	45.86
F10	0.0007054	1316	GTEx	DepMap	Descartes	0.18	35.02
OLFML1	0.0004582	1837	GTEx	DepMap	Descartes	0.42	38.32
ANGPTL1	-0.0005382	10823	GTEx	DepMap	Descartes	0.15	9.75

Macrophages: Kupffer cells (model markers)
resident macrophages in liver under non-inflammatory conditions which line the hepatic sinusoids and are involved in erythrocyte clearance:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.84e-02
Mean rank of genes in gene set: 2492.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
EGR2	0.0019928	400	GTEx	DepMap	Descartes	2.14	203.49
ARRDC3	0.0018634	438	GTEx	DepMap	Descartes	2.00	142.61
PENK	0.0000682	3409	GTEx	DepMap	Descartes	0.27	59.96
CD5L	-0.0001347	5724	GTEx	DepMap	Descartes	0.00	0.00

DC: Transitional DC (curated markers)
immature dendritic cells which follow dendritic cell precursors and are committed to the mature dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.89e-02
Mean rank of genes in gene set: 954.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
AXL	0.0018825	431	GTEx	DepMap	Descartes	1.87	111.22
KLF4	0.0006225	1478	GTEx	DepMap	Descartes	4.80	494.47

Monocyte precursor: Monocyte precursor (curated markers)
monocyte precursors that are committed to the monocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.75e-02
Mean rank of genes in gene set: 228
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
VCAN	0.0027457	228	GTEx	DepMap	Descartes	8.44	194.54

B-cell lineage: Small pre-B cells (curated markers)
non-proliferative B lymphocyte precursors derived from Pro-B cells and expressing membrane μ chains with surrogate light chains in their receptors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.77e-02
Mean rank of genes in gene set: 1129
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MME	0.0008453	1129	GTEx	DepMap	Descartes	0.36	13.42

B-cell lineage: Pro-B cells (curated markers)
early B lymphocyte progenitors undergoing D-J joining on the H chain chromosome and joining of a V segment to the rearranged D-J:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.77e-02
Mean rank of genes in gene set: 1129
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MME	0.0008453	1129	GTEx	DepMap	Descartes	0.36	13.42

ETP: ETP (curated markers)
early thymic progenitors migrated to the thymus before turning into double-negative thymocytes or other cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.06e-01
Mean rank of genes in gene set: 3071.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD34	0.0022136	333	GTEx	DepMap	Descartes	1.81	55.04
SPINK2	-0.0001403	5810	GTEx	DepMap	Descartes	0.00	0.00

Megakaryocyte precursor: Megakaryocyte precursor (model markers)
megakaryocyte precursors in the bone marrow that are committed to megakaryocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.15e-01
Mean rank of genes in gene set: 3204
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ADRA2A	0.0011825	799	GTEx	DepMap	Descartes	0.46	38.96
SLC10A5	-0.0001285	5609	GTEx	DepMap	Descartes	0.01	0.62

T cells: Tem/Effector helper T cells PD1+ (model markers)
CD4+ helper T lymphocyte subpopulation in the thymus which features the expression of programmed cell death protein 1 (PD-1):
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.19e-01
Mean rank of genes in gene set: 3255.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
INPP5J	0.0001462	2960	GTEx	DepMap	Descartes	0.07	5.48
PANX2	0.0000501	3551	GTEx	DepMap	Descartes	0.03	3.15

B cells: Transitional B cells (curated markers)
immature B cell precursors in the bone marrow which connect Pre-B cells with mature naive B cells and are subject to the process of B cell selection:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.36e-01
Mean rank of genes in gene set: 2300
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MYO1C	0.0003015	2300	GTEx	DepMap	Descartes	1.19	67.52

ILC: ILC2 (model markers)
innate lymphoid cell subpopulation II that promotes type 2 inflammation and is involved in immune response against large extracellular pathogens:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.42e-01
Mean rank of genes in gene set: 2394
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IL17RB	0.000276	2394	GTEx	DepMap	Descartes	0.03	3.45

HSC/MPP: HSC/MPP (curated markers)
hematopoietic stem cells and multipotent progenitor cells with the potential of differentiating into different blood cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.43e-01
Mean rank of genes in gene set: 4043.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD34	0.0022136	333	GTEx	DepMap	Descartes	1.81	55.04
SPINK2	-0.0001403	5810	GTEx	DepMap	Descartes	0.00	0.00
CRHBP	-0.0001517	5988	GTEx	DepMap	Descartes	0.00	0.00

MNP: MNP (curated markers)
mononuclear phagocytes including dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.75e-01
Mean rank of genes in gene set: 2889
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FGL2	0.0001624	2889	GTEx	DepMap	Descartes	0.91	65.89

Epithelial cells: Epithelial cells (curated markers)
highly specialised cells which are located in the outer layer of stroma and form the cellular components of the epithelium:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.90e-01
Mean rank of genes in gene set: 3091
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
KRT17	0.0001196	3091	GTEx	DepMap	Descartes	0.04	5.02

Plasma cells: Plasma cells (curated markers)
B-lymphocyte white blood cells capable of secreting large quantities of immunoglobulins or antibodies:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.02e-01
Mean rank of genes in gene set: 4137.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
XBP1	0.0031055	172	GTEx	DepMap	Descartes	4.14	507.71
MZB1	-0.0002841	8103	GTEx	DepMap	Descartes	0.00	NA

T cells: Memory CD4+ cytotoxic T cells (model markers)
CD4+ memory T cells which have cytotoxic activities by secreting granzymes and perforin and by killing target cells on the basis of MHC class II:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.14e-01
Mean rank of genes in gene set: 3409
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PENK	6.82e-05	3409	GTEx	DepMap	Descartes	0.27	59.96

Macrophages: Hofbauer cells (model markers)
primitive placental resident macrophages with granules and vacuoles found in placenta particularly during early pregnancy:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.26e-01
Mean rank of genes in gene set: 3551
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PANX2	5.01e-05	3551	GTEx	DepMap	Descartes	0.03	3.15

ETP: ETP (model markers)
early thymic progenitors migrated to the thymus before turning into double-negative thymocytes or other cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.38e-01
Mean rank of genes in gene set: 4451
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PENK	0.0000682	3409	GTEx	DepMap	Descartes	0.27	59.96
WDR49	-0.0001215	5493	GTEx	DepMap	Descartes	0.00	0.07

ILC: NK cells (model markers)
granular lymphocytes that play protective roles against both infectious pathogens and cancer using antibody-dependent cell-mediated cytotoxicity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.40e-01
Mean rank of genes in gene set: 5309.29
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
HMOX1	0.0085386	5	GTEx	DepMap	Descartes	17.55	3322.44
IGFBP4	0.0018728	437	GTEx	DepMap	Descartes	10.20	1054.91
CST3	0.0014059	642	GTEx	DepMap	Descartes	12.58	935.19
SPINK2	-0.0001403	5810	GTEx	DepMap	Descartes	0.00	0.00
MALAT1	-0.0002061	6903	GTEx	DepMap	Descartes	100.78	3057.43
KLHL23	-0.0005465	10879	GTEx	DepMap	Descartes	0.09	5.98
STMN1	-0.0014764	12489	GTEx	DepMap	Descartes	0.61	52.19

Granulocytes: Granulocytes (model markers)
cells of innate immune system with specific granules in the cytoplasm including neutrophils:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.68e-01
Mean rank of genes in gene set: 4689.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MAK	0.0000217	3770	GTEx	DepMap	Descartes	0.05	2.91
SLC10A5	-0.0001285	5609	GTEx	DepMap	Descartes	0.01	0.62

Plasma cells: Plasma cells (model markers)
B-lymphocyte white blood cells capable of secreting large quantities of immunoglobulins or antibodies:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.68e-01
Mean rank of genes in gene set: 5155
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
XBP1	0.0031055	172	GTEx	DepMap	Descartes	4.14	507.71
TGFBR3L	-0.0000702	4709	GTEx	DepMap	Descartes	0.00	NA
ST18	-0.0002528	7636	GTEx	DepMap	Descartes	0.00	0.07
MZB1	-0.0002841	8103	GTEx	DepMap	Descartes	0.00	NA

Monocytes: Non-classical monocytes (model markers)
CD16+ myeloid mononuclear recirculating leukocytes that are capable of differentiating into macrophages and myeloid lineage dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.73e-01
Mean rank of genes in gene set: 5182.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
BAG3	0.0023396	302	GTEx	DepMap	Descartes	6.83	700.01
RHOC	0.0005107	1703	GTEx	DepMap	Descartes	1.13	116.47
CUX2	-0.0002495	7595	GTEx	DepMap	Descartes	0.00	0.00
ABI3	-0.0005889	11131	GTEx	DepMap	Descartes	0.04	4.26

DC: DC2 (model markers)
conventional type 2 dendritic cells which constitute the major subset of myeloid dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.82e-01
Mean rank of genes in gene set: 5071
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CST3	0.0014059	642	GTEx	DepMap	Descartes	12.58	935.19
AGRP	-0.0000550	4508	GTEx	DepMap	Descartes	0.00	0.00
MMP9	-0.0004452	10063	GTEx	DepMap	Descartes	0.01	1.37

Fibroblasts: Fibroblasts (curated markers)
the most common cells of connective tissues which synthesize the extracellular matrix and collagen to maintain tissue homeostasis:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.85e-01
Mean rank of genes in gene set: 5088.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
DCN	0.0066557	18	GTEx	DepMap	Descartes	65.98	2707.49
COL1A2	0.0000118	3867	GTEx	DepMap	Descartes	31.50	1208.43
COL1A1	-0.0006378	11381	GTEx	DepMap	Descartes	56.71	2008.04

Granulocytes: Neutrophils (curated markers)
the most abundant type of granulocytes that contains distinctive cytoplasmic granules and forms an essential part of the innate immune system:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.92e-01
Mean rank of genes in gene set: 4292
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
LCN2	-3.62e-05	4292	GTEx	DepMap	Descartes	0	0.53

T cells: Follicular helper T cells (model markers)
specialised CD4+ T cell subpopulation found in the periphery within B cell follicles and stimulating the cognate follicular B cells for class-switching:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.94e-01
Mean rank of genes in gene set: 5292
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CH25H	0.0019295	416	GTEx	DepMap	Descartes	0.38	67.86
SOCS3	0.0003625	2119	GTEx	DepMap	Descartes	7.67	868.25
HOXB5	-0.0002117	6994	GTEx	DepMap	Descartes	0.01	0.80
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60

pDC precursor: pDC precursor (model markers)
precursors of plasmacytoid dendritic cells which have intermixed lymphoid and myeloid origins and give rise to plasmacytoid dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.13e-01
Mean rank of genes in gene set: 4508
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
AGRP	-5.5e-05	4508	GTEx	DepMap	Descartes	0	0

B cells: Germinal center B cells (model markers)
proliferating mature B cells that undergo somatic hypermutation and class-switch recombination in secondary lymphoid organs:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.34e-01
Mean rank of genes in gene set: 5176.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
BMP3	-0.0000711	4727	GTEx	DepMap	Descartes	0.00	0.14
AICDA	-0.0001293	5626	GTEx	DepMap	Descartes	0.01	0.79

ILC precursor: ILC precursor (model markers)
innate lymphoid cell precursors which give rise to innate lymphoid cells and lack the characteristics of their mature progenies:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.05e-01
Mean rank of genes in gene set: 5773
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PDZK1	0.0000272	3723	GTEx	DepMap	Descartes	0.01	0.81
HUNK	-0.0001773	6408	GTEx	DepMap	Descartes	0.03	0.94
HPN	-0.0002230	7188	GTEx	DepMap	Descartes	0.00	0.27

DC: Migratory DCs (model markers)
migratory dendritic cells that transport antigens to the draining lymph nodes during both homeostatic conditions and infections:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.15e-01
Mean rank of genes in gene set: 5493
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
WDR49	-0.0001215	5493	GTEx	DepMap	Descartes	0	0.07

Early MK: Early MK (model markers)
early megakaryocytes which are committed to megakaryocyte lineage cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.19e-01
Mean rank of genes in gene set: 5750
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CCDC175	-0.0001221	5502	GTEx	DepMap	Descartes	0	NA
CD207	-0.0001523	5998	GTEx	DepMap	Descartes	0	0

pDC precursor: pDC precursor (curated markers)
precursors of plasmacytoid dendritic cells which have intermixed lymphoid and myeloid origins and give rise to plasmacytoid dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.30e-01
Mean rank of genes in gene set: 5822.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CCDC50	0.0004468	1874	GTEx	DepMap	Descartes	0.86	26.30
IRF7	-0.0004155	9771	GTEx	DepMap	Descartes	0.27	30.94

Cycling cells: Cycling NK cells (model markers)
proliferating natural killer cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.34e-01
Mean rank of genes in gene set: 5849.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
KL	0.0003350	2191	GTEx	DepMap	Descartes	0.06	3.18
GRIN1	-0.0003915	9508	GTEx	DepMap	Descartes	0.00	0.00

ILC: ILC (curated markers)
specialised innate immune cells from the lymphoid lineage but without antigen-specific T-cell receptors on the surface:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.38e-01
Mean rank of genes in gene set: 5950.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
S100A13	0.0005739	1565	GTEx	DepMap	Descartes	0.64	77.33
TLE1	-0.0001611	6153	GTEx	DepMap	Descartes	0.54	33.83
AREG	-0.0004532	10133	GTEx	DepMap	Descartes	0.09	15.23

B-cell lineage: Large pre-B cells (curated markers)
proliferative B lymphocyte precursors derived from Pro-B cells and expressing membrane μ chains with surrogate light chains in their receptors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.60e-01
Mean rank of genes in gene set: 6019
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MME	0.0008453	1129	GTEx	DepMap	Descartes	0.36	13.42
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13

Cycling cells: Cycling T cells (model markers)
proliferating T lymphocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.70e-01
Mean rank of genes in gene set: 5998
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD207	-0.0001523	5998	GTEx	DepMap	Descartes	0	0

ILC: ILC3 (model markers)
innate lymphoid cell subpopulation III that is required for host defense against specific extracellular bacteria and fungi:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.70e-01
Mean rank of genes in gene set: 5998
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD207	-0.0001523	5998	GTEx	DepMap	Descartes	0	0

Promyelocytes: Promyelocytes (model markers)
early granulocyte progenitors in the bone marrow which are derived from the myeloblasts and develop into the myelocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.70e-01
Mean rank of genes in gene set: 5998
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD207	-0.0001523	5998	GTEx	DepMap	Descartes	0	0

Mono-mac: Mono-mac (curated markers)
mononuclear phagocytes including monocytes and macrophages that are involved in the regulation of innate and adaptive immunity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.72e-01
Mean rank of genes in gene set: 6095
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
HMOX1	0.0085386	5	GTEx	DepMap	Descartes	17.55	3322.44
TYROBP	-0.0009516	12185	GTEx	DepMap	Descartes	0.17	48.35

ILC: CD16+ NK cells (model markers)
CD16+ granular lymphocytes that play protective roles against both infectious pathogens and cancer using antibody-dependent cell-mediated cytotoxicity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.88e-01
Mean rank of genes in gene set: 6228
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IGFBP7	0.0024366	285	GTEx	DepMap	Descartes	60.95	10781.25
ADAMTS1	0.0007476	1251	GTEx	DepMap	Descartes	4.44	256.99
PRSS57	-0.0001244	5530	GTEx	DepMap	Descartes	0.00	0.00
FCER1G	-0.0007977	11889	GTEx	DepMap	Descartes	0.08	20.36
TYROBP	-0.0009516	12185	GTEx	DepMap	Descartes	0.17	48.35

Macrophages: Hofbauer cells (curated markers)
primitive placental resident macrophages with granules and vacuoles found in placenta particularly during early pregnancy:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.04e-01
Mean rank of genes in gene set: 6307
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SCIN	-0.0001703	6307	GTEx	DepMap	Descartes	0.05	1.85

Erythroid: Late erythroid (model markers)
late erythroid cells following early and middle erythroid cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.10e-01
Mean rank of genes in gene set: 6341.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD207	-0.0001523	5998	GTEx	DepMap	Descartes	0	0
SLC12A3	-0.0001931	6685	GTEx	DepMap	Descartes	0	0

Cycling cells: Cycling DCs (model markers)
proliferating dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.12e-01
Mean rank of genes in gene set: 6379
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MMP12	-0.0001749	6379	GTEx	DepMap	Descartes	0	0

Macrophages: Macrophages (model markers)
specialised mononuclear phagocytic cells which recognise:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.29e-01
Mean rank of genes in gene set: 6405.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FTH1	0.0009648	982	GTEx	DepMap	Descartes	30.22	6676.34
CD5L	-0.0001347	5724	GTEx	DepMap	Descartes	0.00	0.00
APOC1	-0.0003295	8720	GTEx	DepMap	Descartes	0.22	93.75
APOE	-0.0004604	10196	GTEx	DepMap	Descartes	3.33	749.15

B cells: Naive B cells (model markers)
mature B lymphocytes which express cell-surface IgM and IgD and have not been exposed to/activated by antigens:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.38e-01
Mean rank of genes in gene set: 6520
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
YBX3	0.0016410	525	GTEx	DepMap	Descartes	3.99	NA
CD74	-0.0016569	12515	GTEx	DepMap	Descartes	3.79	211.59

pDC: pDC (model markers)
rare plasmacytoid dendritic cell subpopulation which serves as the major source of type I interferons when the body is infected by a virus:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.76e-01
Mean rank of genes in gene set: 6765
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
TRIM71	0.0000354	3654	GTEx	DepMap	Descartes	0	0.08
OGDHL	-0.0004262	9876	GTEx	DepMap	Descartes	0	0.00

Erythroid: Early erythroid (model markers)
early erythroid cells which are committed to erythroid lineage cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.95e-01
Mean rank of genes in gene set: 6710.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PRSS57	-0.0001244	5530	GTEx	DepMap	Descartes	0.00	0.00
CD207	-0.0001523	5998	GTEx	DepMap	Descartes	0.00	0.00
FAM178B	-0.0001879	6595	GTEx	DepMap	Descartes	0.00	0.00
APOC1	-0.0003295	8720	GTEx	DepMap	Descartes	0.22	93.75

Early MK: Early MK (curated markers)
early megakaryocytes which are committed to megakaryocyte lineage cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.02e-01
Mean rank of genes in gene set: 7211
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CMTM5	-0.0002241	7211	GTEx	DepMap	Descartes	0.01	1.57

Double-positive thymocytes: Double-positive thymocytes (model markers)
immature thymocytes capable of binding MHC class I or II in the double positive (co-receptors CD4+ and CD8+) stage:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.06e-01
Mean rank of genes in gene set: 6670.83
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ELOVL4	0.0001963	2725	GTEx	DepMap	Descartes	0.17	13.71
RAG2	0.0000529	3527	GTEx	DepMap	Descartes	0.00	0.50
SMPD3	0.0000181	3801	GTEx	DepMap	Descartes	0.09	4.64
ARPP21	-0.0002810	8052	GTEx	DepMap	Descartes	0.00	0.03
SH3TC1	-0.0003895	9484	GTEx	DepMap	Descartes	0.01	0.31
CD52	-0.0012774	12436	GTEx	DepMap	Descartes	0.01	4.64

B-cell lineage: Small pre-B cells (model markers)
non-proliferative B lymphocyte precursors derived from Pro-B cells and expressing membrane μ chains with surrogate light chains in their receptors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.09e-01
Mean rank of genes in gene set: 6776
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
AICDA	-0.0001293	5626	GTEx	DepMap	Descartes	0.01	0.79
CD207	-0.0001523	5998	GTEx	DepMap	Descartes	0.00	0.00
TLDC2	-0.0001558	6056	GTEx	DepMap	Descartes	0.00	NA
CD79B	-0.0003847	9424	GTEx	DepMap	Descartes	0.01	1.31

Epithelial cells: Epithelial cells (model markers)
highly specialised cells which are located in the outer layer of stroma and form the cellular components of the epithelium:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.18e-01
Mean rank of genes in gene set: 7045
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CDH3	-0.0001639	6196	GTEx	DepMap	Descartes	0.01	0.67
WFDC2	-0.0002703	7894	GTEx	DepMap	Descartes	0.01	2.31

Mono-mac: Mono-mac (model markers)
mononuclear phagocytes including monocytes and macrophages that are involved in the regulation of innate and adaptive immunity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.38e-01
Mean rank of genes in gene set: 6916.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FTH1	0.0009648	982	GTEx	DepMap	Descartes	30.22	6676.34
S100A2	0.0001866	2771	GTEx	DepMap	Descartes	0.02	4.11
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60
SAT1	-0.0010291	12274	GTEx	DepMap	Descartes	3.25	686.27

T cells: Type 1 helper T cells (model markers)
CD4+ helper T lymphocyte subpopulation which is capable of producing interferon-gamma and modulating cell-mediated immune responses:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.42e-01
Mean rank of genes in gene set: 6862.2
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ANXA1	0.0049648	49	GTEx	DepMap	Descartes	19.94	2433.59
CEBPB	0.0037858	112	GTEx	DepMap	Descartes	11.27	1733.94
PRDM16	-0.0004481	10092	GTEx	DepMap	Descartes	0.03	0.69
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60
CCL5	-0.0012415	12419	GTEx	DepMap	Descartes	0.07	8.86

HSC/MPP: Neutrophil-myeloid progenitor (model markers)
progenitors of neutrophils and myeloid cells which are transitioned from hematopoietic stem cells and multipotent progenitors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.42e-01
Mean rank of genes in gene set: 7595
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CUX2	-0.0002495	7595	GTEx	DepMap	Descartes	0	0

HSC/MPP: CMP (model markers)
highly proliferative common myeloid progenitors which later give rise to granulocyte-monocyte progenitors and megakaryocyte-erythroid progenitors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.44e-01
Mean rank of genes in gene set: 7220.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD207	-0.0001523	5998	GTEx	DepMap	Descartes	0	0.00
CPA3	-0.0003076	8443	GTEx	DepMap	Descartes	0	1.84

HSC/MPP: Early lymphoid/T lymphoid (model markers)
early lymphoid/T lymphocytes with lymphocyte potential in the fetal liver before T cells emerged from the thymus:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.46e-01
Mean rank of genes in gene set: 7636
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ST18	-0.0002528	7636	GTEx	DepMap	Descartes	0	0.07

T cells: Tcm/Naive helper T cells (model markers)
CD4+ helper T lymphocytes mainly localized in secondary lymphoid tissues and sustaining the responses by proliferating and producing new effectors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.49e-01
Mean rank of genes in gene set: 6968
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CTSL	0.0055010	37	GTEx	DepMap	Descartes	10.97	NA
FCGRT	0.0001273	3060	GTEx	DepMap	Descartes	1.46	137.28
LTB	-0.0009912	12238	GTEx	DepMap	Descartes	0.01	2.12
TMSB10	-0.0019718	12537	GTEx	DepMap	Descartes	7.60	3970.03

DC: DC3 (model markers)
a dendritic cell subtype found in bone marrow which shares features with monocytes and conventional type 2 dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.56e-01
Mean rank of genes in gene set: 7301.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
BMP3	-0.0000711	4727	GTEx	DepMap	Descartes	0	0.14
OGDHL	-0.0004262	9876	GTEx	DepMap	Descartes	0	0.00

Megakaryocytes/platelets: Megakaryocytes/platelets (curated markers)
large multinucleated cells with numerous azurophilic granules and with mature blood platelets released from the cytoplasm:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.83e-01
Mean rank of genes in gene set: 7493.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CMTM5	-0.0002241	7211	GTEx	DepMap	Descartes	0.01	1.57
ITGA2B	-0.0002626	7776	GTEx	DepMap	Descartes	0.01	0.65

B-cell lineage: Large pre-B cells (model markers)
proliferative B lymphocyte precursors derived from Pro-B cells and expressing membrane μ chains with surrogate light chains in their receptors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.84e-01
Mean rank of genes in gene set: 8006
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IL1RAPL1	-0.000278	8006	GTEx	DepMap	Descartes	0.02	1.02

DC: DC1 (model markers)
conventional type 1 dendritic cells which constitute a rare dendritic cell population with superior cross-presentation ability:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.84e-01
Mean rank of genes in gene set: 8006
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IL1RAPL1	-0.000278	8006	GTEx	DepMap	Descartes	0.02	1.02

Erythrocytes: Erythrocytes (model markers)
biconcave enucleated red blood cells filled with hemoglobin to transport oxygen and carbon dioxide between the lungs and tissues:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.84e-01
Mean rank of genes in gene set: 8006
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IL1RAPL1	-0.000278	8006	GTEx	DepMap	Descartes	0.02	1.02

Endothelial cells: Endothelial cells (curated markers)
single-layered lining cells that constitute the interior vasculature:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.85e-01
Mean rank of genes in gene set: 7284.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SPARCL1	0.0025169	269	GTEx	DepMap	Descartes	6.99	610.80
CLDN5	-0.0004103	9709	GTEx	DepMap	Descartes	0.21	12.97
PLVAP	-0.0007936	11875	GTEx	DepMap	Descartes	0.25	14.38

Monocyte precursor: Monocyte precursor (model markers)
monocyte precursors that are committed to the monocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.00e-01
Mean rank of genes in gene set: 7615.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CUX2	-0.0002495	7595	GTEx	DepMap	Descartes	0	0.00
ST18	-0.0002528	7636	GTEx	DepMap	Descartes	0	0.07

HSC/MPP: HSC/MPP (model markers)
hematopoietic stem cells and multipotent progenitor cells with the potential of differentiating into different blood cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.06e-01
Mean rank of genes in gene set: 7659
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SPINK2	-0.0001403	5810	GTEx	DepMap	Descartes	0	0
GRIN1	-0.0003915	9508	GTEx	DepMap	Descartes	0	0

Macrophages: Kupffer cells (curated markers)
resident macrophages in liver under non-inflammatory conditions which line the hepatic sinusoids and are involved in erythrocyte clearance:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.07e-01
Mean rank of genes in gene set: 7667
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD5L	-0.0001347	5724	GTEx	DepMap	Descartes	0.00	0.00
VCAM1	-0.0004003	9610	GTEx	DepMap	Descartes	0.57	27.59

Monocytes: Classical monocytes (curated markers)
CD14+ myeloid mononuclear recirculating leukocytes that are capable of differentiating into macrophages and myeloid lineage dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.24e-01
Mean rank of genes in gene set: 8430
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD14	-0.0003068	8430	GTEx	DepMap	Descartes	0.34	53.62

Mast cells: Mast cells (model markers)
long-lived innate migrant cells found in most tissues with many large basophilic granules rich in histamine and heparin:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.25e-01
Mean rank of genes in gene set: 8443
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CPA3	-0.0003076	8443	GTEx	DepMap	Descartes	0	1.84

T cells: Regulatory T cells (curated markers)
T cell subpopulation which modulates immune responses and regulates other cells through direct cell-cell contact and cytokine release:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.31e-01
Mean rank of genes in gene set: 8501
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FOXP3	-0.0003128	8501	GTEx	DepMap	Descartes	0.01	1.74

ILC: CD16- NK cells (curated markers)
CD16- granular lymphocytes that play protective roles against both infectious pathogens and cancer using antibody-dependent cell-mediated cytotoxicity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.39e-01
Mean rank of genes in gene set: 8589
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD160	-0.0003202	8589	GTEx	DepMap	Descartes	0	0.03

Double-positive thymocytes: Double-positive thymocytes (curated markers)
immature thymocytes capable of binding MHC class I or II in the double positive (co-receptors CD4+ and CD8+) stage:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.50e-01
Mean rank of genes in gene set: 8001.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SMPD3	0.0000181	3801	GTEx	DepMap	Descartes	0.09	4.64
CD8A	-0.0009653	12202	GTEx	DepMap	Descartes	0.01	1.15

T cells: Tem/Effector helper T cells (curated markers)
CD4+ helper T lymphocytes mainly localized in lymphoid and peripheral tissues and presenting an immediate:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.63e-01
Mean rank of genes in gene set: 7769
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ITGB1	0.0009554	992	GTEx	DepMap	Descartes	2.33	158.73
AQP3	-0.0005170	10676	GTEx	DepMap	Descartes	0.08	5.55
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60

Mast cells: Mast cells (curated markers)
long-lived innate migrant cells found in most tissues with many large basophilic granules rich in histamine and heparin:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.66e-01
Mean rank of genes in gene set: 8136.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
TPSB2	-0.0002656	7830	GTEx	DepMap	Descartes	0.01	3.03
CPA3	-0.0003076	8443	GTEx	DepMap	Descartes	0.00	1.84

T cells: Type 17 helper T cells (model markers)
CD4+ helper T lymphocyte subpopulation which is capable of producing interleukin 17 (IL-17) and mediating protective immunity and autoimmunity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.89e-01
Mean rank of genes in gene set: 7954.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ANXA1	0.0049648	49	GTEx	DepMap	Descartes	19.94	2433.59
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60
S100A4	-0.0009428	12175	GTEx	DepMap	Descartes	0.59	231.32

Cycling cells: Cycling B cells (model markers)
proliferating B lymphocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.00e-01
Mean rank of genes in gene set: 8037
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes
CD207	-0.0001523	5998	GTEx	DepMap	Descartes
RGS13	-0.0003215	8605	GTEx	DepMap	Descartes
GRIN1	-0.0003915	9508	GTEx	DepMap	Descartes

DC: DC1 (curated markers)
conventional type 1 dendritic cells which constitute a rare dendritic cell population with superior cross-presentation ability:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.06e-01
Mean rank of genes in gene set: 8483
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
BATF3	-0.0000582	4543	GTEx	DepMap	Descartes	0.15	15.22
CADM1	-0.0012534	12423	GTEx	DepMap	Descartes	0.10	2.46

pDC: pDC (curated markers)
rare plasmacytoid dendritic cell subpopulation which serves as the major source of type I interferons when the body is infected by a virus:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.22e-01
Mean rank of genes in gene set: 9612
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PLD4	-0.0004006	9612	GTEx	DepMap	Descartes	0.01	0.69

Myelocytes: Myelocytes (model markers)
early granulocyte precursors that are derive from promyelocytes and later mature into metamyelocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.23e-01
Mean rank of genes in gene set: 7778.2
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
S100A6	0.0012974	712	GTEx	DepMap	Descartes	7.15	2882.33
FTH1	0.0009648	982	GTEx	DepMap	Descartes	30.22	6676.34
TYROBP	-0.0009516	12185	GTEx	DepMap	Descartes	0.17	48.35
ACTB	-0.0013873	12468	GTEx	DepMap	Descartes	15.34	1760.45
SRGN	-0.0020768	12544	GTEx	DepMap	Descartes	2.32	287.99

B-cell lineage: Pre-pro-B cells (curated markers)
the earliest primitive B cell progenitors which express CD34 and SPINK2:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.25e-01
Mean rank of genes in gene set: 8666.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ZCCHC7	-0.0000841	4895	GTEx	DepMap	Descartes	0.15	12.97
IL7R	-0.0012827	12438	GTEx	DepMap	Descartes	0.09	4.94

ILC: ILC3 (curated markers)
innate lymphoid cell subpopulation III that is required for host defense against specific extracellular bacteria and fungi:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.33e-01
Mean rank of genes in gene set: 8299.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
RORC	-0.0001245	5535	GTEx	DepMap	Descartes	0.00	0.25
KIT	-0.0003993	9599	GTEx	DepMap	Descartes	0.03	0.99
IL4I1	-0.0004148	9764	GTEx	DepMap	Descartes	0.01	0.40

Double-negative thymocytes: Double-negative thymocytes (curated markers)
the early immature thymocytes from the thymus in the double negative (co-receptors CD4- and CD8-) stage:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.36e-01
Mean rank of genes in gene set: 8778
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FXYD2	-0.0001321	5676	GTEx	DepMap	Descartes	0.00	0.17
HES1	-0.0007954	11880	GTEx	DepMap	Descartes	2.88	519.69

DC precursor: DC precursor (model markers)
dendritic cell precursors that differentiate into dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.40e-01
Mean rank of genes in gene set: 9876
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
OGDHL	-0.0004262	9876	GTEx	DepMap	Descartes	0	0

Megakaryocyte precursor: Megakaryocyte precursor (curated markers)
megakaryocyte precursors in the bone marrow that are committed to megakaryocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.42e-01
Mean rank of genes in gene set: 8848
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PRSS57	-0.0001244	5530	GTEx	DepMap	Descartes	0.00	0.00
GATA2	-0.0009356	12166	GTEx	DepMap	Descartes	0.08	4.17

B cells: Germinal center B cells (curated markers)
proliferating mature B cells that undergo somatic hypermutation and class-switch recombination in secondary lymphoid organs:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.77e-01
Mean rank of genes in gene set: 9250
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
POU2AF1	-0.0002716	7912	GTEx	DepMap	Descartes	0.01	0.26
CD40	-0.0005067	10588	GTEx	DepMap	Descartes	0.15	20.15

B cells: Follicular B cells (curated markers)
resting mature B lymphocytes found in the primary and secondary lymphoid follicles and participating in T cell-dependent immune responses:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.91e-01
Mean rank of genes in gene set: 9424.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CXCR5	-0.0003815	9384	GTEx	DepMap	Descartes	0.01	0.69
CD22	-0.0003879	9465	GTEx	DepMap	Descartes	0.01	0.75

Monocytes: Monocytes (model markers)
myeloid mononuclear recirculating leukocytes that are capable of differentiating into macrophages and myeloid lineage dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.00e-01
Mean rank of genes in gene set: 8960
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
NEAT1	0.0002688	2421	GTEx	DepMap	Descartes	34.42	423.38
TYROBP	-0.0009516	12185	GTEx	DepMap	Descartes	0.17	48.35
SAT1	-0.0010291	12274	GTEx	DepMap	Descartes	3.25	686.27

B cells: B cells (curated markers)
B lymphocytes with diverse cell surface immunoglobulin receptors recognising specific antigenic epitopes and mediating humoral immunity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.04e-01
Mean rank of genes in gene set: 9623
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD19	-0.0003262	8675	GTEx	DepMap	Descartes	0.00	0.0
CD79A	-0.0005041	10571	GTEx	DepMap	Descartes	0.01	2.2

Endothelial cells: Endothelial cells (model markers)
single-layered lining cells that constitute the interior vasculature:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.05e-01
Mean rank of genes in gene set: 8653.25
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
JAM2	0.0021738	345	GTEx	DepMap	Descartes	1.08	71.74
RAMP3	-0.0006045	11211	GTEx	DepMap	Descartes	0.03	2.98
MMRN2	-0.0006649	11480	GTEx	DepMap	Descartes	0.09	4.42
VWF	-0.0006910	11577	GTEx	DepMap	Descartes	0.64	9.07

T cells: MAIT cells (curated markers)
mucosal-associated invariant T cells which have semi-invariant T-cell receptors and are restricted by the MHC I-like molecule MR1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.07e-01
Mean rank of genes in gene set: 9662
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SLC4A10	-0.0002562	7685	GTEx	DepMap	Descartes	0.00	0.06
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60

Granulocytes: Granulocytes (curated markers)
cells of innate immune system with specific granules in the cytoplasm including neutrophils:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.12e-01
Mean rank of genes in gene set: 9098.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CPA3	-0.0003076	8443	GTEx	DepMap	Descartes	0.00	1.84
SLC45A3	-0.0003722	9254	GTEx	DepMap	Descartes	0.01	0.43
KIT	-0.0003993	9599	GTEx	DepMap	Descartes	0.03	0.99

B cells: Memory B cells (curated markers)
long-lived mature B lymphocytes which are formed within germinal centers following primary infection and selected for higher-affinity immunoglobulin:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.18e-01
Mean rank of genes in gene set: 11317
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD27	-0.0006226	11317	GTEx	DepMap	Descartes	0	0

T cells: Follicular helper T cells (curated markers)
specialised CD4+ T cell subpopulation found in the periphery within B cell follicles and stimulating the cognate follicular B cells for class-switching:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.24e-01
Mean rank of genes in gene set: 9943.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CXCR5	-0.0003815	9384	GTEx	DepMap	Descartes	0.01	0.69
PDCD1	-0.0004947	10503	GTEx	DepMap	Descartes	0.01	1.94

HSC/MPP: MEMP (curated markers)
shared progenitors which are derived from common myeloid progenitors and will differentiate into megakaryocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.25e-01
Mean rank of genes in gene set: 9971
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ITGA2B	-0.0002626	7776	GTEx	DepMap	Descartes	0.01	0.65
GATA2	-0.0009356	12166	GTEx	DepMap	Descartes	0.08	4.17

T cells: gamma-delta T cells (model markers)
unconventional T lymphocyte subpopulation expressing a gamma-delta T cell receptor complex on the surface to recognise antigens:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.26e-01
Mean rank of genes in gene set: 9304.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.TPM
KLRC2	-0.0003061	8424	GTEx	DepMap	Descartes	0.7
GRIN1	-0.0003915	9508	GTEx	DepMap	Descartes	0.0
KCNK10	-0.0004371	9982	GTEx	DepMap	Descartes	0.0

Macrophages: Macrophages (curated markers)
specialised mononuclear phagocytic cells which recognise:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.28e-01
Mean rank of genes in gene set: 11561
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD68	-0.0006863	11561	GTEx	DepMap	Descartes	0.17	18.78

B cells: Transitional B cells (model markers)
immature B cell precursors in the bone marrow which connect Pre-B cells with mature naive B cells and are subject to the process of B cell selection:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.38e-01
Mean rank of genes in gene set: 11842
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GNG3	-0.0007803	11842	GTEx	DepMap	Descartes	0	0.16

B cells: Follicular B cells (model markers)
resting mature B lymphocytes found in the primary and secondary lymphoid follicles and participating in T cell-dependent immune responses:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.38e-01
Mean rank of genes in gene set: 10223.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
VPREB3	-0.0002807	8048	GTEx	DepMap	Descartes	0.00	0.00
CD83	-0.0011833	12399	GTEx	DepMap	Descartes	0.22	19.72

HSC/MPP: ELP (curated markers)
early lymphoid progenitors which originate from hematopoietic stem cells of the bone marrow and migrate to the thymus:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.44e-01
Mean rank of genes in gene set: 10352
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
RUNX2	-0.0003102	8466	GTEx	DepMap	Descartes	0.16	7.91
LTB	-0.0009912	12238	GTEx	DepMap	Descartes	0.01	2.12

ILC precursor: ILC precursor (curated markers)
innate lymphoid cell precursors which give rise to innate lymphoid cells and lack the characteristics of their mature progenies:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.46e-01
Mean rank of genes in gene set: 9637.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
HPN	-0.0002230	7188	GTEx	DepMap	Descartes	0.00	0.27
LST1	-0.0004942	10497	GTEx	DepMap	Descartes	0.04	4.65
SCN1B	-0.0006073	11228	GTEx	DepMap	Descartes	0.02	1.41

ILC: ILC2 (curated markers)
innate lymphoid cell subpopulation II that promotes type 2 inflammation and is involved in immune response against large extracellular pathogens:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.47e-01
Mean rank of genes in gene set: 9661.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
HPGDS	-0.0002230	7189	GTEx	DepMap	Descartes	0.00	0.00
KLRG1	-0.0003749	9295	GTEx	DepMap	Descartes	0.01	0.45
GATA3	-0.0015147	12500	GTEx	DepMap	Descartes	0.02	0.79

HSC/MPP: Megakaryocyte-erythroid-mast cell progenitor (curated markers)
shared progenitors in the fetal liver which originate from common myeloid progenitors and differentiate into megakaryocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.48e-01
Mean rank of genes in gene set: 12166
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GATA2	-0.0009356	12166	GTEx	DepMap	Descartes	0.08	4.17

T cells: NKT cells (curated markers)
distinct T lymphocyte subpopulation that expresses both alpha/beta T-cell receptors and surface receptors of natural killer cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.49e-01
Mean rank of genes in gene set: 12202
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD8A	-0.0009653	12202	GTEx	DepMap	Descartes	0.01	1.15

ILC: ILC1 (curated markers)
innate lymphoid cell subpopulation I that is non-cytotoxic and has overlapping phenotypes and functions with natural killer cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.52e-01
Mean rank of genes in gene set: 12293
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IKZF3	-0.0010423	12293	GTEx	DepMap	Descartes	0.02	0.36

Erythroid: Mid erythroid (curated markers)
middle erythroid cells in fetal liver:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.53e-01
Mean rank of genes in gene set: 10563.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PRDX2	-0.0004187	9804	GTEx	DepMap	Descartes	0.6	83.56
KCNH2	-0.0006239	11323	GTEx	DepMap	Descartes	0.0	0.11

ILC: Transitional NK (curated markers)
immature natural killer cells which originate from natural killer cell precursors and are committed to mature natural killer cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.55e-01
Mean rank of genes in gene set: 12419
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CCL5	-0.0012415	12419	GTEx	DepMap	Descartes	0.07	8.86

T cells: gamma-delta T cells (curated markers)
unconventional T lymphocyte subpopulation expressing a gamma-delta T cell receptor complex on the surface to recognise antigens:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.55e-01
Mean rank of genes in gene set: 12419
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CCL5	-0.0012415	12419	GTEx	DepMap	Descartes	0.07	8.86

T cells: Tem/Temra cytotoxic T cells (model markers)
terminally differentiated CD8+ cytotoxic T lymphocytes with effector memory phenotypes that re-express CD45RA :
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.56e-01
Mean rank of genes in gene set: 12438
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IL7R	-0.0012827	12438	GTEx	DepMap	Descartes	0.09	4.94

HSC/MPP: Megakaryocyte-erythroid-mast cell progenitor (model markers)
shared progenitors in the fetal liver which originate from common myeloid progenitors and differentiate into megakaryocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.56e-01
Mean rank of genes in gene set: 12454
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CKB	-0.001344	12454	GTEx	DepMap	Descartes	0.31	63.66

T cells: CD8a/b(entry) (curated markers)
T lymphocytes with CD8 alpha/beta heterodimers in the late double-positive (entry) stage during T cell development:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.56e-01
Mean rank of genes in gene set: 12465
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SATB1	-0.0013636	12465	GTEx	DepMap	Descartes	0.11	3.46

DC: DC (model markers)
dendrite-shaped cells which process and present antigens to naïve T cells and induce adaptive immune responses :
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.56e-01
Mean rank of genes in gene set: 12473
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
RGS1	-0.0013967	12473	GTEx	DepMap	Descartes	0.05	4.79

T cells: Tem/Effector helper T cells (model markers)
CD4+ helper T lymphocytes mainly localized in lymphoid and peripheral tissues and presenting an immediate:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.56e-01
Mean rank of genes in gene set: 8805.17
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ANXA1	0.0049648	49	GTEx	DepMap	Descartes	19.94	2433.59
DONSON	-0.0001557	6054	GTEx	DepMap	Descartes	0.17	21.61
AQP3	-0.0005170	10676	GTEx	DepMap	Descartes	0.08	5.55
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60
S100A4	-0.0009428	12175	GTEx	DepMap	Descartes	0.59	231.32
LTB	-0.0009912	12238	GTEx	DepMap	Descartes	0.01	2.12

DC: Migratory DCs (curated markers)
migratory dendritic cells that transport antigens to the draining lymph nodes during both homeostatic conditions and infections:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.57e-01
Mean rank of genes in gene set: 10682
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
EBI3	-0.0003706	9238	GTEx	DepMap	Descartes	0.00	0.41
CCR7	-0.0009077	12126	GTEx	DepMap	Descartes	0.04	5.27

Macrophages: Kidney-resident macrophages (curated markers)
long-lived macrophages resident in the kidney under non-inflammatory conditions and maintaining homeostasis and resolving inflammation :
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.58e-01
Mean rank of genes in gene set: 12515
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD74	-0.0016569	12515	GTEx	DepMap	Descartes	3.79	211.59

B cells: B cells (model markers)
B lymphocytes with diverse cell surface immunoglobulin receptors recognising specific antigenic epitopes and mediating humoral immunity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.62e-01
Mean rank of genes in gene set: 9996.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MALAT1	-0.0002061	6903	GTEx	DepMap	Descartes	100.78	3057.43
CD79A	-0.0005041	10571	GTEx	DepMap	Descartes	0.01	2.20
CD74	-0.0016569	12515	GTEx	DepMap	Descartes	3.79	211.59

T cells: MAIT cells (model markers)
mucosal-associated invariant T cells which have semi-invariant T-cell receptors and are restricted by the MHC I-like molecule MR1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.64e-01
Mean rank of genes in gene set: 9524.75
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
BMP3	-0.0000711	4727	GTEx	DepMap	Descartes	0.00	0.14
KLRG1	-0.0003749	9295	GTEx	DepMap	Descartes	0.01	0.45
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60
IL7R	-0.0012827	12438	GTEx	DepMap	Descartes	0.09	4.94

T cells: CD8a/b(entry) (model markers)
T lymphocytes with CD8 alpha/beta heterodimers in the late double-positive (entry) stage during T cell development:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.65e-01
Mean rank of genes in gene set: 9220
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ITM2A	0.0005227	1671	GTEx	DepMap	Descartes	2.45	436.91
MALAT1	-0.0002061	6903	GTEx	DepMap	Descartes	100.78	3057.43
SATB1	-0.0013636	12465	GTEx	DepMap	Descartes	0.11	3.46
CD44	-0.0017476	12524	GTEx	DepMap	Descartes	2.53	105.47
TMSB10	-0.0019718	12537	GTEx	DepMap	Descartes	7.60	3970.03

ILC: Transitional NK (model markers)
immature natural killer cells which originate from natural killer cell precursors and are committed to mature natural killer cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.67e-01
Mean rank of genes in gene set: 9606
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
AGRP	-0.0000550	4508	GTEx	DepMap	Descartes	0.00	0.00
PRDM16	-0.0004481	10092	GTEx	DepMap	Descartes	0.03	0.69
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60
TYROBP	-0.0009516	12185	GTEx	DepMap	Descartes	0.17	48.35

Monocytes: Classical monocytes (model markers)
CD14+ myeloid mononuclear recirculating leukocytes that are capable of differentiating into macrophages and myeloid lineage dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.70e-01
Mean rank of genes in gene set: 10199.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD207	-0.0001523	5998	GTEx	DepMap	Descartes	0.00	0.00
CST7	-0.0009560	12192	GTEx	DepMap	Descartes	0.04	10.95
EEF1A1	-0.0012138	12409	GTEx	DepMap	Descartes	10.57	574.62

Cycling cells: Cycling DCs (curated markers)
proliferating dendritic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.73e-01
Mean rank of genes in gene set: 11208
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13
TOP2A	-0.0006705	11507	GTEx	DepMap	Descartes	0.03	1.27

Cycling cells: Cycling gamma-delta T cells (curated markers)
proliferating gamma-delta (γδ) T lymphocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.73e-01
Mean rank of genes in gene set: 11208
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13
TOP2A	-0.0006705	11507	GTEx	DepMap	Descartes	0.03	1.27

Cycling cells: Cycling monocytes (curated markers)
proliferating monocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.73e-01
Mean rank of genes in gene set: 11208
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13
TOP2A	-0.0006705	11507	GTEx	DepMap	Descartes	0.03	1.27

Cycling cells: Cycling NK cells (curated markers)
proliferating natural killer cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.73e-01
Mean rank of genes in gene set: 11208
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13
TOP2A	-0.0006705	11507	GTEx	DepMap	Descartes	0.03	1.27

Cycling cells: Cycling T cells (curated markers)
proliferating T lymphocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.73e-01
Mean rank of genes in gene set: 11208
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13
TOP2A	-0.0006705	11507	GTEx	DepMap	Descartes	0.03	1.27

T cells: CD8a/a (curated markers)
unconventional T lymphocytes with CD8 alpha/alpha homodimers binding MHC molecules:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.74e-01
Mean rank of genes in gene set: 11244
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PDCD1	-0.0004947	10503	GTEx	DepMap	Descartes	0.01	1.94
GNG4	-0.0008407	11985	GTEx	DepMap	Descartes	0.03	0.78

Cycling cells: Cycling B cells (curated markers)
proliferating B lymphocytes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.75e-01
Mean rank of genes in gene set: 10363.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD19	-0.0003262	8675	GTEx	DepMap	Descartes	0.00	0.00
MKI67	-0.0005513	10909	GTEx	DepMap	Descartes	0.01	0.13
TOP2A	-0.0006705	11507	GTEx	DepMap	Descartes	0.03	1.27

T cells: Type 1 helper T cells (curated markers)
CD4+ helper T lymphocyte subpopulation which is capable of producing interferon-gamma and modulating cell-mediated immune responses:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.77e-01
Mean rank of genes in gene set: 11375
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
TBX21	-0.0004766	10331	GTEx	DepMap	Descartes	0.00	0.30
CCL5	-0.0012415	12419	GTEx	DepMap	Descartes	0.07	8.86

T cells: Tem/Effector helper T cells PD1+ (curated markers)
CD4+ helper T lymphocyte subpopulation in the thymus which features the expression of programmed cell death protein 1 (PD-1):
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.78e-01
Mean rank of genes in gene set: 11423.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PDCD1	-0.0004947	10503	GTEx	DepMap	Descartes	0.01	1.94
CD4	-0.0010948	12344	GTEx	DepMap	Descartes	0.07	6.46

T cells: T(agonist) (model markers)
unconventional T cell subpopulation in the thymus which expresses MIR155HG and shares some signatures with differentiating regulatory T cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.80e-01
Mean rank of genes in gene set: 9086
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ITM2A	0.0005227	1671	GTEx	DepMap	Descartes	2.45	436.91
COTL1	0.0001072	3162	GTEx	DepMap	Descartes	1.61	81.04
SMS	-0.0003279	8701	GTEx	DepMap	Descartes	0.54	81.24
BIRC3	-0.0014375	12482	GTEx	DepMap	Descartes	0.56	16.51
CD74	-0.0016569	12515	GTEx	DepMap	Descartes	3.79	211.59
PTPRC	-0.0017417	12523	GTEx	DepMap	Descartes	0.09	4.82
B2M	-0.0024801	12548	GTEx	DepMap	Descartes	22.34	2254.76

T cells: Memory CD4+ cytotoxic T cells (curated markers)
CD4+ memory T cells which have cytotoxic activities by secreting granzymes and perforin and by killing target cells on the basis of MHC class II:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.80e-01
Mean rank of genes in gene set: 11546.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IL10	-0.0005270	10749	GTEx	DepMap	Descartes	0.08	6.62
CD4	-0.0010948	12344	GTEx	DepMap	Descartes	0.07	6.46

T cells: T(agonist) (curated markers)
unconventional T cell subpopulation in the thymus which expresses MIR155HG and shares some signatures with differentiating regulatory T cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.82e-01
Mean rank of genes in gene set: 10656.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
SMS	-0.0003279	8701	GTEx	DepMap	Descartes	0.54	81.24
MIR155HG	-0.0005317	10787	GTEx	DepMap	Descartes	0.17	26.19
BIRC3	-0.0014375	12482	GTEx	DepMap	Descartes	0.56	16.51

T cells: Trm cytotoxic T cells (curated markers)
tissue resident CD8+ cytotoxic T lymphocytes mainly localized in epithelial tissues such as gut:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.82e-01
Mean rank of genes in gene set: 11662.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ITGAE	-0.0005456	10875	GTEx	DepMap	Descartes	0.09	4.94
ITGA1	-0.0013259	12450	GTEx	DepMap	Descartes	0.88	15.05

Erythroid: Early erythroid (curated markers)
early erythroid cells which are committed to erythroid lineage cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.84e-01
Mean rank of genes in gene set: 10737
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
APOC1	-0.0003295	8720	GTEx	DepMap	Descartes	0.22	93.75
TESPA1	-0.0006243	11325	GTEx	DepMap	Descartes	0.05	NA
GATA2	-0.0009356	12166	GTEx	DepMap	Descartes	0.08	4.17

HSC/MPP: Early lymphoid/T lymphoid (curated markers)
early lymphoid/T lymphocytes with lymphocyte potential in the fetal liver before T cells emerged from the thymus:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.87e-01
Mean rank of genes in gene set: 11950.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ETS1	-0.0006421	11401	GTEx	DepMap	Descartes	2.32	108.15
GATA3	-0.0015147	12500	GTEx	DepMap	Descartes	0.02	0.79

T cells: Type 17 helper T cells (curated markers)
CD4+ helper T lymphocyte subpopulation which is capable of producing interleukin 17 (IL-17) and mediating protective immunity and autoimmunity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.89e-01
Mean rank of genes in gene set: 12124
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
ZBTB16	-0.0007723	11810	GTEx	DepMap	Descartes	0.14	3.86
IL7R	-0.0012827	12438	GTEx	DepMap	Descartes	0.09	4.94

B cells: Memory B cells (model markers)
long-lived mature B lymphocytes which are formed within germinal centers following primary infection and selected for higher-affinity immunoglobulin:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.90e-01
Mean rank of genes in gene set: 12197.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
HES1	-0.0007954	11880	GTEx	DepMap	Descartes	2.88	519.69
CD74	-0.0016569	12515	GTEx	DepMap	Descartes	3.79	211.59

T cells: Tem/Trm cytotoxic T cells (curated markers)
CD8+ cytotoxic T lymphocytes mainly localized in lymphoid and peripheral tissues and presenting an immediate:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.91e-01
Mean rank of genes in gene set: 12310.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD8A	-0.0009653	12202	GTEx	DepMap	Descartes	0.01	1.15
CCL5	-0.0012415	12419	GTEx	DepMap	Descartes	0.07	8.86

T cells: Tcm/Naive cytotoxic T cells (model markers)
CD8+ cytotoxic T lymphocytes mainly localized in secondary lymphoid tissues and sustaining the responses by proliferating and producing new effectors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.91e-01
Mean rank of genes in gene set: 12375
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD8A	-0.0009653	12202	GTEx	DepMap	Descartes	0.01	1.15
B2M	-0.0024801	12548	GTEx	DepMap	Descartes	22.34	2254.76

T cells: Treg(diff) (curated markers)
unconventional T lymphocyte subpopulation in the thymus which connects αβ T cells and canonical regulatory T cells and is still differentiating:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.92e-01
Mean rank of genes in gene set: 11280
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
IKZF4	-0.0004828	10397	GTEx	DepMap	Descartes	0.14	7.84
CD27	-0.0006226	11317	GTEx	DepMap	Descartes	0.00	0.00
CCR7	-0.0009077	12126	GTEx	DepMap	Descartes	0.04	5.27

T cells: NKT cells (model markers)
distinct T lymphocyte subpopulation that expresses both alpha/beta T-cell receptors and surface receptors of natural killer cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.94e-01
Mean rank of genes in gene set: 11589.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
PRDM16	-0.0004481	10092	GTEx	DepMap	Descartes	0.03	0.69
ITM2C	-0.0009996	12241	GTEx	DepMap	Descartes	0.48	51.84
CD52	-0.0012774	12436	GTEx	DepMap	Descartes	0.01	4.64

ILC: ILC (model markers)
specialised innate immune cells from the lymphoid lineage but without antigen-specific T-cell receptors on the surface:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.95e-01
Mean rank of genes in gene set: 10929.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GRIN1	-0.0003915	9508	GTEx	DepMap	Descartes	0.00	0.00
AREG	-0.0004532	10133	GTEx	DepMap	Descartes	0.09	15.23
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60
IL7R	-0.0012827	12438	GTEx	DepMap	Descartes	0.09	4.94

T cells: CD8a/a (model markers)
unconventional T lymphocytes with CD8 alpha/alpha homodimers binding MHC molecules:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.95e-01
Mean rank of genes in gene set: 9811.71
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
NUCB2	0.0001068	3168	GTEx	DepMap	Descartes	0.63	47.90
MALAT1	-0.0002061	6903	GTEx	DepMap	Descartes	100.78	3057.43
LEF1	-0.0005289	10761	GTEx	DepMap	Descartes	0.37	25.29
CD27	-0.0006226	11317	GTEx	DepMap	Descartes	0.00	0.00
CTSW	-0.0008107	11920	GTEx	DepMap	Descartes	0.00	0.30
CD8A	-0.0009653	12202	GTEx	DepMap	Descartes	0.01	1.15
PRKCH	-0.0012156	12411	GTEx	DepMap	Descartes	0.10	4.09

ILC: CD16- NK cells (model markers)
CD16- granular lymphocytes that play protective roles against both infectious pathogens and cancer using antibody-dependent cell-mediated cytotoxicity:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.96e-01
Mean rank of genes in gene set: 9886.43
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
GSTP1	0.0001775	2822	GTEx	DepMap	Descartes	1.55	217.78
LDB2	-0.0003561	9051	GTEx	DepMap	Descartes	0.35	36.85
CLIC3	-0.0004084	9691	GTEx	DepMap	Descartes	0.00	0.43
KLRB1	-0.0007118	11639	GTEx	DepMap	Descartes	0.01	0.60
FCER1G	-0.0007977	11889	GTEx	DepMap	Descartes	0.08	20.36
CXCL3	-0.0008145	11928	GTEx	DepMap	Descartes	2.07	219.41
TYROBP	-0.0009516	12185	GTEx	DepMap	Descartes	0.17	48.35

T cells: Tcm/Naive cytotoxic T cells (curated markers)
CD8+ cytotoxic T lymphocytes mainly localized in secondary lymphoid tissues and sustaining the responses by proliferating and producing new effectors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-01
Mean rank of genes in gene set: 12195.33
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CCR7	-0.0009077	12126	GTEx	DepMap	Descartes	0.04	5.27
CD8A	-0.0009653	12202	GTEx	DepMap	Descartes	0.01	1.15
SELL	-0.0010171	12258	GTEx	DepMap	Descartes	0.14	18.18

T cells: Tcm/Naive helper T cells (curated markers)
CD4+ helper T lymphocytes mainly localized in secondary lymphoid tissues and sustaining the responses by proliferating and producing new effectors:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-01
Mean rank of genes in gene set: 12242.67
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CCR7	-0.0009077	12126	GTEx	DepMap	Descartes	0.04	5.27
SELL	-0.0010171	12258	GTEx	DepMap	Descartes	0.14	18.18
CD4	-0.0010948	12344	GTEx	DepMap	Descartes	0.07	6.46

T cells: Tem/Trm cytotoxic T cells (model markers)
CD8+ cytotoxic T lymphocytes mainly localized in lymphoid and peripheral tissues and presenting an immediate:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-01
Mean rank of genes in gene set: 12271
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CST7	-0.0009560	12192	GTEx	DepMap	Descartes	0.04	10.95
CD8A	-0.0009653	12202	GTEx	DepMap	Descartes	0.01	1.15
CCL5	-0.0012415	12419	GTEx	DepMap	Descartes	0.07	8.86

Double-negative thymocytes: Double-negative thymocytes (model markers)
the early immature thymocytes from the thymus in the double negative (co-receptors CD4- and CD8-) stage:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-01
Mean rank of genes in gene set: 10535.5
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
FXYD2	-0.0001321	5676	GTEx	DepMap	Descartes	0.00	0.17
MZB1	-0.0002841	8103	GTEx	DepMap	Descartes	0.00	NA
SELL	-0.0010171	12258	GTEx	DepMap	Descartes	0.14	18.18
PDLIM1	-0.0011047	12358	GTEx	DepMap	Descartes	1.02	135.48
CD7	-0.0011245	12371	GTEx	DepMap	Descartes	0.07	8.24
MFAP4	-0.0013182	12447	GTEx	DepMap	Descartes	1.52	164.83

T cells: Trm cytotoxic T cells (model markers)
tissue resident CD8+ cytotoxic T lymphocytes mainly localized in epithelial tissues such as gut:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-01
Mean rank of genes in gene set: 12353
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
CD8A	-0.0009653	12202	GTEx	DepMap	Descartes	0.01	1.15
CCL5	-0.0012415	12419	GTEx	DepMap	Descartes	0.07	8.86
IL7R	-0.0012827	12438	GTEx	DepMap	Descartes	0.09	4.94

T cells: Regulatory T cells (model markers)
T cell subpopulation which modulates immune responses and regulates other cells through direct cell-cell contact and cytokine release:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-01
Mean rank of genes in gene set: 11603.25
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
RTKN2	-0.0004448	10059	GTEx	DepMap	Descartes	0.04	1.24
TIGIT	-0.0006260	11337	GTEx	DepMap	Descartes	0.00	0.25
RGS1	-0.0013967	12473	GTEx	DepMap	Descartes	0.05	4.79
SRGN	-0.0020768	12544	GTEx	DepMap	Descartes	2.32	287.99

T cells: Treg(diff) (model markers)
unconventional T lymphocyte subpopulation in the thymus which connects αβ T cells and canonical regulatory T cells and is still differentiating:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.00e+00
Mean rank of genes in gene set: 12410
Rank on gene expression program of genes in gene set:

Genes	Weight	Rank	GTEx	DepMap	Decartes	Mean.Counts	Mean.TPM
S100A4	-0.0009428	12175	GTEx	DepMap	Descartes	0.59	231.32
TSC22D3	-0.0013898	12469	GTEx	DepMap	Descartes	0.97	92.16
RGS1	-0.0013967	12473	GTEx	DepMap	Descartes	0.05	4.79
PTPRC	-0.0017417	12523	GTEx	DepMap	Descartes	0.09	4.82

B cells: Naive B cells (curated markers)
mature B lymphocytes which express cell-surface IgM and IgD and have not been exposed to/activated by antigens:
DC: DC (curated markers)
dendrite-shaped cells which process and present antigens to naïve T cells and induce adaptive immune responses :
DC: DC2 (curated markers)
conventional type 2 dendritic cells which constitute the major subset of myeloid dendritic cells:
DC: DC3 (curated markers)
a dendritic cell subtype found in bone marrow which shares features with monocytes and conventional type 2 dendritic cells:
DC precursor: DC precursor (curated markers)
dendritic cell precursors that differentiate into dendritic cells:
Erythrocytes: Erythrocytes (curated markers)
biconcave enucleated red blood cells filled with hemoglobin to transport oxygen and carbon dioxide between the lungs and tissues:
Erythroid: Late erythroid (curated markers)
late erythroid cells following early and middle erythroid cells:
HSC/MPP: CMP (curated markers)
highly proliferative common myeloid progenitors which later give rise to granulocyte-monocyte progenitors and megakaryocyte-erythroid progenitors:
HSC/MPP: GMP (curated markers)
hematopoietic granulocyte-monocyte progenitors that are committed to the granulocyte and monocyte lineage cells:
HSC/MPP: Neutrophil-myeloid progenitor (curated markers)
progenitors of neutrophils and myeloid cells which are transitioned from hematopoietic stem cells and multipotent progenitors:
ILC: CD16+ NK cells (curated markers)
CD16+ granular lymphocytes that play protective roles against both infectious pathogens and cancer using antibody-dependent cell-mediated cytotoxicity:
ILC: NK cells (curated markers)
granular lymphocytes that play protective roles against both infectious pathogens and cancer using antibody-dependent cell-mediated cytotoxicity:
Monocytes: Non-classical monocytes (curated markers)
CD16+ myeloid mononuclear recirculating leukocytes that are capable of differentiating into macrophages and myeloid lineage dendritic cells:
Monocytes: Monocytes (curated markers)
myeloid mononuclear recirculating leukocytes that are capable of differentiating into macrophages and myeloid lineage dendritic cells:
Myelocytes: Myelocytes (curated markers)
early granulocyte precursors that are derive from promyelocytes and later mature into metamyelocytes:
Promyelocytes: Promyelocytes (curated markers)
early granulocyte progenitors in the bone marrow which are derived from the myeloblasts and develop into the myelocytes:
T cells: Tem/Temra cytotoxic T cells (curated markers)
terminally differentiated CD8+ cytotoxic T lymphocytes with effector memory phenotypes that re-express CD45RA :
B-cell lineage: Pre-pro-B cells (model markers)
the earliest primitive B cell progenitors which express CD34 and SPINK2:
B-cell lineage: Pro-B cells (model markers)
early B lymphocyte progenitors undergoing D-J joining on the H chain chromosome and joining of a V segment to the rearranged D-J:
Cycling cells: Cycling gamma-delta T cells (model markers)
proliferating gamma-delta (γδ) T lymphocytes:
Cycling cells: Cycling monocytes (model markers)
proliferating monocytes:
DC: Transitional DC (model markers)
immature dendritic cells which follow dendritic cell precursors and are committed to the mature dendritic cells:
Erythroid: Mid erythroid (model markers)
middle erythroid cells in fetal liver:
Granulocytes: Neutrophils (model markers)
the most abundant type of granulocytes that contains distinctive cytoplasmic granules and forms an essential part of the innate immune system:
HSC/MPP: ELP (model markers)
early lymphoid progenitors which originate from hematopoietic stem cells of the bone marrow and migrate to the thymus:
HSC/MPP: GMP (model markers)
hematopoietic granulocyte-monocyte progenitors that are committed to the granulocyte and monocyte lineage cells:
HSC/MPP: MEMP (model markers)
shared progenitors which are derived from common myeloid progenitors and will differentiate into megakaryocytes:
ILC: ILC1 (model markers)
innate lymphoid cell subpopulation I that is non-cytotoxic and has overlapping phenotypes and functions with natural killer cells:
Macrophages: Kidney-resident macrophages (model markers)
long-lived macrophages resident in the kidney under non-inflammatory conditions and maintaining homeostasis and resolving inflammation :
Megakaryocytes/platelets: Megakaryocytes/platelets (model markers)
large multinucleated cells with numerous azurophilic granules and with mature blood platelets released from the cytoplasm:
MNP: MNP (model markers)
mononuclear phagocytes including dendritic cells: