Program: 5. Neuroblastoma: Adrenergic I (pre-chromaffin).

Symphathoblasts Fig2 (Kamenva)
Marker genes were obtained from Fig. 2H of Kameneva et al (PMID 33833454) for symphathoblasts (differentiating from SCPs):
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.84e-04
Mean rank of genes in gene set: 506
Rank on gene expression program of genes in gene set:

Late Neuroblasts (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types. Note: the Jansky Late Neuroblast signature is the same as the earlier Neuroblast signature, but NEFM and ALK are now absent (meaning lower expressed at later stages of differentiation):
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.85e-04
Mean rank of genes in gene set: 507.6
Rank on gene expression program of genes in gene set:

Committed Progenitor (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Committed Progenitor subcluster (seems to mean progenitor of chromaffin cells), which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.43e-04
Mean rank of genes in gene set: 3014.13
Rank on gene expression program of genes in gene set:

Chromaffin and connecting progenitor cells (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.74e-04
Mean rank of genes in gene set: 15.5
Rank on gene expression program of genes in gene set:

Late chromaffin cells (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types. Note: this late chromaffin cell signature also includes PNMT, which is an enzyme catalyzing methylation of norepinephrine to form epinephrine; this marker is absent from the ealier less differentiated chromaffin cells (which may have been referred to as sympathoblasts in other datasets, I think):
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.74e-04
Mean rank of genes in gene set: 15.5
Rank on gene expression program of genes in gene set:

Neuroblast (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Neuroblast subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.45e-04
Mean rank of genes in gene set: 2570.18
Rank on gene expression program of genes in gene set:

Symphathoblasts (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. Several of these markers are also (more weakly) expressed in chromaffin cells. The authors data suggest Symphthoblasts give rise to chromaffin cells.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.65e-04
Mean rank of genes in gene set: 157.25
Rank on gene expression program of genes in gene set:

E Chromaffin (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Epinepherine Chromaffin subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.82e-04
Mean rank of genes in gene set: 1472.67
Rank on gene expression program of genes in gene set:

Bridge region adrenergic transition (Olsen)
As above but for cells in the mesenchymal transitioning to adrenergic region:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.37e-03
Mean rank of genes in gene set: 12.33
Rank on gene expression program of genes in gene set:

Meta_7 undefined sympathetic (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.39e-03
Mean rank of genes in gene set: 20.33
Rank on gene expression program of genes in gene set:

Sympathobasts or chromaffin (Kildisiute)
Sympathoblast and chromaffin markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.49e-03
Mean rank of genes in gene set: 62.67
Rank on gene expression program of genes in gene set:

Chromaffin Cells (Dong)
Obtained from Fig. 3c of Dong et al. (PMID 32946775). Jansky et al (PMID) criticize this choice of markers, stating: the neuronal markers NPY, PRPH, NTRK1 and ISL1 are used to annotate chromaffin cells; these were clearly expressed in neuroblasts in our analyses.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.56e-03
Mean rank of genes in gene set: 94.33
Rank on gene expression program of genes in gene set:

Bridge (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Bridge subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.84e-03
Mean rank of genes in gene set: 3488.33
Rank on gene expression program of genes in gene set:

Pre-E Chromafin (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Pre Epinepherine Chromaffin subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.84e-03
Mean rank of genes in gene set: 3488.5
Rank on gene expression program of genes in gene set:

Adrenergic (Olsen)
Markers listed in on page 3 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are for adrenergic genes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.20e-03
Mean rank of genes in gene set: 7
Rank on gene expression program of genes in gene set:

Chromaffin (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. These markers are expressed at a lower level in symphathoblasts.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.23e-03
Mean rank of genes in gene set: 11
Rank on gene expression program of genes in gene set:

Adrenergic mature (olsen)
Stated on pages 6 and 7 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - it is argued that upon reanalysis of the adrenergic neuroblastoma cells, there is further heterogeneity, grouped into proliferating, mature and immature cell populations. These markers are specific to the mature adrenergic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.29e-03
Mean rank of genes in gene set: 18.5
Rank on gene expression program of genes in gene set:

Meta5 unclear (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.93e-03
Mean rank of genes in gene set: 96
Rank on gene expression program of genes in gene set:

Definitive Zone (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Definitive Zone subcluster, which is part of the Cortex cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.40e-03
Mean rank of genes in gene set: 1944.5
Rank on gene expression program of genes in gene set:

Neuroblastoma (Kildisiute)
Neuroblastoma markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.80e-03
Mean rank of genes in gene set: 194
Rank on gene expression program of genes in gene set:

Chromaffin cell development (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.04e-03
Mean rank of genes in gene set: 220
Rank on gene expression program of genes in gene set:

Chromaffin cell-like (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 9.98e-03
Mean rank of genes in gene set: 314
Rank on gene expression program of genes in gene set:

Protein maturation (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.65e-02
Mean rank of genes in gene set: 1814.33
Rank on gene expression program of genes in gene set:

Neuroblastoma SCPs but not normal SCPs (Olsen)
The same as above:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.41e-02
Mean rank of genes in gene set: 1215.5
Rank on gene expression program of genes in gene set:

Sympathoblasts (Kildisiute)
Sympathoblasts markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.85e-02
Mean rank of genes in gene set: 2294.67
Rank on gene expression program of genes in gene set:

Meta_9 ATRX (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.95e-02
Mean rank of genes in gene set: 1439
Rank on gene expression program of genes in gene set:

Neuroblasts (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.19e-02
Mean rank of genes in gene set: 3738.14
Rank on gene expression program of genes in gene set:

Symphathoadrenal cells (Yuan)
Obtained from Fig. 1 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.20e-02
Mean rank of genes in gene set: 16
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: SOX10

Adrenergic neuroblatoma (Olsen)
Stated on page 6 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - neuroblastoma adrenergic markers derived from their data, which are state as resembling sympathoblasts and lacking chromffin markers such as PNMT:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.25e-02
Mean rank of genes in gene set: 35
Rank on gene expression program of genes in gene set:

Chromaffin cells Fig2 (Kamenva)
Marker genes were obtained from Fig. 2H of Kameneva et al (PMID 33833454) for chromaffin cells (differentiating from symphathoblasts):
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.60e-02
Mean rank of genes in gene set: 2950.33
Rank on gene expression program of genes in gene set:

Bridge region SCP-adrenergic transition (Olsen)
As above but for cells in the mesenchymal transitioning to SCP-adrenergic region:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.01e-01
Mean rank of genes in gene set: 3967.25
Rank on gene expression program of genes in gene set:

Adrenergic immature (Olsen)
Stated on pages 6 and 7 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - it is argued that upon reanalysis of the adrenergic neuroblastoma cells, there is further heterogeneity, grouped into proliferating, mature and immature cell populations. These is a marker listed for the immature adrenergic cells. SOX11 is referenced as important in early phases of pro-adrenergic differentiation (cites PMID 20147379) whereas SOX4 appears later. SOX11 is also associated with poor outcome in neuroblatoma bulk RNA-seq data (Olsen analysis).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.81e-01
Mean rank of genes in gene set: 2978
Rank on gene expression program of genes in gene set:

Melanocytes (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.02e-01
Mean rank of genes in gene set: 4532.33
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: TYR

Muscle progenitor (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that PAX7+ Muscle progenitor were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.29e-01
Mean rank of genes in gene set: 4370.5
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: PAX3

Pigmentation (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, Skin pigmentation genes, identified in melanoma.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.31e-01
Mean rank of genes in gene set: 4940.5
Rank on gene expression program of genes in gene set:

Liver Primordium (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.34e-01
Mean rank of genes in gene set: 4413.5
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: ALDOB HNF4A

Angioblast (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Angioblast subcluster of Endothelium cluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.45e-01
Mean rank of genes in gene set: 4833.33
Rank on gene expression program of genes in gene set:

Erythrocytes (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that HBA2+ Erythrocytes were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.48e-01
Mean rank of genes in gene set: 3807
Rank on gene expression program of genes in gene set:

Nerves and neurofiliment (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - NF200 is expressed in neurofiliments and is found close to SCPs in normal tissue, NRG1 is secreted by neurons, to which ERBB3 (on glia) binds.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.70e-01
Mean rank of genes in gene set: 4057
Rank on gene expression program of genes in gene set:

Adrenal Premordium (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Adrenal Premordium subcluster, which is part of the Cortex cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.85e-01
Mean rank of genes in gene set: 5745.25
Rank on gene expression program of genes in gene set:

Stress response (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.61e-01
Mean rank of genes in gene set: 6071
Rank on gene expression program of genes in gene set:

EMT III (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this EMT program was enriched in non-cycling cells.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.94e-01
Mean rank of genes in gene set: 6246
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: COL17A1

Myelination (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are state as being involved in Myelination and are expressed in the Schwann cell precursor cluster of their UMAP (Fig. 1D), these are suggested to represent more mature myelinating Schwann cells. MPZ is Myelin Protien Zero.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.48e-01
Mean rank of genes in gene set: 6583
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: PMP2

Proteasomal degradation (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this is for proteasomal degradataion:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.48e-01
Mean rank of genes in gene set: 6472
Rank on gene expression program of genes in gene set:

Schwann cell precursor (Kildisiute)
SCP markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.22e-01
Mean rank of genes in gene set: 6926
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: SOX10

Schwann cell precursors (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.22e-01
Mean rank of genes in gene set: 6926
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: CDH19 SOX10

Meta_8 undefined undifferentiated neuronal (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.36e-01
Mean rank of genes in gene set: 7164.5
Rank on gene expression program of genes in gene set:

Erythroid cells (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.00e-01
Mean rank of genes in gene set: 8178
Rank on gene expression program of genes in gene set:

SCPs mouse unique (Olsen)
Selected list of SCP marker genes shown in Fig. 2B of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these genes were unique to mouse SCPs as determined by the linearge tracing study Furlan et al. (Science 2017, PMID 28684471).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.04e-01
Mean rank of genes in gene set: 7246.25
Rank on gene expression program of genes in gene set:

Stress response (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.16e-01
Mean rank of genes in gene set: 7201.4
Rank on gene expression program of genes in gene set:

EpiSen (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, an epithelial senescence associated program.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.23e-01
Mean rank of genes in gene set: 7349
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: SPRR1B

Mesenchymal (Kildisiute)
Mesenchymal markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.25e-01
Mean rank of genes in gene set: 8444
Rank on gene expression program of genes in gene set:

Stromal 1 (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Stroma subcluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.27e-01
Mean rank of genes in gene set: 7101.57
Rank on gene expression program of genes in gene set:

Stromal 3 (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Stroma subcluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.40e-01
Mean rank of genes in gene set: 7619.33
Rank on gene expression program of genes in gene set:

Myocytes (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that MYH3+ Myocytes were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.43e-01
Mean rank of genes in gene set: 7945
Rank on gene expression program of genes in gene set:

No detectable expression in this dataset: MYH8

EMT I melanoma (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this EMT program was melanoma specific.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.45e-01
Mean rank of genes in gene set: 7654
Rank on gene expression program of genes in gene set:

Hepatocytes (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that ALB+ Hepatocytes were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D. The main text also speculated that these hepatocytes were probably derived from neighboring tissue, and hence artifactual, but there is no evidence presented for this.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.49e-01
Mean rank of genes in gene set: 7991.5
Rank on gene expression program of genes in gene set:

SCPs mouse and neuroblastoma (Olsen)
Similar to above, but for genes common to neuroblastoma SCP-like cells (as determined from their UMAP plot) and mouse SCP cells.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.55e-01
Mean rank of genes in gene set: 7030.36
Rank on gene expression program of genes in gene set: