Program: 16. M-MDSC.

Melanocytes (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.97e-02
Mean rank of genes in gene set: 1968.67
Rank on gene expression program of genes in gene set:

Pigmentation (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, Skin pigmentation genes, identified in melanoma.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.51e-02
Mean rank of genes in gene set: 2727
Rank on gene expression program of genes in gene set:

PMN-MDSC
These marker genes were curated for MDSC subtypes as reviewed in Veglia et. al. (PMID 33526920):
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.18e-02
Mean rank of genes in gene set: 4596.62
Rank on gene expression program of genes in gene set:

Bridge (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.14e-02
Mean rank of genes in gene set: 2862
Rank on gene expression program of genes in gene set:

EpiSen (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, an epithelial senescence associated program.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.04e-02
Mean rank of genes in gene set: 3735
Rank on gene expression program of genes in gene set:

Fetal Zone (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Fetal Zone subcluster, which is part of the Cortex cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.65e-02
Mean rank of genes in gene set: 1338
Rank on gene expression program of genes in gene set:

Muscle progenitor (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that PAX7+ Muscle progenitor were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.10e-01
Mean rank of genes in gene set: 3135
Rank on gene expression program of genes in gene set:

HSCs and immune cells (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.24e-01
Mean rank of genes in gene set: 3861.33
Rank on gene expression program of genes in gene set:

Proliferating Cells
These are canonical marker genes of cycling cells, obtained from Fig. 3 of Hsiao et al. (PMID 32312741):
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.24e-01
Mean rank of genes in gene set: 4185
Rank on gene expression program of genes in gene set:

Adrenergic proliferating cluster (Olsen)
Stated on pages 6 and 7 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - it is argued that upon reanalysis of the adrenergic neuroblastoma cells, there is further heterogeneity, grouped into proliferating, mature and immature cell populations. These genes were found as markers for proliferating adrenergic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.33e-01
Mean rank of genes in gene set: 3422
Rank on gene expression program of genes in gene set:

Liver Primordium (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.46e-01
Mean rank of genes in gene set: 3580.5
Rank on gene expression program of genes in gene set:

Erythroid cells (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.64e-01
Mean rank of genes in gene set: 2731
Rank on gene expression program of genes in gene set:

IFN Response (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this program contained interferon response genes.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.66e-01
Mean rank of genes in gene set: 4701.2
Rank on gene expression program of genes in gene set:

Adrenal cortex (Kildisiute)
Adrenal cortex markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.70e-01
Mean rank of genes in gene set: 2813
Rank on gene expression program of genes in gene set:

Adrenal gland cortex (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. :
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.70e-01
Mean rank of genes in gene set: 2813
Rank on gene expression program of genes in gene set:

Protein maturation (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 1.82e-01
Mean rank of genes in gene set: 4376.67
Rank on gene expression program of genes in gene set:

Stress response (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.14e-01
Mean rank of genes in gene set: 4993.2
Rank on gene expression program of genes in gene set:

Endothelium (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.57e-01
Mean rank of genes in gene set: 5091.5
Rank on gene expression program of genes in gene set:

Endothelial cells (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that PTPRB+ Endothelial cells were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.95e-01
Mean rank of genes in gene set: 5146
Rank on gene expression program of genes in gene set:

Neuroblastoma SCPs but not normal SCPs (Olsen)
The same as above:
Wilcoxon ranksum test P-value for gene set overrepresentation: 2.98e-01
Mean rank of genes in gene set: 4915.5
Rank on gene expression program of genes in gene set:

Antigen Presenting CAF
These marker genes were curated across cancer subtypes in multiple organ systems as reviewed in Lavie et. al. (PMID 35883004) and contain antigen presenting specific CAF genes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.51e-01
Mean rank of genes in gene set: 5875.42
Rank on gene expression program of genes in gene set:

Endothelial Cells (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.70e-01
Mean rank of genes in gene set: 5427.5
Rank on gene expression program of genes in gene set:

Kidney Primordium (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. :
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.84e-01
Mean rank of genes in gene set: 5203
Rank on gene expression program of genes in gene set:

Meta_8 undefined undifferentiated neuronal (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 3.96e-01
Mean rank of genes in gene set: 5596.5
Rank on gene expression program of genes in gene set:

Definitive Zone (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Definitive Zone subcluster, which is part of the Cortex cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.14e-01
Mean rank of genes in gene set: 5880
Rank on gene expression program of genes in gene set:

Noninflammatory Macrophages (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.14e-01
Mean rank of genes in gene set: 5718.5
Rank on gene expression program of genes in gene set:

Bridge (Kildisiute)
Bridge cell (cites Furlan 2017) markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.47e-01
Mean rank of genes in gene set: 5796
Rank on gene expression program of genes in gene set:

Chromaffin cell-like (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.93e-01
Mean rank of genes in gene set: 6227.5
Rank on gene expression program of genes in gene set:

Angioblast (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Angioblast subcluster of Endothelium cluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 4.95e-01
Mean rank of genes in gene set: 6248
Rank on gene expression program of genes in gene set:

Immune (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the broad Immune cluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.02e-01
Mean rank of genes in gene set: 6288
Rank on gene expression program of genes in gene set:

Myelination (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are state as being involved in Myelination and are expressed in the Schwann cell precursor cluster of their UMAP (Fig. 1D), these are suggested to represent more mature myelinating Schwann cells. MPZ is Myelin Protien Zero.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.17e-01
Mean rank of genes in gene set: 6381.5
Rank on gene expression program of genes in gene set:

EMT III (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this EMT program was enriched in non-cycling cells.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.19e-01
Mean rank of genes in gene set: 6360.75
Rank on gene expression program of genes in gene set:

Stromal 1 (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for Stroma subcluster:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.66e-01
Mean rank of genes in gene set: 6503.57
Rank on gene expression program of genes in gene set:

Normal Schwann cell precurors, but not Neuroblastoma Scps (Olsen)
Based on their neuroblastoma data and stated on page 6 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.67e-01
Mean rank of genes in gene set: 6882
Rank on gene expression program of genes in gene set:

Nerves and neurofiliment (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - NF200 is expressed in neurofiliments and is found close to SCPs in normal tissue, NRG1 is secreted by neurons, to which ERBB3 (on glia) binds.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.67e-01
Mean rank of genes in gene set: 6887
Rank on gene expression program of genes in gene set:

Sympathoblasts (Kildisiute)
Sympathoblasts markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.71e-01
Mean rank of genes in gene set: 6646.67
Rank on gene expression program of genes in gene set:

Hepatocytes (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that ALB+ Hepatocytes were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D. The main text also speculated that these hepatocytes were probably derived from neighboring tissue, and hence artifactual, but there is no evidence presented for this.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.78e-01
Mean rank of genes in gene set: 6777
Rank on gene expression program of genes in gene set:

Schwann cell precursor Fig2 (Kamenva)
Marker genes were obtained from Fig. 2H of Kameneva et al (PMID 33833454). These genes were used to show cells transitioning from a SCP to a symphathoblast state in their human fetal single-cell RNA-seq data.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 5.81e-01
Mean rank of genes in gene set: 6509.5
Rank on gene expression program of genes in gene set:

Erythrocytes (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that HBA2+ Erythrocytes were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.23e-01
Mean rank of genes in gene set: 7411
Rank on gene expression program of genes in gene set:

Proteasomal degradation (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this is for proteasomal degradataion:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.35e-01
Mean rank of genes in gene set: 6833.8
Rank on gene expression program of genes in gene set:

Bridge region SCP-like (Olsen)
As above but for the SCP-like region:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.61e-01
Mean rank of genes in gene set: 7026
Rank on gene expression program of genes in gene set:

Axon guidance (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are for axon guidance instead of myelination:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.75e-01
Mean rank of genes in gene set: 7922
Rank on gene expression program of genes in gene set:

Adrenergic mature (olsen)
Stated on pages 6 and 7 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - it is argued that upon reanalysis of the adrenergic neuroblastoma cells, there is further heterogeneity, grouped into proliferating, mature and immature cell populations. These markers are specific to the mature adrenergic cells:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.84e-01
Mean rank of genes in gene set: 7498
Rank on gene expression program of genes in gene set:

Other T cells (Yuan)
Obtained from Fig. 6 of Yuan et al. https://www.biorxiv.org/content/10.1101/2020.05.15.097469v1:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.85e-01
Mean rank of genes in gene set: 6986
Rank on gene expression program of genes in gene set:

Myocytes (Jansky)
Mentioned in the main text (Jansky et al, Nature Genetics (2021)), page 1, that MYH3+ Myocytes were identified in their fetal adrenal glands, this is also highlighted in the UMAP plot on their Fig 1B. Additional genes also shown in their Extended data Figure 2D.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.86e-01
Mean rank of genes in gene set: 7513
Rank on gene expression program of genes in gene set:

SCPs mouse unique (Olsen)
Selected list of SCP marker genes shown in Fig. 2B of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these genes were unique to mouse SCPs as determined by the linearge tracing study Furlan et al. (Science 2017, PMID 28684471).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 6.98e-01
Mean rank of genes in gene set: 7216
Rank on gene expression program of genes in gene set:

Schwann cell precursor (Kildisiute)
SCP markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.01e-01
Mean rank of genes in gene set: 7379.33
Rank on gene expression program of genes in gene set:

Schwann cell precursors (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.01e-01
Mean rank of genes in gene set: 7379.33
Rank on gene expression program of genes in gene set:

Meta_9 ATRX (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.01e-01
Mean rank of genes in gene set: 7628
Rank on gene expression program of genes in gene set:

Chromaffin cells Fig2 (Kamenva)
Marker genes were obtained from Fig. 2H of Kameneva et al (PMID 33833454) for chromaffin cells (differentiating from symphathoblasts):
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.12e-01
Mean rank of genes in gene set: 7443
Rank on gene expression program of genes in gene set:

Pre-E Chromafin (Hanemaaijer)
Marker genes obtained from Supplementary Table SD of Hanemaaijer et al (PMID 33500353). The authors generated single-cell RNA-seq data (sort-seq, 2,229 cells total) from mouse adrenal glads at E13.5, E14.5, E17.5, E18.5, P1 and P5. These were marker genes that matched with a similar dataset generated by Furlan et al (PMID 28684471). This particular set of markers are for the Pre Epinepherine Chromaffin subcluster, which is part of the Adrenal Medulla cluster.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.21e-01
Mean rank of genes in gene set: 6887.42
Rank on gene expression program of genes in gene set:

Cell cycle (Dong)
Markers obtained in computationally inferred meta program (by NMF applied to their neuroblastoma sc-RNA-seq data) from Fig. 5(a) of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.27e-01
Mean rank of genes in gene set: 7535.33
Rank on gene expression program of genes in gene set:

Neuroblastoma (Kildisiute)
Neuroblastoma markers obtained from Kildisiute et al, Supplmenentary Table 2, references supporting these genes are provided in Supp Table S2 of Kildisiute et al (PMID 33547074) https://www.science.org/doi/suppl/10.1126/sciadv.abd3311/suppl_file/abd3311_tables_s1_to_s12.xlsx:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.82e-01
Mean rank of genes in gene set: 8267
Rank on gene expression program of genes in gene set:

Schwann cell precursor (Olsen)
Markers listed in on page 4 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are stated as being characteristic of Schwann cell lineage and Furlan et al. (Science 2017, PMID 28684471) is referenced to support this. These cell types seem to be claimed to form a bridge between the mesenchymal and adrenergic cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.83e-01
Mean rank of genes in gene set: 7909.67
Rank on gene expression program of genes in gene set:

Schwann cell precursor-like Fig 1D (Olsen)
Similar to above, but for selected SCP marker genes.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.83e-01
Mean rank of genes in gene set: 7909.67
Rank on gene expression program of genes in gene set:

Chromaffin Cells (Dong)
Obtained from Fig. 3c of Dong et al. (PMID 32946775). Jansky et al (PMID) criticize this choice of markers, stating: the neuronal markers NPY, PRPH, NTRK1 and ISL1 are used to annotate chromaffin cells; these were clearly expressed in neuroblasts in our analyses.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 7.86e-01
Mean rank of genes in gene set: 7932
Rank on gene expression program of genes in gene set:

Neuroblasts (Jansky Fig1E)
Heatmap in Fig. 1E, marker genes derived from Jansks scRNA-seq data for adrenal medulla cell types.:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.04e-01
Mean rank of genes in gene set: 7445.71
Rank on gene expression program of genes in gene set:

EMT II (Kinker)
These marker genes were obtained in a pan cancer cell lines NMF analysis of scRNA-eq data in Kinker et al (PMID 33128048) - 10 metaprograms were recovered that manifest across cancers, this EMT program was enriched in HNSCC cell lines:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.04e-01
Mean rank of genes in gene set: 7825
Rank on gene expression program of genes in gene set:

Adrenergic (Olsen)
Markers listed in on page 3 of the main text of Olsen et al. https://www.biorxiv.org/content/10.1101/2020.05.04.077057v1 - these markers are for adrenergic genes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.13e-01
Mean rank of genes in gene set: 8554.5
Rank on gene expression program of genes in gene set:

Schwann cell precursors (Dong)
Obtained from Fig. 3c of Dong et al. (PMID 32946775).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.14e-01
Mean rank of genes in gene set: 9512
Rank on gene expression program of genes in gene set:

Schwann cell precursors (Kameneva)
Marker gene were obtained from Fig. 1D of Kameneva et al (PMID 33833454). These genes were used by the authors to annotate each cell type in their human fetal adrenal scRNA-seq data obtained 6, 8, 9, 11, 12 and 14 weeks post conception. The authors data suggest these SCPs can give rise to symphathoblasts, which can then give rise to chromaffin cells. However, these SCPs can also directly give rise to chromaffin cells (i.e there are two bridges/paths).:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.14e-01
Mean rank of genes in gene set: 9512
Rank on gene expression program of genes in gene set:

Inflammatory CAF
These marker genes were curated across cancer subtypes in multiple organ systems as reviewed in Lavie et. al. (PMID 35883004) and contain inflammatory specific CAF genes:
Wilcoxon ranksum test P-value for gene set overrepresentation: 8.18e-01
Mean rank of genes in gene set: 6931.12
Rank on gene expression program of genes in gene set: